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Cell No. : Cell Name
RCB2709 : 2F8  update : 2021/05/20
CommentHybridoma producing monoclonal antibody against oxitocin receptor.
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Hashimoto, Masao
Originator Kimura, Tadashi
Year of deposit 2007
Animal mouse x mouse < Hybrid
Tissue hybridoma
Classification hybrid cells
Lifespan infinite
Morphology lymphocyte-like
Cellosaurus(Expasy) CVCL_J704
hybridoma Fusion_cell_1 name spleen cell
Fusion_cell_1 strain mouse, BALB/c
Fusion_cell_2 name P3X63Ag8U1
Fusion_cell_2 strain mouse, BALB/c
Fusion method PEG, HAT selection
Antibody name anti-OTR
Subclass IgM
Immunogen human oxytocin peptides
Antigen_name oxytocin receptor
Antigen_positive myometrium
deposit info
lot info
Medium Medium List
Culture type Suspension cells
Medium and additives DMEM/HamF12 + 10% FBS
Antibiotics Free
Passage method dilution
Culture information Passage ratio 1 : 6 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Antibody subclass IgM
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lot info
Reference information Reference 0
User's Publication 0


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