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Cell No. : Cell Name
RCB2808 : Odf2-deficient F9 cells  update : 2023/10/16
CommentA subline of mouse ES cell line, F9 (129), lacking Odf2.
Comment from the depositor
Terms and conditions1) Required written permission from the approver. 2) Use only for science, not use for commercialism. If users are in the same field as ours, the study is to be cooperative study.
Remarks
approver's address
×
English
Address
Osaka University Medical School and Graduate school of Frontier Biosciences
Department Cell Biology
2-2, Yamadaoka, Suita, 565-0871 Japan
Dr.Tsukita, Sachiko
Fax. +81-6-6879-3329
Order Form Order Form(C-0005.pdf)   Approval Form(C-0006.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Tsukita, Sachiko
Originator Tsukita, Sachiko
Year of deposit 2008
Original cell F9
Animal _mouse < Mammals
Strain name 129
Age at sampling embryo
Tissue embryo
Disease name Embryonal carcinoma
Classification mutant
Memo_1 Mycoplasma was eliminated with MC-210.
Lifespan infinite
Morphology other
Cellosaurus(Expasy) CVCL_T374
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Culture medium DMEM (low glucose) + 10% FBS
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 8-20 split
SC frequency Subculture or Medium Renewal : once/2-3 days
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
SSLP(mouse) OK
Isozyme LD, NP
Images
deposit info
lot info
Reference information Reference 2
User's Publication 0


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Reference
5279  Ishikawa H, Kubo A, Tsukita S, Tsukita S.  Odf2-deficient mother centrioles lack distal/subdistal appendages and the ability to generate primary cilia.  Nat Cell Biol  2005  7(5):517-24  PubMed ID: 15852003   DOI: 10.1038/ncb1251
2493  Strickland S, Mahdavi V.  The induction of differentiation in teratocarcinoma stem cells by retinoic acid.  Cell  1978  15(2):393-403  PubMed ID: 214238   DOI: 10.1016/0092-8674(78)90008-9

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