Cell No. : Cell Name RCB2745 : A20  update : 2021/01/08
Comment			Mouse cell line derived from lymphoma. Balb/c strain. TKG0571 (Deposited from Tohoku Univ.).
Comment from the depositor
Terms and conditions			There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form			Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use.Please use an appropriate MTA(to see).In case of Restriction "a" or "f", please contact RIKEN BRC(cellbank.brc@riken.jp) regarding any kind of for-profit use.
	Basic information	Depositor	Fukuda, Hiroshi
		Year of deposit	2008
		Original cell	TKG0571
		Animal	mouse < Mammals
		Strain name	BALB/c
		Tissue	lymphoma
		Classification	cancer
		History	Cell Resource Center for Biomedical Research, Tohoku University（TKG0571）
		Lifespan	infinite
		Morphology	lymphocyte-like
			deposit info	lot info
		Medium	Medium List
		Culture type		Suspension cells
		Medium and additives		RPMI1640 + 10% FBS
		Antibiotics		Free
		Passage method		dilution
	Culture information	Passage ratio		1 : 10 split
		SC frequency		Subculture : 2 times/week
		Temperature		37 ℃
		CO2 concentration		5 %
		Freeze medium		Medium + 10% DMSO
		Freezing method		Slow freezing
		Mycoplasma		(-)
		SSLP(mouse)		OK
		Isozyme		LD, NP
Images			deposit info	lot info
Reference information		Reference	4
		User's Publication	2

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Reference

5257  Kim KJ, Finnegan A.  Induction of IgG2a secretion from mIgG2a+ B-lymphoid tumor cells by BCDF present in several antigen-specific T-helper clones.  Cell Immunol  1987  110(1):149-62  PubMed ID: 2960459

5256  Kim KJ, Dejoy SQ.  Con A induction of IgG secretion from a B-lymphoid tumour cell line, A20.  Immunology  1986  59(1):15-21  PubMed ID: 3530982

5255  Laskov R1, Ishay-Michaeli R, Wallach M, Givol D, Kim KJ.  Simultaneous expression of mu- and gamma-chain mRNA in cloned murine B-lymphoma cell lines.  EMBO J  1983  2(2):167-72  PubMed ID: 11894921

5254  Kim KJ, Kanellopoulos-Langevin C, Merwin RM, Sachs DH, Asofsky R.  Establishment and characterization of BALB/c lymphoma lines with B cell properties.  J Immunol  1979  122(2):549-54  PubMed ID: 310843

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User's Publication

12090  Yashiro M, Ohya M, Mima T, Nakashima Y, Kawakami K, Sonou T, Tatsuta K, Yamano Y, Negi S, Shigematsu T.  Excessive ADAM17 activation occurs in uremic patients and may contribute to their immunocompromised status.  Immun Inflamm Dis  2020    PubMed ID: 32180373

11275  Tanaka M, Ishige A, Yaguchi M, Matsumoto T, Shirouzu M, Yokoyama S, Ishikawa F, Kitabayashi I, Takemori T, Harada M.  Development of a simple new flow cytometric antibody-dependent cellular cytotoxicity (ADCC) assay with excellent sensitivity.  J. Immunol. Methods  2019    PubMed ID: 30389576