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Cell No. : Cell Name
RCB2806 : MSK-C5.8G  update : 2021/05/20
CommentRat cell line derived from osteosarcoma. F344 strain.
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Kawase, Tomoyuki
Originator Takahashi, Kikuo
Year of deposit 2008
Original cell MSK
Animal rat < Mammals
Strain name F344
Genus Rattus
Species norvegicus
Gender Female
Tissue osteosarcoma
Classification cancer
Lifespan infinite
Morphology other
Cellosaurus(Expasy) CVCL_5336
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives MEM ALPHA + 10% FBS
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Animal PCR OK
Isozyme LD, NP, G6PD, AST
Images
deposit info
lot info
Reference information Reference 1
User's Publication 0


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Reference
5277  Kikuo T. and Kenichi S.  Comparison of osteoblastic markers of clonal cell lines derived from rat osteosarcoma cells.  Journal of Bone and Mineral Metabolism  1991  241-247   

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