Cell No. : Cell Name RCB2783 : RGC-6  update : 2023/10/16
Comment			Rat cell line derived from glioma induced by N-nitrosomethylurea. TKG0242 (Deposited from Tohoku Univ.).
Comment from the depositor
Terms and conditions			There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form			Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)
			Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
	Basic information	Depositor	Fukuda, Hiroshi
		Originator	Benda
		Year of deposit	2008
		Original cell	TKG0242
		Animal	_rat < Mammals
		Tissue	brain
		Classification	cancer
		History	Sato, Y. → Cell Resource Center for Biomedical Research, Tohoku University（TKG0242）
		Lifespan	infinite
		Morphology	epithelial-like
		Cellosaurus(Expasy)	CVCL_0194
			deposit info	lot info
		Medium	Medium List
		Culture type		Adherent cells
		Culture medium		MEM + 10% FBS
		Antibiotics		Free
		Passage method		0.25% Trypsin
	Culture information	Passage ratio		1 : 8 split
		SC frequency		Subculture : 2 times/week
		Temperature		37 ℃
		CO2 concentration		5 %
		Freeze medium		Medium + 10% DMSO
		Freezing method		Slow freezing
		Mycoplasma		(-)
		Animal PCR		OK
		Isozyme		LD, NP, G6PD, AST
Images			deposit info	lot info
Reference information		Reference	3
		User's Publication	2

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Reference

5272  Bondareff W, Tuttle RL.  Cortical cytoplasm and configurational changes of RGC-6 cells exposed to cytochalasin B.  Am J Anat  1975  144(3):257-73  PubMed ID: 1211367   DOI: 10.1002/aja.1001440302

5271  Dawson G, Kemp SF, Stoolmiller AC, Dorfman A, Kennedy JP Jr.  Biosynthesis of glycosphingolipids by mouse neuroblastoma (NB41A), rat glia (RGC-6) and human glia (CHB-4) in cell culture.  Biochem Biophys Res Commun  1971  44(3):687-94  PubMed ID: 5166263   DOI: 10.1016/s0006-291x(71)80138-9

645  Benda P, Lightbody J, Sato G, Levine L, Sweet W.  Differentiated rat glial cell strain in tissue culture.  Science  1968  161(3839):370-1  PubMed ID: 4873531   DOI: 10.1126/science.161.3839.370

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User's Publication

6928  Aoki M, Watabe T, Nagamori S, Naka S, Ikeda H, Kongpracha P, Horitsugi G, Kanai Y, Shimosegawa E, Kanai Y, Hatazawa J.  Distribution of LAT1-targeting PET tracer was independent of the tumor blood flow in rat xenograft models of C6 glioma and MIA PaCa-2.  Ann Nucl Med  2019  33(6):394-403  PubMed ID: 30820863   DOI: 10.1007/s12149-019-01346-9

17088  Hanaoka K, Watabe T, Naka S, Kanai Y, Ikeda H, Horitsugi G, Kato H, Isohashi K, Shimosegawa E, Hatazawa J.  FBPA PET in boron neutron capture therapy for cancer: prediction of (10)B concentration in the tumor and normal tissue in a rat xenograft model  EJNMMI Res  2014  4(1):70  PubMed ID: 25621196   DOI: 10.1186/s13550-014-0070-2