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Cell No. : Cell Name
RCB2710 : Atg5^(+/+)MEF  update : 2022/07/08
CommentMouse embryonic fibroblasts. Wild type control for RCB2711.
Comment from the depositor
Terms and conditions1) BIOLOGICAL RESOURCE is limited to for academic researches in non-profit organization. 2) The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. 3) Any use of the BIOLOGICAL RESOURCE for profit purposes by a non-profit organization or any use of the BIOLOGICAL RESOURCE by a profit organization requires a separate license from the DEPOSITOR prior to distribution. 4) In publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the original publication (Nature 2004;432;1032-1036) designated by the DEPOSITOR is required. 5) In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. 6) The RECIPIENT must contact the DEPOSITOR in case of application for any patents or commercial use with the results from the use of BIOLOGICAL RESOURCE.
approver's address
The University of Tokyo, Graduate School of Medicine
Department of Biochemistry and Molecular Biology,Mizushima Lab.
7-3-1, Hongo, Bunkyo-ku, Tokyo, 113-0033 Japan
Fax. +81-3-3815-1490
Order Form Order Form(C-0005.pdf)   Approval Form(C-0006.pdf)   MTA(C-0007.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor Mizushima, Noboru
Originator Mizushima, Noboru
Year of deposit 2007
Another name Atg5WT_A
Animal mouse < Mammals
Strain name C57BL/6x129sv
Gender Unknown
Age at sampling embryo
Tissue embryo/fetus, whole
Disease name wild type (ATG gene +/+)
Classification transformed
Recombinant recombinant
Exogene SV40 large T
Lifespan infinite
Morphology fibroblast-like
Cellosaurus(Expasy) CVCL_0J74
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives DMEM (low glucose) + 10% FBS
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 6-10 split
SC frequency Subculture or Medium Renewal : once/2-3 days
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
SSLP(mouse) OK
Isozyme LD, NP
deposit info
lot info
Reference information Reference 2
User's Publication 39

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8452  Mizushima N, Yoshimori T, Levine B.  Methods in mammalian autophagy research  Cell  2010  140(3):313-26  PubMed ID: 20144757   DOI: 10.1016/j.cell.2010.01.028
3621  Kuma A, Hatano M, Matsui M, Yamamoto A, Nakaya H, Yoshimori T, Ohsumi Y, Tokuhisa T, Mizushima N.  The role of autophagy during the early neonatal starvation period.  Nature  2004  432(7020):1032-6  PubMed ID: 15525940   DOI: 10.1038/nature03029

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User's Publication
20691  Kajiwara K, Osaki H, Greßies S, Kuwata K, Kim JH, Gensch T, Sato Y, Glorius F, Yamaguchi S, Taki M.  A negative-solvatochromic fluorescent probe for visualizing intracellular distributions of fatty acid metabolites.  Nat Commun  2022  13(1):2533  PubMed ID: 35534485   DOI: 10.1038/s41467-022-30153-6
20339  Sharma KB, Chhabra S, Aggarwal S, Tripathi A, Banerjee A, Yadav AK, Vrati S, Kalia M.  Proteomic landscape of Japanese encephalitis virus-infected fibroblasts.  J Gen Virol  2021    PubMed ID: 34546869   DOI: 10.1099/jgv.0.001657
16432  Sarkar R, Sharma KB, Kumari A, Asthana S, Kalia M.  Japanese encephalitis virus capsid protein interacts with non-lipidated MAP1LC3 on replication membranes and lipid droplets  J Gen Virol  2021  Jan;102(1)  PubMed ID: 33095129   DOI: 10.1099/jgv.0.001508
16981  Uchida Y, Torisu K, Ueki K, Tsuruya K, Nakano T, Kitazono T.  Autophagy gene ATG7 regulates albumin transcytosis in renal tubule epithelial cells  Am J Physiol Renal Physiol  2021  321(5):F572-F586  PubMed ID: 34541900   DOI: 10.1152/ajprenal.00172.2021
11865  Xu ZD, Wang Y, Liang G, Liu ZQ, Ma WH, Chu CT, Wei HF.  Propofol affects mouse embryonic fibroblast survival and proliferation in vitro via ATG5- and calcium-dependent regulation of autophagy.  Acta Pharmacol. Sin.  2020  41(3):303-310  PubMed ID: 31645660   DOI: 10.1038/s41401-019-0303-z
13223  Liu EA, Schultz ML, Mochida C, Chung C, Paulson HL, Lieberman AP.  Fbxo2 mediates clearance of damaged lysosomes and modifies neurodegeneration in the Niemann-Pick C brain.  JCI Insight  2020    PubMed ID: 32931479   DOI: 10.1172/jci.insight.136676
15153  Hitomi K, Okada R, Loo TM, Miyata K, Nakamura AJ, Takahashi A.  DNA Damage Regulates Senescence-Associated Extracellular Vesicle Release via the Ceramide Pathway to Prevent Excessive Inflammatory Responses  Int J Mol Sci  2020  21(10):3720  PubMed ID: 32466233   DOI: 10.3390/ijms21103720
11371  Aki T, Unuma K, Noritake K, Hirayama N, Funakoshi T, Uemura K.  Formation of high molecular weight p62 by CORM-3.  PLoS ONE  2019    PubMed ID: 30620762   DOI: 10.1371/journal.pone.0210474
11463  Iwasawa T, Shinomiya T, Ota N, Shibata N, Nakata K, Shiina I, Nagahara Y.  Novel Ridaifen-B Structure Analog Induces Apoptosis and Autophagy Depending on Pyrrolidine Side Chain.  Biol. Pharm. Bull.  2019    PubMed ID: 30828072   DOI: 10.1248/bpb.b18-00643
11727  Ji CH, Kim HY, Heo AJ, Lee SH, Lee MJ, Kim SB, Srinivasrao G, Mun SR, Cha-Molstad H, Ciechanover A, Choi CY, Lee HG, Kim BY, Kwon YT.  The N-Degron Pathway Mediates ER-phagy.  Mol. Cell  2019    PubMed ID: 31375263   DOI: 10.1016/j.molcel.2019.06.028
6908  Ko PJ, Woodrow C, Dubreuil MM, Martin BR, Skouta R, Bassik MC, Dixon S.  A ZDHHC5-GOLGA7 Protein Acyltransferase Complex Promotes Nonapoptotic Cell Death.  Cell Chem Biol  2019  26(12):1716-1724  PubMed ID: 31631010   DOI: 10.1016/j.chembiol.2019.09.014
9181  Huiyin Lan, Mingjia Tan, Qiang Zhang, Fei Yang, Siyuan Wang, Hua Li, Xiufang Xiong, Yi Sun  LSD1 Destabilizes FBXW7 and Abrogates FBXW7 Functions Independent of Its Demethylase Activity  Proc Natl Acad Sci U S A  2019  116 (25), 12311-12320  PubMed ID: 31152129   DOI: 10.1073/pnas.1902012116
11079  Sharma M, Sharma KB, Chauhan S, Bhattacharyya S, Vrati S, Kalia M.  Diphenyleneiodonium enhances oxidative stress and inhibits Japanese encephalitis virus induced autophagy and ER stress pathways.  Biochem. Biophys. Res. Commun.  2018  502(2):232-237  PubMed ID: 29792860   DOI: 10.1016/j.bbrc.2018.05.149
4148  Aki T, Unuma K, Noritake K, Kurahashi H, Funakoshi T, Uemura K.  Interaction of carbon monoxide-releasing ruthenium carbonyl CORM-3 with plasma fibronectin.  Toxicol In Vitro  2018    PubMed ID: 29580987   DOI: 10.1016/j.tiv.2018.03.010
9173  Alexa R Wilden, Joshua A Molina, Melissa Feuerborn, Daniel Boyle, Stella Y Lee  Glutamine-dependent Lysosome Homeostatic Changes Induced by Starvation and Lysosome Inhibition  Biochim Biophys Acta Mol Cell Res  2018  1865 (9), 1356-1367  PubMed ID: 29966622   DOI: 10.1016/j.bbamcr.2018.06.014
15047  Real E, Rodrigues L, Cabal GG, Enguita FJ, Mancio-Silva L, Mello-Vieira J, Beatty W, Vera IM, Zuzarte-Luís V, Figueira TN, Mair GR, Mota MM.  Plasmodium UIS3 sequesters host LC3 to avoid elimination by autophagy in hepatocytes  Nat Microbiol  2018  3(1):17-25  PubMed ID: 29109477   DOI: 10.1038/s41564-017-0054-x
9185  Hyunjoo Cha-Molstad, Ji Eun Yu, Zhiwei Feng, Su Hyun Lee, Jung Gi Kim, Peng Yang, Bitnara Han, Ki Woon Sung, Young Dong Yoo, Joonsung Hwang, Terry McGuire, Sang Mi Shim, Hyun Dong Song, Srinivasrao Ganipisetti 1, Nuozhou Wang, Jun Min Jang, Min Jae Lee, Seung Jun Kim, Kyung Ho Lee 1, Jin Tae Hong, Aaron Ciechanover , Inhee Mook-Jung, Kwang Pyo Kim, Xiang-Qun Xie, Yong Tae Kwon, Bo Yeon Kim  p62/SQSTM1/Sequestosome-1 Is an N-recognin of the N-end Rule Pathway Which Modulates Autophagosome Biogenesis  Nat Commun  2017  8 (1), 102  PubMed ID: 28740232   DOI: 10.1038/s41467-017-00085-7
14213  Into T, Horie T, Inomata M, Gohda J, Inoue JI, Murakami Y, Niida S.  Basal autophagy prevents autoactivation or enhancement of inflammatory signals by targeting monomeric MyD88  Sci Rep  2017  7(1):1009  PubMed ID: 28432355   DOI: 10.1038/s41598-017-01246-w
14756  Kawano S, Torisu T, Esaki M, Torisu K, Matsuno Y, Kitazono T.  Autophagy promotes degradation of internalized collagen and regulates distribution of focal adhesions to suppress cell adhesion  Biol Open  2017  6(11):1644-1653  PubMed ID: 28970230   DOI: 10.1242/bio.027458
9158  Nguyen T Tien, Ilker Karaca , Irfan Y Tamboli, Jochen Walter  Trehalose Alters Subcellular Trafficking and the Metabolism of the Alzheimer-associated Amyloid Precursor Protein  J Biol Chem  2016  291 (20), 10528-40  PubMed ID: 26957541   DOI: 10.1074/jbc.M116.719286
9164  Bhavya B Chandrika, Cheng Yang, Yang Ou, Xiaoke Feng, Djamali Muhoza, Alexandrea F Holmes, Sue Theus, Sarika Deshmukh, Randy S Haun, Gur P Kaushal  Endoplasmic Reticulum Stress-Induced Autophagy Provides Cytoprotection From Chemical Hypoxia and Oxidant Injury and Ameliorates Renal Ischemia-Reperfusion Injury  PLoS One   2015  10 (10), e0140025  PubMed ID: 26444017   DOI: 10.1371/journal.pone.0140025
15041  Cha-Molstad H, Sung KS, Hwang J, Kim KA, Yu JE, Yoo YD, Jang JM, Han DH, Molstad M, Kim JG, Lee YJ, Zakrzewska A, Kim SH, Kim ST, Kim SY, Lee HG, Soung NK, Ahn JS, Ciechanover A, Kim BY, Kwon YT.  Amino-terminal arginylation targets endoplasmic reticulum chaperone BiP for autophagy through p62 binding  Nat Cell Biol  2015  17(7):917-29  PubMed ID: 26075355   DOI: 10.1038/ncb3177
15211  Unuma K, Aki T, Funakoshi T, Hashimoto K, Uemura K.  Extrusion of mitochondrial contents from lipopolysaccharide-stimulated cells: Involvement of autophagy  Autophagy  2015  11(9):1520-36  PubMed ID: 26102061   DOI: 10.1080/15548627.2015.1063765
9111  Shintaro Kobayashi, Yasuko Orba, Hiroki Yamaguchi, Kenta Takahashi, Michihito Sasaki, Rie Hasebe, Takashi Kimura, Hirofumi Sawa  Autophagy Inhibits Viral Genome Replication and Gene Expression Stages in West Nile Virus Infection  Virus Res  2014  191, 83-91  PubMed ID: 25091564   DOI: 10.1016/j.virusres.2014.07.016
9137  Isabelle Hamer, Emeline Goffin, Xavier De Bolle, Jean-Jacques Letesson, Michel Jadot  Replication of Brucella Abortus and Brucella Melitensis in Fibroblasts Does Not Require Atg5-dependent Macroautophagy  BMC Microbiol   2014  14, 223  PubMed ID: 25179110   DOI: 10.1186/s12866-014-0223-5
9184  Bo-Jhih Guan, Dawid Krokowski, Mithu Majumder, Christine L Schmotzer, Scot R Kimball, William C Merrick, Antonis E Koromilas, Maria Hatzoglou  Translational Control During Endoplasmic Reticulum Stress Beyond Phosphorylation of the Translation Initiation Factor eIF2α  J Biol Chem  2014  289 (18), 12593-611  PubMed ID: 24648524   DOI: 10.1074/jbc.M113.543215
15871  McLelland GL, Soubannier V, Chen CX, McBride HM, Fon EA.  Parkin and PINK1 function in a vesicular trafficking pathway regulating mitochondrial quality control  EMBO J   2014  33(4):282-95  PubMed ID: 24446486   DOI: 10.1002/embj.201385902
16009  Sharma M, Bhattacharyya S, Nain M, Kaur M, Sood V, Gupta V, Khasa R, Abdin MZ, Vrati S, Kalia M.  Japanese encephalitis virus replication is negatively regulated by autophagy and occurs on LC3-I- and EDEM1-containing membranes  Autophagy  2014  10(9):1637-51  PubMed ID: 25046112   DOI: 10.4161/auto.29455
9165  Hiroko Inoue, Katsunori Hase, Arata Segawa, Tsuyoshi Takita  H89 (N-[2-p-bromocinnamylamino-ethyl]-5-isoquinolinesulphonamide) Induces Autophagy Independently of Protein Kinase A Inhibition  Eur J Pharmacol   2013  714 (1-3), 170-7  PubMed ID: 23810683   DOI: 10.1016/j.ejphar.2013.06.018
9168  Shintaro Kobayashi, Tadaki Suzuki, Manabu Igarashi, Yasuko Orba, Noriko Ohtake, Keita Nagakawa, Kenichi Niikura, Takashi Kimura, Harumi Kasamatsu, Hirofumi Sawa  Cysteine Residues in the Major Capsid Protein, Vp1, of the JC Virus Are Important for Protein Stability and Oligomer Formation  PLoS One  2013  8 (10), e76668  PubMed ID: 24130786   DOI: 10.1371/journal.pone.0076668
9150  Yutaka Ueda, Fan-Yan Wei, Taku-ichiro Hide, Hiroyuki Michiue, Kentaro Takayama, Taku Kaitsuka, Hideo Nakamura, Keishi Makino, Jun-ichi Kuratsu, Shiroh Futaki, Kazuhito Tomizawa  Induction of Autophagic Cell Death of Glioma-Initiating Cells by Cell-Penetrating D-isomer Peptides Consisting of Pas and the p53 C-terminus  Biomaterials  2012  33 (35), 9061-9  PubMed ID: 23006589   DOI: 10.1016/j.biomaterials.2012.09.003
9153  Satoshi Tsuyuki, Sayuri Fukui, Akari Watanabe, Satoshi Akune, Mizuki Tanabe, Kenichi Yoshida  Delphinidin Induces Autolysosome as Well as Autophagosome Formation and Delphinidin-Induced Autophagy Exerts a Cell Protective Role  J Biochem Mol Toxicol  2012  26 (11), 445-53  PubMed ID: 23129091   DOI: 10.1002/jbt.21443
9154  Kai Er Eng, Marc D Panas, Deirdre Murphy, Gunilla B Karlsson Hedestam, Gerald M McInerney  Accumulation of Autophagosomes in Semliki Forest Virus-Infected Cells Is Dependent on Expression of the Viral Glycoproteins  J Virol  2012  86 (10), 5674-85  PubMed ID: 22438538   DOI: 10.1128/JVI.06581-11
7903  Tian Y, Bustos V, Flajolet M, Greengard P.  A small-molecule enhancer of autophagy decreases levels of Abeta and APP-CTF via Atg5-dependent autophagy pathway.  FASEB J.  2011  25:1934-42  PubMed ID: 21368103   DOI: 10.1096/fj.10-175158
7963  Tamboli IY, Hampel H, Tien NT, Tolksdorf K, Breiden B, Mathews PM, Saftig P, Sandhoff K, Walter J.  Sphingolipid storage affects autophagic metabolism of the amyloid precursor protein and promotes Abeta generation.  J. Neurosci.  2011  31:1837-49  PubMed ID: 21289194   DOI: 10.1523/JNEUROSCI.2954-10.2011
9148  Chengqun Huang, Allen M Andres, Eric P Ratliff, Genaro Hernandez, Pamela Lee, Roberta A Gottlieb  Preconditioning Involves Selective Mitophagy Mediated by Parkin and p62/SQSTM1  PLoS One  2011  6 (6), e20975  PubMed ID: 21687634   DOI: 10.1371/journal.pone.0020975
9152  Natura Myeku, Maria E Figueiredo-Pereira  Dynamics of the Degradation of Ubiquitinated Proteins by Proteasomes and Autophagy: Association With Sequestosome 1/p62  J Biol Chem  2011  286 (25), 22426-40  PubMed ID: 21536669   DOI: 10.1074/jbc.M110.149252
9163  Amy Colleran 1, Aideen Ryan, Angela O'Gorman, Coralie Mureau, Catherine Liptrot, Peter Dockery, Howard Fearnhead, Laurence J Egan  Autophagosomal IkappaB Alpha Degradation Plays a Role in the Long Term Control of Tumor Necrosis Factor-Alpha-Induced Nuclear factor-kappaB (NF-kappaB) Activity  J Biol Chem  2011  286 (26), 22886-93  PubMed ID: 21454695   DOI: 10.1074/jbc.M110.199950
8269  Eng KE, Panas MD, Karlsson Hedestam GB, McInerney GM.  A novel quantitative flow cytometry-based assay for autophagy.  Autophagy  2010  6:634-41  PubMed ID: 20458170   DOI: 10.4161/auto.6.5.12112

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