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Cell No. : Cell Name
RCB2726 : KE-F11  update : 2021/05/20
CommentRat cell line derived from malignant schwannoma.
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Yamate, Jyoji
Originator Yamate, Jyoji
Year of deposit 2008
Animal rat < Mammals
Strain name F344/DuCrj
Gender Male
Tissue subcutis of the chest
Disease name Malignant schwannoma.
Classification cancer
Lifespan infinite
Morphology fibroblast-like
Cellosaurus(Expasy) CVCL_1Q77
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives DMEM (low glucose) + 10% FBS
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 8-10 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Animal PCR OK
Images
deposit info
lot info
Reference information Reference 2
User's Publication 0


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Reference
3417  Long-Sheng Chang, Abraham Jacob, Mark Lorenz, Jonathan Rock, Elena M Akhmametyeva, Georgeta Mihai, Petra Schmalbrock, Abhik R Chaudhury, Raul Lopez, Jyoji Yamate, Markus R John, Hannes Wickert, Brian A Neff, Edward Dodson, D Bradley Welling  Growth of benign and malignant schwannoma xenografts in severe combined immunodeficiency mice  Laryngoscope  2006  116(11):2018-26  PubMed ID: 17075413   DOI: 10.1097/01.mlg.0000240185.14224.7d
3416  J Yamate, H Yasui, S J Benn, Y Tsukamoto, M Kuwamura, D Kumagai, S Sakuma, J LaMarre  Characterization of newly established tumor lines from a spontaneous malignant schwannoma in F344 rats: nerve growth factor production, growth inhibition by transforming growth factor-beta1, and macrophage-like phenotype expression  Acta Neuropathol  2003  106(3):221-33  PubMed ID: 12811582   DOI: 10.1007/s00401-003-0723-0

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