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Cell No. : Cell Name
RCB2767 : NIH/3T3  update : 2024/02/16
CommentMouse fibroblast-like cell line. TKG0299 (Deposited from Tohoku Univ.). Application consideration
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor Fukuda, Hiroshi
Originator Todaro, G. J.
Year of deposit 2008
Original cell TKG0299
Animal _mouse < Mammals
Strain name NIH Swiss
Age at sampling embryo
Tissue embryo
Classification other
History Cell Resource Center for Biomedical Research, Tohoku University(TKG0299)
Lifespan infinite
Morphology fibroblast-like
Cellosaurus(Expasy) CVCL_0594
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Culture medium DMEM (low glucose) + 10% CS
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage cell No 3 x 10 5 cells/60 mm dish
SC frequency Subculture : once/3-4 days
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Animal PCR OK
SSLP(mouse) OK
deposit info
lot info
Reference information Reference 2
User's Publication 54

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5004  Benveniste RE, Lieber MM, Todaro GJ.  A distinct class of inducible murine type-C viruses that replicates in the rabbit SIRC cell line.  Proc Natl Acad Sci U S A.  1974  71(3):602-6  PubMed ID: 4132531   DOI: 10.1073/pnas.71.3.602
298  Jainchill, J L, Aaronson, S A, Todaro, G J  Murine sarcoma and leukemia viruses: assay using clonal lines of contact-inhibited mouse cells.  J Virol  1969  4:549-53  PubMed ID: 4311790   DOI: 10.1128/JVI.4.5.549-553.1969

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User's Publication
21649  Tsuchida A, Kaneko T, Nishikawa K, Kawasaki M, Yokokawa R, Shintaku H.  Opto-combinatorial indexing enables high-content transcriptomics by linking cell images and transcriptomes.  Lab Chip  2024    PubMed ID: 38506394   DOI: 10.1039/d3lc00866e
19550  Oka N, Shimada K, Ishii A, Kobayashi N, Kondo K.  SARS-CoV-2 S1 protein causes brain inflammation by reducing intracerebral acetylcholine production  iScience  2023  26(6):106954  PubMed ID: 37275532   DOI: 10.1016/j.isci.2023.106954
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20844  Suzuki M, Kimura T, Nakano Y, Kobayashi M, Okada M, Matsumoto T, Nakamura N, Hashimoto Y, Kishida A.  Preparation of mineralized pericardium by alternative soaking for soft-hard interregional tissue application.  J Biomed Mater Res A  2023  111(2):198-208  PubMed ID: 36069375   DOI: 10.1002/jbm.a.37445
21209  Zhang Z, Li Q, Hatakeyama M, Kitaoka T.  Injectable cell-laden hydrogels fabricated with cellulose and chitosan nanofibers for bioprinted liver tissues.  Biomed Mater  2023    PubMed ID: 37168003   DOI: 10.1088/1748-605X/acd49a
21443  Liu Q, Li Q, Hatakeyama M, Kitaoka T.  Proliferation and differential regulation of osteoblasts cultured on surface-phosphorylated cellulose nanofiber scaffolds.  Int J Biol Macromol  2023  253(Pt 3):126842  PubMed ID: 37703974   DOI: 10.1016/j.ijbiomac.2023.126842
17584  Suzuki M, Kimura T, Yoshida Y, Kobayashi M, Hashimoto Y, Takahashi H, Shimizu T, Anzai S, Nakamura N, Kishida A.  In Vitro Tissue Reconstruction Using Decellularized Pericardium Cultured with Cells for Ligament Regeneration  Polymers (Basel)  2022  14(12):2351  PubMed ID: 35745927   DOI: 10.3390/polym14122351
19504  Yasuda K, Watanabe TM, Kang MG, Seo JK, Rhee HW, Tate SI.  Valosin-containing protein regulates the stability of fused in sarcoma granules in cells by changing ATP concentrations  FEBS Lett  2022  596(11):1412-1423  PubMed ID: 35445401   DOI: 10.1002/1873-3468.14353
20606  Noda T, Hatakeyama M, Kitaoka T.  Combination of Polysaccharide Nanofibers Derived from Cellulose and Chitin Promotes the Adhesion, Migration and Proliferation of Mouse Fibroblast Cells.  Nanomaterials (Basel)  2022  12(3)  PubMed ID: 35159746   DOI: 10.3390/nano12030402
16435  Qi Li, Mayumi Hatakeyama, Takuya Kitaoka  Bioadaptive Porous 3D Scaffolds Comprising Cellulose and Chitosan Nanofibers Constructed by Pickering Emulsion Templating  Advanced Functional Materials  2022  2200249    DOI: 10.1002/adfm.202200249
20758  Horikoshi S, Iwabuchi M, Kawaguchi M, Yasumasu S, Serpone N.  Uptake of nanoparticles from sunscreen physical filters into cells arising from increased environmental microwave radiation: increased potential risk of the use of sunscreens to human health.  Photochem Photobiol Sci  2022    PubMed ID: 35781788   DOI: 10.1007/s43630-022-00259-3
16919  Aytemiz DG, Kambe Y, Hirata M, Nishi H, Kameda T.  Effects of RGD-fused silk fibroin in a solution format on fibroblast proliferation and collagen production  Biomed Mater Eng  2022  Jul 15  PubMed ID: 35871317   DOI: 10.3233/BME-221430
16921  unoki S, Hatayama H, Ohyabu Y, Kobayashi K.  Fibril matrices created with collagen from the marine fish barramundi for use in conventional three-dimensional cell culture  Int J Biol Macromol  2022  203:361-36  PubMed ID: 35074336   DOI: 10.1016/j.ijbiomac.2022.01.098
20879  Tamura A, Kang TW, Tonegawa A, Arisaka Y, Masuda H, Mikami R, Iwata T, Yoda T, Yui N.  Supramolecular Surface Coatings with Acetylated Polyrotaxane-Based Triblock Copolymers for Thermal Regulation of Cell Adhesion and Fabrication of Cell Sheets.  Biomacromolecules  2022    PubMed ID: 36206115   DOI: 10.1021/acs.biomac.2c01043
20883  Wang S, Tanaka Y, Xu Y, Takeda S, Hirokawa N.  KIF3B promotes a PI3K signaling gradient causing changes in a Shh protein gradient and suppressing polydactyly in mice.  Dev Cell  2022  57(19):2273-2289.e11  PubMed ID: 36220081   DOI: 10.1016/j.devcel.2022.09.007
20939  Toki E, Goto S, Setoguchi S, Terada K, Watase D, Yamakawa H, Yamada A, Koga M, Kubota K, Iwasaki K, Karube Y, Matsunaga K, Takata J.  Delivery of the reduced form of vitamin K2(20) to NIH/3T3 cells partially protects against rotenone induced cell death.  Sci Rep  2022  12(1):19878  PubMed ID: 36400879   DOI: 10.1038/s41598-022-24456-3
20070  Mukaida A, Adachi R, Akiyama Y, Kamimura M.  Facile Preparation of a Hairpin DNA-Gold Nanoparticle Monoconjugate with a Single-Dye Molecule and Lactobionic Acid as Targeting Ligand.  Anal Sci  2021    PubMed ID: 33678725   DOI: 10.2116/analsci.20SCN07
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15494  Mayumi Hatakeyama, Takuya Kitaoka  Surface-Carboxylated Nanocellulose-Based Bioadaptive Scaffolds for Cell Culture  Cellulose  2021  25 August    DOI: 10.1007/s10570-021-04154-5
20453  Sasaki D, Kusamori K, Takayama Y, Itakura S, Todo H, Nishikawa M.  Development of nanoparticles derived from corn as mass producible bionanoparticles with anticancer activity.  Sci Rep  2021  11(1):22818  PubMed ID: 34819568   DOI: 10.1038/s41598-021-02241-y
20479  Kawabata K, Totani M, Kawaguchi D, Matsuno H, Tanaka K.  Two-Dimensional Cellular Patterning on a Polymer Film Based on Interfacial Stiffness.  Langmuir  2021    PubMed ID: 34902971   DOI: 10.1021/acs.langmuir.1c02776
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16927  Honda F, Tsuboi H, Ono Y, Abe S, Takahashi H, Ito K, Yamada K, Kawano M, Kondo Y, Asano K, Tanaka M, Malissen M, Malissen B, Matsumoto I, Sumida T.  Pathogenic roles and therapeutic potential of the CCL8-CCR8 axis in a murine model of IgG4-related sialadenitis  Arthritis Res Ther  2021  23(1):214.  PubMed ID: 34391459   DOI: 10.1186/s13075-021-02597-6
13222  Watanabe H, Akiyama Y.  Improved and reproducible cell viability in the superflash freezing method using an automatic thawing apparatus.  Cryobiology  2020    PubMed ID: 32946776   DOI: 10.1016/j.cryobiol.2020.09.003
14842  Okawa M, Sakoda M, Ohta S, Hasegawa K, Yatomi Y, Ito T.  The Balance between the Hemostatic Effect and Immune Response of Hyaluronan Conjugated with Different Chain Lengths of Inorganic Polyphosphate  Biomacromolecules  2020  21(7):2695-2704  PubMed ID: 32453578   DOI: 10.1021/acs.biomac.0c00390
11126  Goda T, Miyahara Y.  Electrodeposition of Zwitterionic PEDOT Films for Conducting and Antifouling Surfaces.  Langmuir  2019  35(5):1126-1133  PubMed ID: 30001621   DOI: 10.1021/acs.langmuir.8b01492
11362  Hatano H, Goda T, Matsumoto A, Miyahara Y.  Induced Proton Perturbation for Sensitive and Selective Detection of Tight Junction Breakdown.  Anal. Chem.  2019    PubMed ID: 30596415   DOI: 10.1021/acs.analchem.8b05237
11398  Razi MA, Wakabayashi R, Goto M, Kamiya N.  Self-Assembled Reduced Albumin and Glycol Chitosan Nanoparticles for Paclitaxel Delivery.  Langmuir  2019    PubMed ID: 30673276   DOI: 10.1021/acs.langmuir.8b02809
11440  Ono R, Yasuhiko Y, Aisaki KI, Kitajima S, Kanno J, Hirabayashi Y.  Exosome-mediated horizontal gene transfer occurs in double-strand break repair during genome editing.  Commun Biol  2019    PubMed ID: 30775458   DOI: 10.1038/s42003-019-0300-2
11479  Hasan M, Hama S, Kogure K.  Low Electric Treatment activates Rho GTPase via Heat Shock Protein 90 and Protein Kinase C for Intracellular Delivery of siRNA.  Sci Rep  2019    PubMed ID: 30858501   DOI: 10.1038/s41598-019-40904-z
11543  Oki Y, Kirita K, Ohta S, Ohba S, Horiguchi I, Sakai Y, Ito T.  Switching of Cell Proliferation/Differentiation in Thiol-Maleimide Clickable Microcapsules Triggered by in Situ Conjugation of Biomimetic Peptides.  Biomacromolecules  2019    PubMed ID: 31059241   DOI: 10.1021/acs.biomac.9b00333
11580  Tanaka J, Mabuchi Y, Hata K, Yasuhara R, Takamatsu K, Kujiraoka S, Yukimori A, Takakura I, Sumimoto H, Fukada T, Azuma M, Akiyama H, Nishimura R, Shimane T, Mishima K.  Sox9 regulates the luminal stem/progenitor cell properties of salivary glands.  Exp. Cell Res.  2019    PubMed ID: 31153924   DOI: 10.1016/j.yexcr.2019.05.030
11684  Pan Qi, Ying Grace Zheng, Seiichi Ohta, Norihiro Kokudo, Kiyoshi Hasegawa, Taichi Ito  In Situ Fabrication of Double-Layered Hydrogels via Spray Processes to Prevent Postoperative Peritoneal Adhesion  ACS Biomaterials Science & Engineering  2019    PubMed ID: 33448821   DOI: 10.1021/acsbiomaterials.9b00791
11773  Kano A, Iwasaki T, Shindo M.  Bongkrekic acid facilitates glycolysis in cultured cells and induces cell death under low glucose conditions.  Biochem Biophys Rep  2019    PubMed ID: 31517068   DOI: 10.1016/j.bbrep.2019.100683
12328  Ikeda T, Ichikawa K, Shigeto H, Ishida T, Hirota R, Funabashi H, Kuroda A.  Arginine-mediated dissociation of single cells and cell sheets from a polystyrene culture dish  Biosci Biotechnol Biochem  2019  83(12):2272-2275  PubMed ID: 31482750   DOI: 10.1080/09168451.2019.1659716
12530  Takatori Y, Uraoka T, Narita T, Yunoki S, Yahagi N.  Potential of temperature-response collagen-genipin sols as a novel submucosal injection material for endoscopic resection  Endosc Int Open  2019  7(4):E561-E567  PubMed ID: 30957006   DOI: 10.1055/a-0867-9450
12531  Sumida R, Maeda T, Kawahara I, Yusa J, Kato Y.  Platelet-rich fibrin increases the osteoprotegerin/receptor activator of nuclear factor-κB ligand ratio in osteoblasts  Exp Ther Med  2019  18(1):358-365  PubMed ID: 31258673   DOI: 10.3892/etm.2019.7560
10786  He Q, Okajima T, Onoe H, Subagyo A, Sueoka K, Kuribayashi-Shigetomi K.  Origami-based self-folding of co-cultured NIH/3T3 and HepG2 cells into 3D microstructures.  Sci Rep  2018  8:4556  PubMed ID: 29540810   DOI: 10.1038/s41598-018-22598-x
11165  Yang YJ, Choi YS, Cha HJ.  Bioinspired Load-Bearing Hydrogel Based on Engineered Sea Anemone Skin-Derived Collagen-Like Protein.  Biotechnol J  2018  13(12):e1800086  PubMed ID: 30102020   DOI: 10.1002/biot.201800086
4432  Yuya Morimoto et al  Three-dimensional printed microfluidic modules for design changeable coaxial microfluidic devices  Sensors and Actuators B: Chemical  2018  274, 491-500    DOI: 10.1016/j.snb.2018.07.151
14914  Okazaki T, Tamai K, Shibuya R, Nakamura M, Mochizuki M, Yamaguchi K, Abe J, Takahashi S, Sato I, Kudo A, Okada Y, Satoh K.  Periostin is a negative prognostic factor and promotes cancer cell proliferation in non-small cell lung cancer  Oncotarget  2018  9(58):31187-31199  PubMed ID: 30131847   DOI: 10.18632/oncotarget.25435
9971  Kitai Y, Zhang X, Hayashida Y, Kakehi Y, Tamura H.  Induction of G2/M arrest and apoptosis through mitochondria pathway by a dimer sesquiterpene lactone from Smallanthus sonchifolius in HeLa cells.  J Food Drug Anal  2017  25:619-627  PubMed ID: 28911648   DOI: 10.1016/j.jfda.2016.10.005
14671  Wathoni N, Motoyama K, Higashi T, Okajima M, Kaneko T, Arima H.  Enhancement of curcumin wound healing ability by complexation with 2-hydroxypropyl-γ-cyclodextrin in sacran hydrogel film  Int J Biol Macromol  2017  98:268-276  PubMed ID: 28161536   DOI: 10.1016/j.ijbiomac.2017.01.144
15680  Yoshiyuki Nakagawa, Seiichi Ohta, Akira Sugahara, Masashi Okubo, Atsuo Yamada, Taichi Ito  In Vivo Redox-Responsive Sol–Gel/Gel–Sol Transition of Star Block Copolymer Solution Based on Ionic Cross-Linking  Macromolecules  2017  50:5539-5548    DOI: 10.1021/acs.macromol.7b01020
10009  Makino S, Fukumura R, Gondo Y.  Illegitimate translation causes unexpected gene expression from on-target out-of-frame alleles created by CRISPR-Cas9.  Sci Rep  2016  6:39608  PubMed ID: 28000783   DOI: 10.1038/srep39608
12813  Wathoni N, Motoyama K, Higashi T, Okajima M, Kaneko T, Arima H.  Physically crosslinked-sacran hydrogel films for wound dressing application  Int J Biol Macromol  2016  89:465-70  PubMed ID: 27151668   DOI: 10.1016/j.ijbiomac.2016.05.006
13473  Komatsu Y, Ibi M, Chosa N, Kyakumoto S, Kamo M, Shibata T, Sugiyama Y, Ishisaki A.  Zoledronic acid suppresses transforming growth factor-β-induced fibrogenesis by human gingival fibroblasts  Int J Mol Med  2016  38(1):139-47  PubMed ID: 27176567   DOI: 10.3892/ijmm.2016.2582
14211  Shimada I, Kubota A, Katoh M, Suzuki F.  Hyperoxia causes diffuse alveolar damage through mechanisms involving upregulation of c-Myc/Bax and enhanced production of reactive oxygen species  Respir Investig  2016  54(1):59-68  PubMed ID: 26718146   DOI: 10.1016/j.resinv.2015.08.006
12257  Shinya Mitsuhashi, Chihaya Shindo, Kengo Shigetomi, Toshizumi Miyamoto, Makoto Ubukata  (+)-Epogymnolactam, a novel autophagy inducer from mycelial culture of Gymnopus sp  Phytochemistry  2015  114:163-7  PubMed ID: 25242622   DOI: 10.1016/j.phytochem.2014.08.012
15431  Ryosuke Takahashi, Takaharu Okajima  Mapping power-law rheology of living cells using multi-frequency force modulation atomic force microscopy  Applied Physics Letters  2015  107:173702    DOI: 10.1063/1.4934874
14947  Yunoki S, Hatayama H, Ebisawa M, Kondo E, Yasuda K.  A novel fabrication method to create a thick collagen bundle composed of uniaxially aligned fibrils: an essential technology for the development of artificial tendon/ligament matrices  J Biomed Mater Res A  2015  103(9):3054-65  PubMed ID: 25727567   DOI: 10.1002/jbm.a.35440
3589  Nakagawa Y, Nakasako S, Ohta S, Ito T.  A biocompatible calcium salt of hyaluronic acid grafted with polyacrylic acid  Carbohydr Polym  2015  117:43-53  PubMed ID: 25498607   DOI: 10.1016/j.carbpol.2014.09.037
11541  Alves F, Oliveira FS, Schröder B, Matos C, Marrucho IM.  Synthesis, characterization, and liposome partition of a novel tetracycline derivative using the ionic liquids framework.  J Pharm Sci  2013    PubMed ID: 23450634   DOI: 10.1002/jps.23487
15702  Kuribayashi-Shigetomi K, Onoe H, Takeuchi S.  Cell origami: self-folding of three-dimensional cell-laden microstructures driven by cell traction force  PLoS One  2012  7(12):e51085.  PubMed ID: 23251426   DOI: 10.1371/journal.pone.0051085

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