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Cell No. : Cell Name
RCB2766 : Morris 5123D-TC  update : 2023/10/16
CommentRat (Buffalo) cell line derived from hepatoma. TKG0365 (Deposited from Tohoku Univ.).
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Fukuda, Hiroshi
Originator Kudo, T.
Year of deposit 2008
Original cell TKG0365
Animal _rat < Mammals
Strain name Buffalo
Tissue liver
Classification cancer
History Cell Resource Center for Biomedical Research, Tohoku University(TKG0365)
Lifespan infinite
Morphology epithelial-like
Cellosaurus(Expasy) CVCL_5335
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Culture medium RPMI1640 + 10% FBS
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : once/week, Medium Renewal : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma/Acholeplasma (-)
Animal PCR OK
Isozyme LD, NP, G6PD, AST
Images
deposit info
lot info
Reference information Reference 1
User's Publication 0


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Reference
5263  MORRIS HP, SIDRANSKY H, WAGNER BP, DYER HM.  Some characteristics of transplantable rat hepatoma No. 5123 induced by ingestion of N-(2-fluorenyl) phthalamic acid.  Cancer Res  1960  20:1252-4  PubMed ID: 13772837  

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