Cell No. : Cell Name
RCB2745 : A20
update : 2024/12/05
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| Comment | Mouse cell line derived from lymphoma. Balb/c strain. TKG0571 (Deposited from Tohoku Univ.). |
| Comment from the depositor | |
| Terms and conditions | There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC. |
| Remarks | |
| Order Form |
Order Form(C-0005.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
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Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
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Depositor |
Fukuda, Hiroshi
|
| Year of deposit |
2008
|
| Original cell |
TKG0571
|
| Animal |
_mouse
< Mammals
|
| Strain name |
BALB/c
|
| Tissue |
lymphoma
|
| Classification |
cancer
|
| History |
Cell Resource Center for Biomedical Research, Tohoku University（TKG0571）
|
|
Lifespan |
infinite
|
| Morphology |
lymphocyte-like
|
| Cellosaurus(Expasy) |
CVCL_1940
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deposit info |
lot info |
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Medium |
Medium List |
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Culture type |
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Suspension cells
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| Culture method |
|
General Guidelines for Culturing Suspension Cells, 浮遊細胞の培養に関する一般的な注意(Japanese)
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| Culture medium |
|
RPMI1640 + 10% FBS
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| Antibiotics |
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Free
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Passage method |
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dilution
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Culture information
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Passage ratio |
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1 : 10 split
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| SC frequency |
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Subculture : 2 times/week
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| Temperature |
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37
℃
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| CO2 concentration |
|
5
%
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| Freeze medium |
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Medium + 10% DMSO
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| Freezing method |
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Slow freezing
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| Mycoplasma/Acholeplasma |
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(-)
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| SSLP(mouse) |
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OK
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| Isozyme |
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LD, NP
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| Images |
deposit info | lot info |
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| Reference information |
Reference |
4
|
| User's Publication |
3
|
| Reference |
|
5257
Kim KJ, Finnegan A.
Induction of IgG2a secretion from mIgG2a+ B-lymphoid tumor cells by BCDF present in several antigen-specific T-helper clones.
Cell Immunol
1987
110(1):149-62
PubMed ID: 2960459
DOI: 10.1016/0008-8749(87)90109-2
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5256
Kim KJ, Dejoy SQ.
Con A induction of IgG secretion from a B-lymphoid tumour cell line, A20.
Immunology
1986
59(1):15-21
PubMed ID: 3530982
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5255
Laskov R1, Ishay-Michaeli R, Wallach M, Givol D, Kim KJ.
Simultaneous expression of mu- and gamma-chain mRNA in cloned murine B-lymphoma cell lines.
EMBO J
1983
2(2):167-72
PubMed ID: 11894921
DOI: 10.1002/j.1460-2075.1983.tb01400.x
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|
5254
Kim KJ, Kanellopoulos-Langevin C, Merwin RM, Sachs DH, Asofsky R.
Establishment and characterization of BALB/c lymphoma lines with B cell properties.
J Immunol
1979
122(2):549-54
PubMed ID: 310843
|
| User's Publication |
|
20458
Yamaki K, Terashi M, Yamamoto S, Fujiwara R, Inoue JI, Shimizu K, Yanagita S, Doi Y, Kimura KI, Kotani K, Sugihara M, Koyama Y.
Immunoglobulin a suppresses B cell receptor-mediated activation of mouse B cells with differential inhibition of signaling molecules.
Immunopharmacol Immunotoxicol
2022
1-11
PubMed ID: 34844505
DOI: 10.1080/08923973.2021.2006216
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12090
Yashiro M, Ohya M, Mima T, Nakashima Y, Kawakami K, Sonou T, Tatsuta K, Yamano Y, Negi S, Shigematsu T.
Excessive ADAM17 activation occurs in uremic patients and may contribute to their immunocompromised status.
Immun Inflamm Dis
2020
PubMed ID: 32180373
DOI: 10.1002/iid3.298
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11275
Tanaka M, Ishige A, Yaguchi M, Matsumoto T, Shirouzu M, Yokoyama S, Ishikawa F, Kitabayashi I, Takemori T, Harada M.
Development of a simple new flow cytometric antibody-dependent cellular cytotoxicity (ADCC) assay with excellent sensitivity.
J. Immunol. Methods
2019
PubMed ID: 30389576
DOI: 10.1016/j.jim.2018.10.014
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