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Cell No. : Cell Name
RCB2758 : LAG  update : 2021/05/20
CommentMouse cell line derived from connective tissue. Fibroblast-like. C3H strain. TKG0218 (Deposited from Tohoku Univ.).
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor Fukuda, Hiroshi
Year of deposit 2008
Original cell TKG0218
Animal mouse < Mammals
Strain name C3H
Tissue connective tissue
Classification other
History Cell Resource Center for Biomedical Research, Tohoku University(TKG0218)
Lifespan infinite
Morphology fibroblast-like
Cellosaurus(Expasy) CVCL_B950
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives RPMI1640 + 10% FBS
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
SSLP(mouse) OK
Isozyme LD, NP
deposit info
lot info
Reference information Reference 4
User's Publication 2

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5260  Dei T, Masuko K, Ishii H, Makita T, Tachibana T.  Novel antigen expression on syngeneic somatic cell hybrids of murine spontaneous mammary tumor cells.  J Natl Cancer Inst  1986  77(2):511-9  PubMed ID: 3461212  
5259  Ishikawa S, Tachibana T.  Secondary in vitro generation of CTL specific for MM antigen by stimulation with somatic tumor hybrid, but not with parental tumor cells.  J Immunol  1983  131(4):2059-63  PubMed ID: 6194225  
5261  Dei T, Tachibana T.  Induced resistance in normal mice and growth enhancement in tumor-bearing mice by inoculation with syngeneic hybrid cells.  J Natl Cancer Inst  1980  65(4):739-49  PubMed ID: 6448309   DOI: 10.1093/jnci/65.4.739
5258  Murayama F, Okada Y.  Efficiency of hybrid cell formation from heterokaryons fused by HVI.  Biken J  1970  13(1):1-9  PubMed ID: 4316425  

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User's Publication
14340  Fujiwara T, Yakoub MA, Chandler A, Christ AB, Yang G, Ouerfelli O, Rajasekhar VK, Yoshida A, Kondo H, Hata T, Tazawa H, Dogan Y, Moore MAS, Fujiwara T, Ozaki T, Purdue E, Healey JH.  CSF-1/CSF-1R Signaling Inhibitor Pexidartinib (PLX3397) Reprograms Tumor-Associated Macrophages and Stimulates T-Cell Infiltration in the Sarcoma Microenvironment  Mol Cancer Ther  2021    PubMed ID: 34088832   DOI: 10.1158/1535-7163.MCT-20-0591
15751  Mathew A, Fukuda T, Nagaoka Y, Hasumura T, Morimoto H, Yoshida Y, Maekawa T, Venugopal K, Kumar DS.  Curcumin loaded-PLGA nanoparticles conjugated with Tet-1 peptide for potential use in Alzheimer's disease  PLoS One  2012  7(3):e32616  PubMed ID: 22403681   DOI: 10.1371/journal.pone.0032616

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