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Cell No. : Cell Name
RCB2809 : CH12F3-2A update : 2024/08/05 |
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| Comment | Mouse cell line derived from B cell lymphoma. | |||
| Comment from the depositor | ||||
| Terms and conditions | 1) In relation to the use of for-profit institution, a prior written permission of the DEPOSITOR is required. In relation to the use of not-for-profit institution, a prior written permission of the DEPOSITOR is not required. 2) The RECIPENT agrees to use the BIOLOGICAL RESOURCE only for academic research purpose. 3) In publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature ref. (Development 1997;124:4133-4141) designated by the DEPOSITOR is required. 4) Any patenting action by use of the BIOLOGICAL RESORCE requires a prior consent of the DEPOSITOR. 5) Transfer of the BIOLOGICAL REOSRCE to the third party from the RECIPIENT is prohibited without a prior permission of the DEPOSITOR. | |||
| Remarks | ||||
| approver's address |
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| Order Form | Order Form(C-0005.pdf) Approval Form(C-0006.pdf) MTA(C-0007.pdf) MTA(C-0007p.pdf) | |||
| Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp). | ||||
| Basic information | Depositor | Honjo, Tasuku | ||
| Originator | Honjo, Tasuku | |||
| Year of deposit | 2008 | |||
| Animal | _mouse < Mammals | |||
| Tissue | B cell lymphoma | |||
| Classification | cancer | Lifespan | infinite | |
| Morphology | lymphoblast-like | |||
| Cellosaurus(Expasy) | CVCL_E068 | |||
| Medium | Medium List | Culture type | Suspension cells | |
| Culture method | 浮遊細胞の培養に関する一般的な注意(Japanese) | |||
| Culture medium | RPMI1640 + 5% NCTC109 + 20mM HEPES + 10% FBS + 0.05mM 2-Mercaptoethanol | |||
| Antibiotics | Free | Passage method | dilution | Culture information | Passage ratio | 1 : 5 split |
| SC frequency | Subculture : 2 times/week | |||
| Temperature | 37 ℃ | |||
| CO2 concentration | 5 % | |||
| Freeze medium | Medium + 10% DMSO | |||
| Freezing method | Slow freezing | |||
| Mycoplasma/Acholeplasma | (-) | |||
| SSLP(mouse) | OK | |||
| Isozyme | LD, NP | |||
| Images | ||||
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| Reference information | Reference | 1 | ||
| User's Publication | 1 | |||
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