Cell No. : Cell Name
RCB2801 : PC12-G11
update : 2024/08/14
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| Comment | A subline of PC-12 expressing synaptotagmin-1, a control cell line for PC12-F7. |
| Comment from the depositor | |
| Terms and conditions | 1) Material is considered the proprietary property of Mitsubishi Kagaku Institute of Life Science (MITILA) and Mitsubishi Chemical Corporation (MCC) . 2) Recipient Investigator agrees not to use Material for profit making or commercial purposes. 3) Recipient Investigator shall acknowledge the source of Material in any publication containing experimental results obtained with the use of Material. 4) At the conclusion of the research for which Material was requested, Recipient shall dispose of unused Material in an appropriate legal manner. 5) After providing Material, RIKEN BRC shall inform MCC of followings;names of Recipient and Recipient Investigator, purpose and date. |
| Remarks | |
| Order Form |
Order Form(C-0005.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
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Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
|
Depositor |
Takayama, Hajime
|
| Originator |
Shoji-Kasai, Yoko
|
| Year of deposit |
2008
|
| Original cell |
PC12h
|
| Animal |
_rat
< Mammals
|
| Genus |
Rattus
|
| Species |
norvegicus
|
| Tissue |
pheochromocytoma
|
| Classification |
cancer
|
| History |
PC12h : Hatanaka, Hiroshi, Osaka University
|
|
Lifespan |
infinite
|
| Morphology |
epithelial-like
|
| Cellosaurus(Expasy) |
CVCL_T753
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deposit info |
lot info |
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Medium |
Medium List |
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Culture type |
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Adherent cells
|
| Culture medium |
|
DMEM (high glucose) + 5% FBS + 5% HS (GIBCO)
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| Antibiotics |
|
Free
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|
Passage method |
|
0.25% Trypsin
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Culture information
|
Passage ratio |
|
1 : 8 split
|
| SC frequency |
|
Subculture : 2 times/week
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| Temperature |
|
37
℃
|
| CO2 concentration |
|
5
%
|
| Freeze medium |
|
Medium + 10% DMSO
|
| Freezing method |
|
Slow freezing
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| Mycoplasma/Acholeplasma |
|
(-)
|
| Animal PCR |
|
OK
|
| Isozyme |
|
LD, NP, AST, PepB
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| Images |
deposit info | lot info |
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| Reference information |
Reference |
2
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| User's Publication |
0
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| Reference |
|
5276
Shoji-Kasai Y, Itakura M, Kataoka M, Yamamori S, Takahashi M.
Protein kinase C-mediated translocation of secretory vesicles to plasma membrane and enhancement of neurotransmitter release from PC12 cells.
Eur J Neurosci
2002
15(8):1390-4
PubMed ID: 11994133
DOI: 10.1046/j.1460-9568.2002.01972.x
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5275
Shoji-Kasai Y, Yoshida A, Sato K, Hoshino T, Ogura A, Kondo S, Fujimoto Y, Kuwahara R, Kato R, Takahashi M.
Neurotransmitter release from synaptotagmin-deficient clonal variants of PC12 cells.
Science
1992
256(5065):1821-3
PubMed ID: 1352065
DOI: 10.1126/science.256.5065.1820
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