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Cell No. : Cell Name
RCB2800 : PC12-F7  update : 2023/10/16
CommentA subline of PC-12 lacking synaptotagmin-1.
Comment from the depositor
Terms and conditions1) Material is considered the proprietary property of Mitsubishi Kagaku Institute of Life Science (MITILA) and Mitsubishi Chemical Corporation (MCC) . 2) Recipient Investigator agrees not to use Material for profit making or commercial purposes. 3) Recipient Investigator shall acknowledge the source of Material in any publication containing experimental results obtained with the use of Material. 4) At the conclusion of the research for which Material was requested, Recipient shall dispose of unused Material in an appropriate legal manner. 5) After providing Material, RIKEN BRC shall inform MCC of followings;names of Recipient and Recipient Investigator, purpose and date.
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor Takayama, Hajime
Originator Shoji-Kasai, Yoko
Year of deposit 2008
Original cell PC12h
Animal _rat < Mammals
Genus Rattus
Species norvegicus
Tissue pheochromocytoma
Classification cancer
History PC12h : Hatanaka, Hiroshi, Osaka University
Lifespan infinite
Morphology epithelial-like
Cellosaurus(Expasy) CVCL_T752
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Culture medium DMEM (high glucose) + 5% FBS + 5% HS (GIBCO)
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Animal PCR OK
Isozyme LD, NP, AST, PepB
deposit info
lot info
Reference information Reference 1
User's Publication 1

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5275  Shoji-Kasai Y, Yoshida A, Sato K, Hoshino T, Ogura A, Kondo S, Fujimoto Y, Kuwahara R, Kato R, Takahashi M.  Neurotransmitter release from synaptotagmin-deficient clonal variants of PC12 cells.  Science  1992  256(5065):1821-3  PubMed ID: 1352065   DOI: 10.1126/science.256.5065.1820

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User's Publication
19497  Bello OD, Jouannot O, Chaudhuri A, Stroeva E, Coleman J, Volynski KE, Rothman JE, Krishnakumar SS.  Synaptotagmin oligomerization is essential for calcium control of regulated exocytosis  Proc Natl Acad Sci U S A   2018  115(32):E7624-E7631.  PubMed ID: 30038018   DOI: 10.1073/pnas.1808792115

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