Cell No. : Cell Name
RCB2712 : MAEC
update : 2024/06/07
|
Comment | Vascular endothelial cell line derived from aorta of p53-deficient mouse. |
Comment from the depositor | |
Terms and conditions | 1) In publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature ref. (J Vasc Res 2007;44:138-148) designated by the DEPOSITOR is required. 2) In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. 3) These are no express warrant of merchantability or fitness for a particular purpose, or that the use of the material will not infringe any patent, copyright, trademark, or other proprietary right. |
Remarks | |
Order Form |
Order Form(C-0005.pdf)
 
MTA(C-0007.pdf)
 
MTA(C-0007p.pdf)
 
|
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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|
Basic information
|
Depositor |
Saito, Ichiro
|
Originator |
Nishiyama, Tatsuaki
|
Year of deposit |
2007
|
Animal |
_mouse
< Mammals
|
Gender |
Female
|
Age at sampling |
8 weeks
|
Tissue |
aorta
|
Disease name |
p53-deficient mice
|
Classification |
mutant
|
|
Lifespan |
infinite
|
Morphology |
other
|
Cellosaurus(Expasy) |
CVCL_U411
|
| |
deposit info |
lot info |
|
Medium |
Medium List |
|
Culture type |
|
Adherent cells
|
Culture medium |
|
M199 + 10mM HEPES + 5% FBS + 5ng/ml VEGF+ 1 U/ml Heparin Sodium
|
Antibiotics |
|
Free
|
|
Passage method |
|
0.25% Trypsin
|
Culture information
|
Passage ratio |
|
1 : 8 split
|
SC frequency |
|
Subculture : 2 times/week
|
Temperature |
|
37
℃
|
CO2 concentration |
|
5
%
|
Freeze medium |
|
Medium + 10% DMSO
|
Freezing method |
|
Slow freezing
|
Mycoplasma/Acholeplasma |
|
(-)
|
Animal PCR |
|
OK
|
SSLP(mouse) |
|
OK
|
Reference information |
Reference |
1
|
User's Publication |
0
|