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Cell No. : Cell Name
RCB2724 : KB-D8  update : 2023/10/16
CommentRat cell line derived from dendritic cell carcinoma in thymus.
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor Yamate, Jyoji
Originator Yamate, Jyoji
Year of deposit 2008
Animal _rat < Mammals
Strain name F344/DuCrj
Gender Male
Tissue thymus
Disease name dendritic cell sarcoma
Classification cancer
Lifespan infinite
Morphology fibroblast-like
Cellosaurus(Expasy) CVCL_1Q75
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Culture medium DMEM (low glucose) + 10% FBS
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 5 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Animal PCR OK
deposit info
lot info
Reference information Reference 1
User's Publication 0

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3448  Maki Kawashima, Mika Ide, Masako Nakanishi, Mitsuru Kuwamura, Daijiro Kumagai, Jyoji Yamate  Immunophenotypical changes of neoplastic cells and tumor-associated macrophages in a rat dendritic cell sarcoma-derived transplantable tumor line (KB-D8)  Virchows Arch  2003  442(2):141-50  PubMed ID: 12596065   DOI: 10.1007/s00428-002-0716-8

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