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細胞番号 : 細胞名
RCB2710 : Atg5^(+/+)MEF  update : 2023/10/16
細胞特性(Comment:英)Mouse embryonic fibroblasts. Wild type control for RCB2711.
細胞特性(日)マウス胎児由来線維芽細胞。RCB2711の比較対照野生型細胞。
細胞特性(寄託者記述:英)
細胞特性(寄託者記述:日)
使用条件(英)1) BIOLOGICAL RESOURCE is limited to for academic researches in non-profit organization. 2) The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. 3) Any use of the BIOLOGICAL RESOURCE for profit purposes by a non-profit organization or any use of the BIOLOGICAL RESOURCE by a profit organization requires a separate license from the DEPOSITOR prior to distribution. 4) In publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the original publication (Nature 2004;432;1032-1036) designated by the DEPOSITOR is required. 5) In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. 6) The RECIPIENT must contact the DEPOSITOR in case of application for any patents or commercial use with the results from the use of BIOLOGICAL RESOURCE.
使用条件(日)1) 非営利団体による学術研究に限る。 2) 利用者は事前に寄託者の提供承諾書を得ること。 3) 当該リソースの非営利団体の営利目的での利用および営利団体の利用については、事前に 寄託者との間で、別途使用許諾に係る契約の締結が必要である。 4) 研究成果の公表にあたって寄託者の指定する文献 (Nature 2004;432;1032-1036) を引用すること。 5) 研究成果の公表にあたって謝辞の表明を必要とする。 6) 利用者が本件リソースを使用して得られた研究成果に基づき特許等の申請、及び事業活動を行う場合は、寄託者と別途協議を行う。
備考(英)
備考(日)
承諾者住所
×
Japanese
住所
113-0033 東京都文京区本郷7-3-1
東京大学   大学院医学系研究科 分子生物学分野 水島研究室
水島 昇 先生
Fax. 03-3815-1490

English
Address
The University of Tokyo, Graduate School of Medicine
Department of Biochemistry and Molecular Biology,Mizushima Lab.
7-3-1, Hongo, Bunkyo-ku, Tokyo, 113-0033 Japan
Dr.MIZUSHIMA Noboru
Fax. +81-3-3815-1490
提供申込書類(英) Order Form(C-0005.pdf)   Approval Form(C-0006.pdf)   MTA(C-0007.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
提供申込書類(日) 依頼書C-0001.pdf   承諾書C-0002.pdf   同意書(非営利学術目的)C-0003.pdf  
提供同意書は、使用機関の種類や目的に応じて、非営利学術目的 (C-XXXX) と営利目的 (C-XXXXp) の2種類があります。該当する提供同意書をご使用ください(詳細)。特許等の取得及び商業利用等は事前に必ず cellbank.brc@riken.jp までご連絡ください。
提供手数料 手数料とお支払いについてはこちらをご覧ください。
細胞基本情報 寄託者 Mizushima, Noboru
樹立者 Mizushima, Noboru
寄託日 2007
別名 Atg5WT_A
動物種 _mouse < Mammals
動物系統名 C57BL/6x129sv
性別 Unknown
embryo
採取組織 embryo/fetus, whole
病名 wild type (ATG gene +/+)
細胞分類 transformed
遺伝子改変 recombinant
外来遺伝子 SV40 large T
細胞寿命 infinite
細胞形態 fibroblast-like
Cellosaurus(Expasy) CVCL_0J74
細胞培養・検査情報
寄託時情報
ロット情報
培地・試薬情報 培地・試薬一覧はこちらをご覧ください。
培養形態 Adherent cells
培地 DMEM (low glucose) + 10% FBS
抗生物質 Free
継代方法 0.25% Trypsin
継代密度 1 : 6-10 split
継代・培地交換頻度 Subculture or Medium Renewal : once/2-3 days
培養最適温度 37 ℃
二酸化炭素濃度 5 %
凍結培地 Medium + 10% DMSO
凍結方法 Slow freezing
マイコプラズマ (-)
マウス系統検査 OK
アイソザイム検査 LD, NP
画像情報
寄託時情報
ロット情報
文献情報 Reference(英) 2件
Reference(日) 0件
利用者成果(英) 43件
利用者成果(日) 0件

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Reference(英)
8452  Mizushima N, Yoshimori T, Levine B.  Methods in mammalian autophagy research  Cell  2010  140(3):313-26  PubMed ID: 20144757   DOI: 10.1016/j.cell.2010.01.028
3621  Kuma A, Hatano M, Matsui M, Yamamoto A, Nakaya H, Yoshimori T, Ohsumi Y, Tokuhisa T, Mizushima N.  The role of autophagy during the early neonatal starvation period.  Nature  2004  432(7020):1032-6  PubMed ID: 15525940   DOI: 10.1038/nature03029

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利用者成果(英)
17739  Hung CH, Yen JB, Chang PJ, Chen LW, Huang TY, Tsai WJ, Tsai YC.  Characterization of Human Norovirus Nonstructural Protein NS1.2 Involved in the Induction of the Filamentous Endoplasmic Reticulum, Enlarged Lipid Droplets, LC3 Recruitment, and Interaction with NTPase and NS4  Viruses  2023  15(3):812  PubMed ID: 36992520   DOI: 10.3390/v15030812
19944  Aihara S, Torisu K, Uchida Y, Imazu N, Nakano T, Kitazono T.  Spermidine from arginine metabolism activates Nrf2 and inhibits kidney fibrosis  Commun Biol  2023  6(1):676  PubMed ID: 37380734   DOI: 10.1038/s42003-023-05057-w
21118  Aki T, Kukita M, Takata M, Funakoshi T, Unuma K, Uemura K.  Induction of filamin-C and its involvement in the regulation of cellular senescence and apoptosis in Huh-7 hepatoma cells during arsenic trioxide exposure.  Biochem Biophys Res Commun  2023  651:92-97  PubMed ID: 36801614   DOI: 10.1016/j.bbrc.2023.02.020
20691  Kajiwara K, Osaki H, Greßies S, Kuwata K, Kim JH, Gensch T, Sato Y, Glorius F, Yamaguchi S, Taki M.  A negative-solvatochromic fluorescent probe for visualizing intracellular distributions of fatty acid metabolites.  Nat Commun  2022  13(1):2533  PubMed ID: 35534485   DOI: 10.1038/s41467-022-30153-6
18096  Qiao Y, Choi JE, Tien JC, Simko SA, Rajendiran T, Vo JN, Delekta AD, Wang L, Xiao L, Hodge NB, Desai P, Mendoza S, Juckette K, Xu A, Soni T, Su F, Wang R, Cao X, Yu J, Kryczek I, Wang XM, Wang X, Siddiqui J, Wang Z, Bernard A, Fernandez-Salas E, Navone NM, Ellison SJ, Ding K, Eskelinen EL, Heath EI, Klionsky DJ, Zou W, Chinnaiyan AM.  Autophagy Inhibition by Targeting PIKfyve Potentiates Response to Immune Checkpoint Blockade in Prostate Cancer  Nat Cancer  2021  2:978-993  PubMed ID: 34738088   DOI: 10.1038/s43018-021-00237-1
20339  Sharma KB, Chhabra S, Aggarwal S, Tripathi A, Banerjee A, Yadav AK, Vrati S, Kalia M.  Proteomic landscape of Japanese encephalitis virus-infected fibroblasts.  J Gen Virol  2021    PubMed ID: 34546869   DOI: 10.1099/jgv.0.001657
16432  Sarkar R, Sharma KB, Kumari A, Asthana S, Kalia M.  Japanese encephalitis virus capsid protein interacts with non-lipidated MAP1LC3 on replication membranes and lipid droplets  J Gen Virol  2021  Jan;102(1)  PubMed ID: 33095129   DOI: 10.1099/jgv.0.001508
16981  Uchida Y, Torisu K, Ueki K, Tsuruya K, Nakano T, Kitazono T.  Autophagy gene ATG7 regulates albumin transcytosis in renal tubule epithelial cells  Am J Physiol Renal Physiol  2021  321(5):F572-F586  PubMed ID: 34541900   DOI: 10.1152/ajprenal.00172.2021
11865  Xu ZD, Wang Y, Liang G, Liu ZQ, Ma WH, Chu CT, Wei HF.  Propofol affects mouse embryonic fibroblast survival and proliferation in vitro via ATG5- and calcium-dependent regulation of autophagy.  Acta Pharmacol. Sin.  2020  41(3):303-310  PubMed ID: 31645660   DOI: 10.1038/s41401-019-0303-z
13223  Liu EA, Schultz ML, Mochida C, Chung C, Paulson HL, Lieberman AP.  Fbxo2 mediates clearance of damaged lysosomes and modifies neurodegeneration in the Niemann-Pick C brain.  JCI Insight  2020    PubMed ID: 32931479   DOI: 10.1172/jci.insight.136676
15153  Hitomi K, Okada R, Loo TM, Miyata K, Nakamura AJ, Takahashi A.  DNA Damage Regulates Senescence-Associated Extracellular Vesicle Release via the Ceramide Pathway to Prevent Excessive Inflammatory Responses  Int J Mol Sci  2020  21(10):3720  PubMed ID: 32466233   DOI: 10.3390/ijms21103720
11371  Aki T, Unuma K, Noritake K, Hirayama N, Funakoshi T, Uemura K.  Formation of high molecular weight p62 by CORM-3.  PLoS ONE  2019    PubMed ID: 30620762   DOI: 10.1371/journal.pone.0210474
11463  Iwasawa T, Shinomiya T, Ota N, Shibata N, Nakata K, Shiina I, Nagahara Y.  Novel Ridaifen-B Structure Analog Induces Apoptosis and Autophagy Depending on Pyrrolidine Side Chain.  Biol. Pharm. Bull.  2019    PubMed ID: 30828072   DOI: 10.1248/bpb.b18-00643
11727  Ji CH, Kim HY, Heo AJ, Lee SH, Lee MJ, Kim SB, Srinivasrao G, Mun SR, Cha-Molstad H, Ciechanover A, Choi CY, Lee HG, Kim BY, Kwon YT.  The N-Degron Pathway Mediates ER-phagy.  Mol. Cell  2019    PubMed ID: 31375263   DOI: 10.1016/j.molcel.2019.06.028
6908  Ko PJ, Woodrow C, Dubreuil MM, Martin BR, Skouta R, Bassik MC, Dixon S.  A ZDHHC5-GOLGA7 Protein Acyltransferase Complex Promotes Nonapoptotic Cell Death.  Cell Chem Biol  2019  26(12):1716-1724  PubMed ID: 31631010   DOI: 10.1016/j.chembiol.2019.09.014
9181  Huiyin Lan, Mingjia Tan, Qiang Zhang, Fei Yang, Siyuan Wang, Hua Li, Xiufang Xiong, Yi Sun  LSD1 Destabilizes FBXW7 and Abrogates FBXW7 Functions Independent of Its Demethylase Activity  Proc Natl Acad Sci U S A  2019  116 (25), 12311-12320  PubMed ID: 31152129   DOI: 10.1073/pnas.1902012116
11079  Sharma M, Sharma KB, Chauhan S, Bhattacharyya S, Vrati S, Kalia M.  Diphenyleneiodonium enhances oxidative stress and inhibits Japanese encephalitis virus induced autophagy and ER stress pathways.  Biochem. Biophys. Res. Commun.  2018  502(2):232-237  PubMed ID: 29792860   DOI: 10.1016/j.bbrc.2018.05.149
4148  Aki T, Unuma K, Noritake K, Kurahashi H, Funakoshi T, Uemura K.  Interaction of carbon monoxide-releasing ruthenium carbonyl CORM-3 with plasma fibronectin.  Toxicol In Vitro  2018    PubMed ID: 29580987   DOI: 10.1016/j.tiv.2018.03.010
9173  Alexa R Wilden, Joshua A Molina, Melissa Feuerborn, Daniel Boyle, Stella Y Lee  Glutamine-dependent Lysosome Homeostatic Changes Induced by Starvation and Lysosome Inhibition  Biochim Biophys Acta Mol Cell Res  2018  1865 (9), 1356-1367  PubMed ID: 29966622   DOI: 10.1016/j.bbamcr.2018.06.014
15047  Real E, Rodrigues L, Cabal GG, Enguita FJ, Mancio-Silva L, Mello-Vieira J, Beatty W, Vera IM, Zuzarte-Luís V, Figueira TN, Mair GR, Mota MM.  Plasmodium UIS3 sequesters host LC3 to avoid elimination by autophagy in hepatocytes  Nat Microbiol  2018  3(1):17-25  PubMed ID: 29109477   DOI: 10.1038/s41564-017-0054-x
9185  Hyunjoo Cha-Molstad, Ji Eun Yu, Zhiwei Feng, Su Hyun Lee, Jung Gi Kim, Peng Yang, Bitnara Han, Ki Woon Sung, Young Dong Yoo, Joonsung Hwang, Terry McGuire, Sang Mi Shim, Hyun Dong Song, Srinivasrao Ganipisetti 1, Nuozhou Wang, Jun Min Jang, Min Jae Lee, Seung Jun Kim, Kyung Ho Lee 1, Jin Tae Hong, Aaron Ciechanover , Inhee Mook-Jung, Kwang Pyo Kim, Xiang-Qun Xie, Yong Tae Kwon, Bo Yeon Kim  p62/SQSTM1/Sequestosome-1 Is an N-recognin of the N-end Rule Pathway Which Modulates Autophagosome Biogenesis  Nat Commun  2017  8 (1), 102  PubMed ID: 28740232   DOI: 10.1038/s41467-017-00085-7
14213  Into T, Horie T, Inomata M, Gohda J, Inoue JI, Murakami Y, Niida S.  Basal autophagy prevents autoactivation or enhancement of inflammatory signals by targeting monomeric MyD88  Sci Rep  2017  7(1):1009  PubMed ID: 28432355   DOI: 10.1038/s41598-017-01246-w
14756  Kawano S, Torisu T, Esaki M, Torisu K, Matsuno Y, Kitazono T.  Autophagy promotes degradation of internalized collagen and regulates distribution of focal adhesions to suppress cell adhesion  Biol Open  2017  6(11):1644-1653  PubMed ID: 28970230   DOI: 10.1242/bio.027458
9158  Nguyen T Tien, Ilker Karaca , Irfan Y Tamboli, Jochen Walter  Trehalose Alters Subcellular Trafficking and the Metabolism of the Alzheimer-associated Amyloid Precursor Protein  J Biol Chem  2016  291 (20), 10528-40  PubMed ID: 26957541   DOI: 10.1074/jbc.M116.719286
9164  Bhavya B Chandrika, Cheng Yang, Yang Ou, Xiaoke Feng, Djamali Muhoza, Alexandrea F Holmes, Sue Theus, Sarika Deshmukh, Randy S Haun, Gur P Kaushal  Endoplasmic Reticulum Stress-Induced Autophagy Provides Cytoprotection From Chemical Hypoxia and Oxidant Injury and Ameliorates Renal Ischemia-Reperfusion Injury  PLoS One   2015  10 (10), e0140025  PubMed ID: 26444017   DOI: 10.1371/journal.pone.0140025
15041  Cha-Molstad H, Sung KS, Hwang J, Kim KA, Yu JE, Yoo YD, Jang JM, Han DH, Molstad M, Kim JG, Lee YJ, Zakrzewska A, Kim SH, Kim ST, Kim SY, Lee HG, Soung NK, Ahn JS, Ciechanover A, Kim BY, Kwon YT.  Amino-terminal arginylation targets endoplasmic reticulum chaperone BiP for autophagy through p62 binding  Nat Cell Biol  2015  17(7):917-29  PubMed ID: 26075355   DOI: 10.1038/ncb3177
15211  Unuma K, Aki T, Funakoshi T, Hashimoto K, Uemura K.  Extrusion of mitochondrial contents from lipopolysaccharide-stimulated cells: Involvement of autophagy  Autophagy  2015  11(9):1520-36  PubMed ID: 26102061   DOI: 10.1080/15548627.2015.1063765
9111  Shintaro Kobayashi, Yasuko Orba, Hiroki Yamaguchi, Kenta Takahashi, Michihito Sasaki, Rie Hasebe, Takashi Kimura, Hirofumi Sawa  Autophagy Inhibits Viral Genome Replication and Gene Expression Stages in West Nile Virus Infection  Virus Res  2014  191, 83-91  PubMed ID: 25091564   DOI: 10.1016/j.virusres.2014.07.016
9137  Isabelle Hamer, Emeline Goffin, Xavier De Bolle, Jean-Jacques Letesson, Michel Jadot  Replication of Brucella Abortus and Brucella Melitensis in Fibroblasts Does Not Require Atg5-dependent Macroautophagy  BMC Microbiol   2014  14, 223  PubMed ID: 25179110   DOI: 10.1186/s12866-014-0223-5
9184  Bo-Jhih Guan, Dawid Krokowski, Mithu Majumder, Christine L Schmotzer, Scot R Kimball, William C Merrick, Antonis E Koromilas, Maria Hatzoglou  Translational Control During Endoplasmic Reticulum Stress Beyond Phosphorylation of the Translation Initiation Factor eIF2α  J Biol Chem  2014  289 (18), 12593-611  PubMed ID: 24648524   DOI: 10.1074/jbc.M113.543215
15871  McLelland GL, Soubannier V, Chen CX, McBride HM, Fon EA.  Parkin and PINK1 function in a vesicular trafficking pathway regulating mitochondrial quality control  EMBO J   2014  33(4):282-95  PubMed ID: 24446486   DOI: 10.1002/embj.201385902
16009  Sharma M, Bhattacharyya S, Nain M, Kaur M, Sood V, Gupta V, Khasa R, Abdin MZ, Vrati S, Kalia M.  Japanese encephalitis virus replication is negatively regulated by autophagy and occurs on LC3-I- and EDEM1-containing membranes  Autophagy  2014  10(9):1637-51  PubMed ID: 25046112   DOI: 10.4161/auto.29455
9165  Hiroko Inoue, Katsunori Hase, Arata Segawa, Tsuyoshi Takita  H89 (N-[2-p-bromocinnamylamino-ethyl]-5-isoquinolinesulphonamide) Induces Autophagy Independently of Protein Kinase A Inhibition  Eur J Pharmacol   2013  714 (1-3), 170-7  PubMed ID: 23810683   DOI: 10.1016/j.ejphar.2013.06.018
9168  Shintaro Kobayashi, Tadaki Suzuki, Manabu Igarashi, Yasuko Orba, Noriko Ohtake, Keita Nagakawa, Kenichi Niikura, Takashi Kimura, Harumi Kasamatsu, Hirofumi Sawa  Cysteine Residues in the Major Capsid Protein, Vp1, of the JC Virus Are Important for Protein Stability and Oligomer Formation  PLoS One  2013  8 (10), e76668  PubMed ID: 24130786   DOI: 10.1371/journal.pone.0076668
9150  Yutaka Ueda, Fan-Yan Wei, Taku-ichiro Hide, Hiroyuki Michiue, Kentaro Takayama, Taku Kaitsuka, Hideo Nakamura, Keishi Makino, Jun-ichi Kuratsu, Shiroh Futaki, Kazuhito Tomizawa  Induction of Autophagic Cell Death of Glioma-Initiating Cells by Cell-Penetrating D-isomer Peptides Consisting of Pas and the p53 C-terminus  Biomaterials  2012  33 (35), 9061-9  PubMed ID: 23006589   DOI: 10.1016/j.biomaterials.2012.09.003
9153  Satoshi Tsuyuki, Sayuri Fukui, Akari Watanabe, Satoshi Akune, Mizuki Tanabe, Kenichi Yoshida  Delphinidin Induces Autolysosome as Well as Autophagosome Formation and Delphinidin-Induced Autophagy Exerts a Cell Protective Role  J Biochem Mol Toxicol  2012  26 (11), 445-53  PubMed ID: 23129091   DOI: 10.1002/jbt.21443
9154  Kai Er Eng, Marc D Panas, Deirdre Murphy, Gunilla B Karlsson Hedestam, Gerald M McInerney  Accumulation of Autophagosomes in Semliki Forest Virus-Infected Cells Is Dependent on Expression of the Viral Glycoproteins  J Virol  2012  86 (10), 5674-85  PubMed ID: 22438538   DOI: 10.1128/JVI.06581-11
7903  Tian Y, Bustos V, Flajolet M, Greengard P.  A small-molecule enhancer of autophagy decreases levels of Abeta and APP-CTF via Atg5-dependent autophagy pathway.  FASEB J.  2011  25:1934-42  PubMed ID: 21368103   DOI: 10.1096/fj.10-175158
7963  Tamboli IY, Hampel H, Tien NT, Tolksdorf K, Breiden B, Mathews PM, Saftig P, Sandhoff K, Walter J.  Sphingolipid storage affects autophagic metabolism of the amyloid precursor protein and promotes Abeta generation.  J. Neurosci.  2011  31:1837-49  PubMed ID: 21289194   DOI: 10.1523/JNEUROSCI.2954-10.2011
9148  Chengqun Huang, Allen M Andres, Eric P Ratliff, Genaro Hernandez, Pamela Lee, Roberta A Gottlieb  Preconditioning Involves Selective Mitophagy Mediated by Parkin and p62/SQSTM1  PLoS One  2011  6 (6), e20975  PubMed ID: 21687634   DOI: 10.1371/journal.pone.0020975
9152  Natura Myeku, Maria E Figueiredo-Pereira  Dynamics of the Degradation of Ubiquitinated Proteins by Proteasomes and Autophagy: Association With Sequestosome 1/p62  J Biol Chem  2011  286 (25), 22426-40  PubMed ID: 21536669   DOI: 10.1074/jbc.M110.149252
9163  Amy Colleran 1, Aideen Ryan, Angela O'Gorman, Coralie Mureau, Catherine Liptrot, Peter Dockery, Howard Fearnhead, Laurence J Egan  Autophagosomal IkappaB Alpha Degradation Plays a Role in the Long Term Control of Tumor Necrosis Factor-Alpha-Induced Nuclear factor-kappaB (NF-kappaB) Activity  J Biol Chem  2011  286 (26), 22886-93  PubMed ID: 21454695   DOI: 10.1074/jbc.M110.199950
8269  Eng KE, Panas MD, Karlsson Hedestam GB, McInerney GM.  A novel quantitative flow cytometry-based assay for autophagy.  Autophagy  2010  6:634-41  PubMed ID: 20458170   DOI: 10.4161/auto.6.5.12112

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