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Cell No. : Cell Name
RCB2809 : CH12F3-2A  update : 2022/11/25
CommentMouse cell line derived from B cell lymphoma.
Comment from the depositor
Terms and conditions1) In relation to the use of for-profit institution, a prior written permission of the DEPOSITOR is required. In relation to the use of not-for-profit institution, a prior written permission of the DEPOSITOR is not required. 2) The RECIPENT agrees to use the BIOLOGICAL RESOURCE only for academic research purpose. 3) In publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature ref. (Development 1997;124:4133-4141) designated by the DEPOSITOR is required. 4) Any patenting action by use of the BIOLOGICAL RESORCE requires a prior consent of the DEPOSITOR. 5) Transfer of the BIOLOGICAL REOSRCE to the third party from the RECIPIENT is prohibited without a prior permission of the DEPOSITOR.
approver's address
Graduate School of Medicine, Kyoto University

Yoshidakonoe-cho, Sakyo-ku, Kyoto 606-8501 Japan
Dr.HONJO Tasuku
Fax. +81-75-753-4388
Order Form Order Form(C-0005.pdf)   Approval Form(C-0006.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor Honjo, Tasuku
Originator Honjo, Tasuku
Year of deposit 2008
Animal mouse < Mammals
Tissue B cell lymphoma
Classification cancer
Lifespan infinite
Morphology lymphoblast-like
Cellosaurus(Expasy) CVCL_E068
deposit info
lot info
Medium Medium List
Culture type Suspension cells
Medium and additives RPMI1640 + 5% NCTC109 + 20mM HEPES + 10% FBS + 0.05mM 2-Mercaptoethanol
Antibiotics Free
Passage method dilution
Culture information Passage ratio 1 : 5 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
SSLP(mouse) OK
Isozyme LD, NP
deposit info
lot info
Reference information Reference 1
User's Publication 1

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2800  Kato H, Taniguchi Y, Kurooka H, Minoguchi S, Sakai T, Nomura-Okazaki S, Tamura K, Honjo T  Involvement of RBP-J in biological functions of mouse Notch1 and its derivatives.  Development  1997  124:4133-41  PubMed ID: 9374409  

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User's Publication
4018  Hanihara-Tatsuzawa F, Miura H, Kobayashi S, Isagawa T, Okuma A, Manabe I, MaruYama T.  Control of Toll-like receptor-mediated T cell-independent type 1 antibody responses by the inducible nuclear protein IκB-ζ.  J Biol Chem  2014  289:30925-30936  PubMed ID: 25124037   DOI: 10.1074/jbc.M114.553230

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