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Cell No. : Cell Name
RCB2712 : MAEC  update : 2022/01/20
CommentVascular endothelial cell line derived from aorta of p53-deficient mouse.
Comment from the depositor
Terms and conditions1) In publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature ref. (J Vasc Res 2007;44:138-148) designated by the DEPOSITOR is required. 2) In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. 3) These are no express warrant of merchantability or fitness for a particular purpose, or that the use of the material will not infringe any patent, copyright, trademark, or other proprietary right.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Saito, Ichiro
Originator Nishiyama, Tatsuaki
Year of deposit 2007
Animal mouse < Mammals
Gender Female
Age at sampling 8 weeks
Tissue aorta
Disease name p53-deficient mice
Classification mutant
Lifespan infinite
Morphology other
Cellosaurus(Expasy) CVCL_U411
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives M199 + 10mM HEPES + 5% FBS + 5ng/ml VEGF+ 1 U/ml Heparin Sodium
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Animal PCR OK
SSLP(mouse) OK
Reference information Reference 1
User's Publication 0


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Reference
3622  Nishiyama T, Mishima K, Ide F, Yamada K, Obara K, Sato A, Hitosugi N, Inoue H, Tsubota K, Saito I.  Functional analysis of an established mouse vascular endothelial cell line.  J Vasc Res  2007  44(2):138-48  PubMed ID: 17215585   DOI: 10.1159/000098520

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