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Cell No. : Cell Name
RCB2740 : 5A5-WASP  update : 2024/01/17
CommentHybridoma producing monoclonal antibody against WASP (Wiskott-Aldrich syndrome protein). TKG0631 (Deposited from Tohoku Univ.).
Comment from the depositor
Terms and conditionsA prior written permission of the approver(Depositor) is necessary for any kind of use including academic use and for-profit use.
Remarks
approver's address
×
English
Address
Tohoku university

1-1, Seiryou-machi, Aoba-ku, Sendai, 980-8574 Japan
Dr.SASAHARA Yoji
Fax. +81-22-717-7290
Order Form Order Form(C-0005.pdf)   Approval Form(C-0006.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Kumaki, S. ( Fukuda, Hiroshi)
Originator Kawai, Shin
Year of deposit 2008
Original cell TKG0631
Animal _mouse x mouse < Hybrid
Tissue hybridoma
Classification hybrid cells
History Cell Resource Center for Biomedical Research, Tohoku University（TKG0631）
Lifespan infinite
Morphology lymphocyte-like
Cellosaurus(Expasy) CVCL_B990
hybridoma Fusion_cell_1 name spleen cell
Fusion_cell_1 strain mouse, BALB/c
Fusion_cell_2 name SP2/0-Ag14
Fusion_cell_2 strain mouse
Subclass IgG2a
Immunogen human WASP (Wiskott-Aldrich syndrome protein)
deposit info
lot info
Medium Medium List
Culture type Suspension cells
Culture method 浮遊細胞の培養に関する一般的な注意(Japanese)
Culture medium RPMI1640 + 10% FBS
Antibiotics Free
Passage method dilution
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma/Acholeplasma (-)
Isozyme LD, NP
Antibody subclass IgG2a
Images
deposit info
lot info
Reference information Reference 0
User's Publication 0


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