Cell No. : Cell Name RCB2712 : MAEC  update : 2024/06/07
Comment			Vascular endothelial cell line derived from aorta of p53-deficient mouse.
Comment from the depositor
Terms and conditions			1) In publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature ref. (J Vasc Res 2007;44:138-148) designated by the DEPOSITOR is required. 2) In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. 3) These are no express warrant of merchantability or fitness for a particular purpose, or that the use of the material will not infringe any patent, copyright, trademark, or other proprietary right.
Remarks
Order Form			Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)
			Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
	Basic information	Depositor	Saito, Ichiro
		Originator	Nishiyama, Tatsuaki
		Year of deposit	2007
		Animal	_mouse < Mammals
		Gender	Female
		Age at sampling	8 weeks
		Tissue	aorta
		Disease name	p53-deficient mice
		Classification	mutant
		Lifespan	infinite
		Morphology	other
		Cellosaurus(Expasy)	CVCL_U411
			deposit info	lot info
		Medium	Medium List
		Culture type		Adherent cells
		Culture medium		M199 + 10mM HEPES + 5% FBS + 5ng/ml VEGF+ 1 U/ml Heparin Sodium
		Antibiotics		Free
		Passage method		0.25% Trypsin
	Culture information	Passage ratio		1 : 8 split
		SC frequency		Subculture : 2 times/week
		Temperature		37 ℃
		CO2 concentration		5 %
		Freeze medium		Medium + 10% DMSO
		Freezing method		Slow freezing
		Mycoplasma/Acholeplasma		(-)
		Animal PCR		OK
		SSLP(mouse)		OK
Reference information		Reference	1
		User's Publication	1

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Reference

3622  Nishiyama T, Mishima K, Ide F, Yamada K, Obara K, Sato A, Hitosugi N, Inoue H, Tsubota K, Saito I.  Functional analysis of an established mouse vascular endothelial cell line.  J Vasc Res  2007  44(2):138-48  PubMed ID: 17215585   DOI: 10.1159/000098520

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User's Publication

22162  Tsukiji N, Yokomori R, Takusagawa K, Shirai T, Oishi S, Sasaki T, Takano K, Suzuki-Inoue K.  C-type lectin-like receptor-2 in platelets mediates ferric chloride-induced platelet activation and attenuates ferroptosis of endothelial cells.  J Thromb Haemost  2024  22(6):1749-1757  PubMed ID: 38811291   DOI: 10.1016/j.jtha.2024.03.003