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Cell No. : Cell Name
RCB2776 : P388  update : 2023/11/07
CommentMouse cell line derived from lymphoid tumor. DBA/2 strain. TKG0326 (Deposited from Tohoku Univ.).
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor Fukuda, Hiroshi
Year of deposit 2008
Original cell TKG0326
Animal _mouse < Mammals
Strain name DBA/2
Tissue lymphoid tumor
Classification cancer
History Cell Resource Center for Biomedical Research, Tohoku University(TKG0326)
Lifespan infinite
Morphology lymphoblast-like
Cellosaurus(Expasy) CVCL_7222
deposit info
lot info
Medium Medium List
Culture type Suspension cells
Culture medium RPMI1640 + 10% FBS
Antibiotics Free
Passage method pipetting or 0.25% Trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
SSLP(mouse) OK
Isozyme LD, NP
deposit info
lot info
Reference information Reference 5
User's Publication 2

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5265  Mizel SB, Oppenheim JJ, Rosenstreich DL.  Characterization of lymphocyte-activating factor (LAF) produced by the macrophage cell line, P388D1. I. Enhancement of LAF production by activated T lymphocytes.  J Immunol  1978  120(5):1497-503  PubMed ID: 149171  
716  Ralph P, Nakoinz I  Antibody-dependent killing of erythrocyte and tumor targets by macrophage-related cell lines: enhancement by PPD and LPS.  J Immunol  1977  119:950-54  PubMed ID: 894031  
1141  Ralph P, Moore, M A, Nilsson K  Lysozyme synthesis by established human and murine histiocytic lymphoma cell lines.  J Exp Med  1976  143:1528-33  PubMed ID: 1083890   DOI: 10.1084/jem.143.6.1528
5264  EAGLE H.  METABOLIC CONTROLS IN CULTURED MAMMALIAN CELLS.  Science  1965  148(3666):42-51  PubMed ID: 14258726   DOI: 10.1126/science.148.3666.42
5266  ZWILLENBERG LO.  The dendritic cell system and mast cells in non-epidermal stratified squamous epithelium.  Nature  1958  181(4619):1343-4  PubMed ID: 13541472   DOI: 10.1038/1811343b0

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User's Publication
10568  Tsuruoka T, Ishikawa K, Hosoe T, Davaajab D, Duvjir S, Surenjav U.  A new cinnamoylphenethylamine derivative from a Mongolian Allium species, Allium carolinianum.  J Nat Med  2018  72:332-334  PubMed ID: 29063363   DOI: 10.1007/s11418-017-1142-1
4435  Sou Kikuchi et al  Isolation and structure determination of lysiformine from bacteria associated with marine sponge Halichondria okadai  Tetrahedron  2018  74, 3742-3747    DOI: 10.1016/j.tet.2018.05.049

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