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Cell No. : Cell Name
RCB2719 : MT-10  update : 2023/10/16
CommentRat cell line derived from fibrous histiocytoma. After cloning.
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Yamate, Jyoji
Originator Yamate, Jyoji
Year of deposit 2008
Animal _rat < Mammals
Strain name F344/DuCrj
Genus Rattus
Species norvegicus
Gender Male
Age at sampling 15 months
Tissue subcutis of the head
Disease name Malignant Fibrous Histiocytoma
Classification cancer
Lifespan infinite
Morphology fibroblast-like
Cellosaurus(Expasy) CVCL_M802
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Culture medium DMEM (low glucose) + 10% FBS
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 5 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma/Acholeplasma (-)
Animal PCR OK
Images
deposit info
lot info
Reference information Reference 2
User's Publication 0


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Reference
3468  Jyoji Yamate, Takashi Kotera, Mitsuru Kuwamura, Takao Kotani  Potential osteogenic differentiation of cisplatin-resistant rat malignant fibrous histiocytoma-derived cell lines  Exp Toxicol Pathol  2007  58(5):299-309  PubMed ID: 17267196   DOI: 10.1016/j.etp.2006.11.008
1671  Yamate J, Tajima M, Togo M, Shibuya K, Ihara M, Kudow S  Heterogeneity of Cloned Cell Lines Established from a Transplantable Rat Malignamnt Fibrous Histiocytoma  Jpn J Cancer Res  1991  82:298-307  PubMed ID: 1850724   DOI: 10.1111/j.1349-7006.1991.tb01846.x

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