Cell No. : Cell Name RCB2709 : 2F8  update : 2025/07/25
Comment			Stopped distribution temporarily. Hybridoma producing monoclonal antibody against oxitocin receptor.
Comment from the depositor
Terms and conditions			There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form			Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)
			Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
	Basic information	Depositor	Hashimoto, Masao
		Originator	Kimura, Tadashi
		Year of deposit	2007
		Animal	_mouse x mouse < Hybrid
		Tissue	hybridoma
		Classification	hybrid cells
		Lifespan	infinite
		Morphology	lymphocyte-like
		Cellosaurus(Expasy)	CVCL_J704
	hybridoma	Fusion_cell_1 name	spleen cell
		Fusion_cell_1 strain	mouse, BALB/c
		Fusion_cell_2 name	P3X63Ag8U1
		Fusion_cell_2 strain	mouse, BALB/c
		Fusion method	PEG, HAT selection
		Antibody name	anti-OTR
		Subclass	IgM
		Immunogen	human oxytocin peptides
		Antigen_name	oxytocin receptor
		Antigen_positive	myometrium
			deposit info	lot info
		Medium	Medium List
		Culture type		Suspension cells
		Culture method		General Guidelines for Culturing Suspension Cells, 浮遊細胞の培養に関する一般的な注意(Japanese)
		Culture medium		DMEM/HamF12 + 10% FBS
		Antibiotics		Free
		Passage method		dilution
	Culture information	Passage ratio		1 : 6 split
		SC frequency		Subculture : 2 times/week
		Temperature		37 ℃
		CO2 concentration		5 %
		Freeze medium		Medium + 10% DMSO
		Freezing method		Slow freezing
		Mycoplasma/Acholeplasma		(-)
		Antibody subclass		IgM
Images			deposit info	lot info
Reference information		Reference	2
		User's Publication	0

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Reference

1894  Ito Y, Kobayashi T, Kimura T, Matsuura N, Wakasugi E, Takeda T, Shimano T, Kubota Y, Nobunaga T, Makino Y, Azuma C, Saji F, Monden M.  Investigation of the oxytocin receptor expression in human breast cancer tissue using newly established monoclonal antibodies  Endocrinology  1996  137(2):773-9  PubMed ID: 8593829   DOI: 10.1210/endo.137.2.8593829

2240  Takemura M, Kimura T, Nomura S, Makino Y, Inoue T, Kikuchi T, Kubota Y, Tokugawa Y, Nobunaga T, Kamiura S, et al.  Expression and localization of human oxytocin receptor mRNA and its protein in chorion and decidua during parturition  J Clin Invest  1994  93(6):2319-23  PubMed ID: 8200965   DOI: 10.1172/JCI117236

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