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Cell No. : Cell Name
RCB2782 : RBL 2H3  update : 2022/12/21
CommentRat cell line derived from basophilic leukemia cells. TKG0321 (Deposited from Tohoku Univ.).
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor Fukuda, Hiroshi
Originator Siraganian, R. & Eccleston.
Year of deposit 2008
Original cell TKG0321
Animal rat < Mammals
Tissue peripheral blood
Classification cancer
History Maeyama, K. & Tanno, Y. → Cell Resource Center for Biomedical Research, Tohoku University(TKG0321)
Lifespan infinite
Morphology other
Cellosaurus(Expasy) CVCL_0591
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives MEM + 10% FBS
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Animal PCR OK
Isozyme LD, NP, G6PD, AST
deposit info
lot info
Reference information Reference 3
User's Publication 27

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5269  Morita Y, Siraganian RP.  Inhibition of IgE-mediated histamine release from rat basophilic leukemia cells and rat mast cells by inhibitors of transmethylation.  J Immunol  1981  127(4):1339-44  PubMed ID: 6168684  
5268  Anthony Kulczycki, Chaviva Isersky, Henry Metzger  THE INTERACTION OF IgE WITH RAT BASOPHILIC LEUKEMIA CELLS. I. EVIDENCE FOR SPECIFIC BINDING 0]~ IgE   J Exp Med  1974  139(3):600-16  PubMed ID: 4812630   DOI: 10.1084/jem.139.3.600
5267  Eccleston E, Leonard BJ, Lowe JS, Welford HJ.  Basophilic leukaemia in the albino rat and a demonstration of the basopoietin.  Nat New Biol  1973  244(133):73-6  PubMed ID: 4516144   DOI: 10.1038/newbio244073b0

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User's Publication
13447  Lin YANG, Xianwei ZHU, Minoru SUGA, Hiroaki SHINOHARA  Evaluation of the Anti-Allergic Effect of Natural Medicines on Mast Cell by Using Two-Dimensional Surface Plasmon Resonance Observation  Electrochemistry  2021      DOI: 10.5796/electrochemistry.20-00113
16772  Mohammad Abdul ALIM, Minoru SUGA, Hiroaki SHINOHARA  Rapid and Highly Sensitive Electrochemical Technique for Cell Viability Assay via Monitoring of Intracellular NADH with New Double Mediator System  Electrochemistry  2021  89:579-584    DOI: 10.5796/electrochemistry.21-00088
11451  Kawamoto Y, Kondo H, Hasegawa M, Kurimoto C, Ishii Y, Kato C, Botei T, Shinya M, Murate T, Ueno Y, Kawabe M, Goto Y, Yamamoto R, Iida M, Yajima I, Ohgami N, Kato M, Takeda K.  Inhibition of mast cell degranulation by melanin.  Biochem. Pharmacol.  2019    PubMed ID: 30796915   DOI: 10.1016/j.bcp.2019.02.015
11670  Xiaolu He, Akiko Yamauchi, Toshiki Nakano, Toshiyasu Yamaguchi, Yoshihiro Ochiai  The composition and anti-inflammatory effect of polysaccharides from the red alga Chondrus verrucosus  Fisheries Science  2019      DOI: 10.1007/s12562-019-01336-w
10538  Mwakalu kwa, Ahmed Ashour, Yhiya Amen, Yasuharu Niwa, Sonam Tamrakar, Tomofumi Miyamoto, Kuniyoshi Shimizu  Anti-allergic activity of polyphenolic compounds isolated from olive mill wastes  Journal of Functional Foods  2019  58:207-217    DOI: 10.1016/j.jff.2019.04.058
9997  Kamei R, Fujimura T, Matsuda M, Kakihara K, Hirakawa N, Baba K, Ono K, Arakawa K, Kawamoto S.  A flavanone derivative from the Asian medicinal herb (Perilla frutescens) potently suppresses IgE-mediated immediate hypersensitivity reactions.  Biochem. Biophys. Res. Commun.  2017  483:674-679  PubMed ID: 27986566   DOI: 10.1016/j.bbrc.2016.12.083
10063  Kishikawa A, Amen Y, Shimizu K.  Anti-allergic triterpenes isolated from olive milled waste.  Cytotechnology  2017  69:307-315  PubMed ID: 28074390   DOI: 10.1007/s10616-016-0058-z
10307  Tan H, Sonam T, Shimizu K.  The Potential of Triterpenoids from Loquat Leaves (Eriobotrya japonica) for Prevention and Treatment of Skin Disorder.  Int J Mol Sci  2017  18:  PubMed ID: 28492484   DOI: 10.3390/ijms18051030
7140  Shi YH, Zhu S, Ge YW, He YM, Kazuma K, Wang Z, Yoshimatsu K, Komatsu K.  Monoterpene derivatives with anti-allergic activity from red peony root, the root of Paeonia lactiflora.  Fitoterapia  2016  108:55-61  PubMed ID: 26598138   DOI: 10.1016/j.fitote.2015.11.011
7145  Nakakita S, Itoh A, Nakakita Y, Nonaka Y, Ogawa T, Nakamura T, Nishi N.  Cooperative Interactions of Oligosaccharide and Peptide Moieties of a Glycopeptide Derived from IgE with Galectin-9.  J. Biol. Chem.  2016  291:968-79  PubMed ID: 26582205   DOI: 10.1074/jbc.M115.694448
9552  Shi YH, Zhu S, Tamura T, Kadowaki M, Wang Z, Yoshimatsu K, Komatsu K.  Chemical constituents with anti-allergic activity from the root of Edulis Superba, a horticultural cultivar of Paeonia lactiflora.  J Nat Med  2016  70:234-40  PubMed ID: 26833191   DOI: 10.1007/s11418-016-0966-4
10857    Clinical Evaluation of Novel Natural Polysaccharides Sacran as a Skincare Material for Atopic Dermatitis Patients   Journal of Cosmetics, Dermatological Sciences and Applications  2016  6, 9-18    DOI: 10.4236/jcdsa.2016.61002
7437  Kishikawa A, Ashour A, Zhu Q, Yasuda M, Ishikawa H, Shimizu K.  Multiple Biological Effects of Olive Oil By-products such as Leaves, Stems, Flowers, Olive Milled Waste, Fruit Pulp, and Seeds of the Olive Plant on Skin.  Phytother Res  2015  29:877-86  PubMed ID: 25779104   DOI: 10.1002/ptr.5326
7473  Sato A, Tamura H.  High antiallergic activity of 5,6,4'-trihydroxy-7,8,3'-trimethoxyflavone and 5,6-dihydroxy-7,8,3',4'-tetramethoxyflavone from eau de cologne mint (Mentha×piperita citrata).  Fitoterapia  2015  102:74-83  PubMed ID: 25704366   DOI: 10.1016/j.fitote.2015.02.003
7520  Akihiko Sato, Ting Zhang, Lina Yonekura, Hirotoshi Tamura.  Antiallergic activities of eleven onions (Allium cepa) were attributed to quercetin 4'-glucoside using QuEChERS method and Pearson's correlation coefficient.  J Functional Foods  2015  14:581-589    DOI: 10.1016/j.jff.2015.02.029
7540  Kobayashi S, Kato T, Azuma T, Kikuzaki H, Abe K..  Anti-allergenic activity of polymethoxyflavones from Kaempferia parviflora.  J Functional Foods  2015  13:100-107    DOI: 10.1016/j.jff.2014.12.029
7554  Q. Zhu, T. Nakagawa, A. Kishikawa, K. Ohnuki, K. Shimizu  In vitro bioactivities and phytochemical profile of various parts of the strawberry (Fragaria × ananassa var.?Amaou).  J Functional Foods  2015  13:38-49    DOI: 10.1016/j.jff.2014.12.026
10950  Akihiko, Sato et al  Antialleric Activity of varius kinds of vegetables and Fruits in japan and thailand  Conference: Journal of Science and Technology  2014  52B, 726-735   
10261  Mitsuteru Irie, Junkyu Han, Atsushi Kawachi, Jamila Tarhouni, Mohamed Ksibi, Kenichi Kashiwagi & Hiroko Isoda  In Vitro Testing and Commercialization Potential of Extracted Fulvic Acid from Dredged Sediment from Arid Region Reservoirs  Waste and Biomass Valorization  2014  5:273–281    DOI: 10.1007/s12649-013-9234-y
9479  Amira Mira, Akinobu Tanaka, Yumie Tateyama, Ryuichiro Kondo, Kuniyoshi Shimizu  Comparative Biological Study of Roots, Stems, Leaves, and Seeds of Angelica Shikokiana Makino  J Ethnopharmacol  2013  148(3):980-7  PubMed ID: 23769982   DOI: 10.1016/j.jep.2013.06.008
15743  Yamaura K, Ishiwatari M, Yamamoto M, Shimada M, Bi Y, Ueno K.  Anthocyanins, but not anthocyanidins, from bilberry (Vaccinium myrtillus L.) alleviate pruritus via inhibition of mast cell degranulation  J Food Sci  2012  77(12):H262-7  PubMed ID: 23164040   DOI: 10.1111/j.1750-3841.2012.02974.x
15996  Morimoto-Kamata R, Mizoguchi S, Ichisugi T, Yui S.  Cathepsin G induces cell aggregation of human breast cancer MCF-7 cells via a 2-step mechanism: catalytic site-independent binding to the cell surface and enzymatic activity-dependent induction of the cell aggregation  Mediators Inflamm  2012  2012:456462  PubMed ID: 22919124   DOI: 10.1155/2012/456462
7986  Kawamoto S, Kaneoke M, Ohkouchi K, Amano Y, Takaoka Y, Kume K, Aki T, Yamashita S, Watanabe K, Kadowaki M, Hirata D, Ono K.  Sake lees fermented with lactic acid bacteria prevents allergic rhinitis-like symptoms and IgE-mediated basophil degranulation.  Biosci. Biotechnol. Biochem.  2011  75:140-4  PubMed ID: 21228477   DOI: 10.1271/bbb.100541
8680  Parida Yamada, Moktar Zarrouk, Hiroko Isoda  Screening of Various Tunisian Olive Oils for Their Inhibitory Effect on Beta-Hexosaminidase Release by Basophilic Cells  Animal Cell Technology: Basic & Applied Aspects  2009  231-238:    DOI: 10.1007/978-1-4020-9646-4_36
5880  Rieko IIJIMA1, Parida YAMADA1, Junkyu HAN1, Hideyuki SHIGEMORI, Hiroko ISODA  Anti-allergic Effect of Acteoside Derived from Arid Land Plant Cistanche tubulosa  Journal of Arid Land Studies   2009  9-1:383- 386   
12784  Yamada P, Zarrouk M, Kawasaki K, Isoda H.  Inhibitory effect of various Tunisian olive oils on chemical mediator release and cytokine production by basophilic cells  J Ethnopharmacol  2008  116(2):279-87  PubMed ID: 18178046   DOI: 10.1016/j.jep.2007.11.028
14933  Murakami M, Yoshihara K, Shimbara S, Lambeau G, Singer A, Gelb MH, Sawada M, Inagaki N, Nagai H, Kudo I.  Arachidonate release and eicosanoid generation by group IIE phospholipase A(2)  Biochem Biophys Res Commun  2002  292(3):689-96  PubMed ID: 11922621   DOI: 10.1006/bbrc.2002.6716

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