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Cell No. : Cell Name
RCB2700 : PK-8  update : 2024/09/11
CommentHuman cell line derived from liver metastasis of pancreas carcinoma. TKG0383 (Deposited from Tohoku Univ.).
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Fukuda, Hiroshi
Originator Kobari, Masao
Year of deposit 2007
Original cell TKG0383
Animal _human < Mammals
Genus Homo
Species sapiens
Tissue pancreas, liver meta
Disease name pancreas carcinoma
Metastatic ability Yes
Classification cancer
History Cell Resource Center for Biomedical Research, Tohoku University(TKG0383)
Lifespan infinite
Morphology epithelial-like
Cellosaurus(Expasy) CVCL_4718
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Culture medium RPMI1640 + 10% FBS
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma/Achoreplasma (-)
Isozyme LD, NP
STR(human) OK
Images
deposit info
lot info
Reference information Reference 1
User's Publication 34


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Reference
5253  Kobari M, Hisano H, Matsuno S, Sato T, Kan M, Tachibana T.  Establishment of six human pancreatic cancer cell lines and their sensitivities to anti-tumor drugs.  Tohoku J Exp Med  1986  150(3):231-48  PubMed ID: 3547771   DOI: 10.1620/tjem.150.231

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User's Publication
21765  Ferretti GDS, Quaas CE, Bertolini I, Zuccotti A, Saatci O, Kashatus JA, Sharmin S, Lu DY, Poli ANR, Quesnelle AF, Rodriguez-Blanco J, de Cubas AA, Hobbs GA, Liu Q, O'Bryan JP, Salvino JM, Kashatus DF, Sahin O, Barnoud T.  HSP70-mediated mitochondrial dynamics and autophagy represent a novel vulnerability in pancreatic cancer.  Cell Death Differ  2024    PubMed ID: 38802657   DOI: 10.1038/s41418-024-01310-9
18285  Yamakawa K, Koyanagi-Aoi M, Machinaga A, Kakiuchi N, Hirano T, Kodama Y, Aoi T.  Blockage of retinoic acid signaling via RARγ suppressed the proliferation of pancreatic cancer cells by arresting the cell cycle progression of the G1-S phase  Cancer Cell Int  2023  23(1):94  PubMed ID: 37198667   DOI: 10.1186/s12935-023-02928-4
18288  Kudo Y, Suzuki H, Tanaka T, Kaneko MK, Kato Y.  Development of a Novel Anti-CD44 Variant 5 Monoclonal Antibody C44Mab-3 for Multiple Applications against Pancreatic Carcinomas  Antibodies (Basel)  2023  12(2):31  PubMed ID: 37218897   DOI: 10.3390/antib12020031
19722  Masuda T, Fukuda A, Yamakawa G, Omatsu M, Namikawa M, Sono M, Fukunaga Y, Nagao M, Araki O, Yoshikawa T, Ogawa S, Masuo K, Goto N, Hiramatsu Y, Muta Y, Tsuda M, Maruno T, Nakanishi Y, Masui T, Hatano E, Matsuzaki T, Noda M, Seno H.  Pancreatic RECK inactivation promotes cancer formation, epithelial-mesenchymal transition, and metastasis  J Clin Invest  2023  133(18):e161847  PubMed ID: 37712427   DOI: 10.1172/JCI161847
19807  Shichi Y, Gomi F, Hasegawa Y, Nonaka K, Shinji S, Takahashi K, Ishiwata T.  Artificial intelligence-based analysis of time-lapse images of sphere formation and process of plate adhesion and spread of pancreatic cancer cells  Front Cell Dev Biol  2023  11:1290753  PubMed ID: 38046666   DOI: 10.3389/fcell.2023.1290753
21397  Sathiyaseelan P, Chittaranjan S, Kalloger SE, Chan J, Go NE, Jardon MA, Ho CJ, Hui T, Xu J, Chow C, Gao D, Johnson FD, Lockwood WW, Morin GB, Renouf DJ, Schaeffer DF, Gorski SM.  ATG4B and ATG4A loss result in two-stage cell cycle defects in pancreatic ductal adenocarcinoma cells.  J Cell Sci  2023    PubMed ID: 37701987   DOI: 10.1242/jcs.260644
21412  Kitamura F, Semba T, Yasuda-Yoshihara N, Yamada K, Nishimura A, Yamasaki J, Nagano O, Yasuda T, Yonemura A, Tong Y, Wang H, Akiyama T, Matsumura K, Uemura N, Itoyama R, Bu L, Fu L, Hu X, Wei F, Mima K, Imai K, Hayashi H, Yamashita YI, Miyamoto Y, Baba H, Ishimoto T.  Cancer-associated fibroblasts reuse cancer-derived lactate to maintain a fibrotic and immunosuppressive microenvironment in pancreatic cancer.  JCI Insight  2023    PubMed ID: 37733442   DOI: 10.1172/jci.insight.163022
17527  Sasaki N, Hirano K, Shichi Y, Gomi F, Yoshimura H, Matsushita A, Toyoda M, Ishiwata T.  Gp130-Mediated STAT3 Activation Contributes to the Aggressiveness of Pancreatic Cancer through H19 Long Non-Coding RNA Expression  Cancers (Basel)  2022  14(9):2055  PubMed ID: 35565185   DOI: 10.3390/cancers14092055
18221  Shichi Y, Gomi F, Sasaki N, Nonaka K, Arai T, Ishiwata T.  Epithelial and Mesenchymal Features of Pancreatic Ductal Adenocarcinoma Cell Lines in Two- and Three-Dimensional Cultures  J Pers Med  2022  12(5):746  PubMed ID: 35629168   DOI: 10.3390/jpm12050746
20575  Hassan G, Zahra MH, Seno A, Seno M.  The significance of ErbB2/3 in the conversion of induced pluripotent stem cells into cancer stem cells.  Sci Rep  2022  12(1):2711  PubMed ID: 35177646   DOI: 10.1038/s41598-022-04980-y
20717  Yamakawa K, Koyanagi-Aoi M, Uehara K, Masuda A, Yanagimoto H, Toyama H, Fukumoto T, Kodama Y, Aoi T.  Increased expression of SPRR1A is associated with a poor prognosis in pancreatic ductal adenocarcinoma.  PLoS One  2022  17(5)  PubMed ID: 35617311   DOI: 10.1371/journal.pone.0266620
20864  Shichi Y, Gomi F, Ueda Y, Nonaka K, Hasegawa F, Hasegawa Y, Hinata N, Yoshimura H, Yamamoto M, Takahashi K, Arai T, Ishiwata T.  Multiple cystic sphere formation from PK-8 cells in three-dimensional culture.  Biochem Biophys Rep  2022  32:101339  PubMed ID: 36105614   DOI: 10.1016/j.bbrep.2022.101339
20976  Liu Z, Hayashi H, Matsumura K, Ogata Y, Sato H, Shiraishi Y, Uemura N, Miyata T, Higashi T, Nakagawa S, Mima K, Imai K, Baba H.  Hyperglycaemia induces metabolic reprogramming into a glycolytic phenotype and promotes epithelial-mesenchymal transitions via YAP/TAZ-Hedgehog signalling axis in pancreatic cancer.  Br J Cancer  2022    PubMed ID: 36536047   DOI: 10.1038/s41416-022-02106-9
20074  Gomi F, Sasaki N, Shichi Y, Minami F, Shinji S, Toyoda M, Ishiwata T.  Polyvinyl alcohol increased growth, migration, invasion, and sphere size in the PK-8 pancreatic ductal adenocarcinoma cell line.  Heliyon  2021  7(2):e06182  PubMed ID: 33598581   DOI: 10.1016/j.heliyon.2021.e06182
20090  Minami F, Sasaki N, Shichi Y, Gomi F, Michishita M, Ohkusu-Tsukada K, Toyoda M, Takahashi K, Ishiwata T.  Morphofunctional analysis of human pancreatic cancer cell lines in 2- and 3-dimensional cultures.  Sci Rep  2021  11(1):6775  PubMed ID: 33762591   DOI: 10.1038/s41598-021-86028-1
20108  Tasaki Y, Suzuki M, Katsushima K, Shinjo K, Iijima K, Murofushi Y, Naiki-Ito A, Hayashi K, Qiu C, Takahashi A, Tanaka Y, Kawaguchi T, Sugawara M, Kataoka T, Naito M, Miyata K, Kataoka K, Noda T, Gao W, Kataoka H, Takahashi S, Kimura K, Kondo Y.  Cancer-Specific Targeting of Taurine-Upregulated Gene 1 Enhances the Effects of Chemotherapy in Pancreatic Cancer.  Cancer Res  2021    PubMed ID: 33648930   DOI: 10.1158/0008-5472.CAN-20-3021
20247  Munekage E, Serada S, Tsujii S, Yokota K, Kiuchi K, Tominaga K, Fujimoto M, Kanda M, Uemura S, Namikawa T, Nomura T, Murakami I, Hanazaki K, Naka T.  A glypican-1-targeted antibody-drug conjugate exhibits potent tumor growth inhibition in glypican-1-positive pancreatic cancer and esophageal squamous cell carcinoma.  Neoplasia  2021  23(9):939-950  PubMed ID: 34332450   DOI: 10.1016/j.neo.2021.07.006
16124  Nakao Y, Nakagawa S, Yamashita YI, Umezaki N, Okamoto Y, Ogata Y, Yasuda-Yoshihara N, Itoyama R, Yusa T, Yamashita K, Miyata T, Okabe H, Hayashi H, Imai K, Baba H.  High ARHGEF2 (GEF-H1) Expression is Associated with Poor Prognosis Via Cell Cycle Regulation in Patients with Pancreatic Cancer  Ann Surg Oncol  2021  28(8):4733-4743  PubMed ID: 33393038   DOI: 10.1245/s10434-020-09383-9
11820  Okazaki J, Tanahashi T, Sato Y, Miyoshi J, Nakagawa T, Kimura T, Miyamoto H, Fujino Y, Nakamura F, Takehara M, Ma B, Bando M, Kitamura S, Okamoto K, Muguruma N, Sogabe M, Takayama T.  MicroRNA-296-5p Promotes Cell Invasion and Drug Resistance by Targeting Bcl2-Related Ovarian Killer, Leading to a Poor Prognosis in Pancreatic Cancer.  Digestion  2020  101(6):794-806  PubMed ID: 31563901   DOI: 10.1159/000503225
13240  Onodera T, Momose I, Adachi H, Yamazaki Y, Sawa R, Ohba SI, Kawada M.  Human pancreatic cancer cells under nutrient deprivation are vulnerable to redox system inhibition.  J Biol Chem  2020    PubMed ID: 32978257   DOI: 10.1074/jbc.RA120.013893
13287  Sasaki N, Gomi F, Yoshimura H, Yamamoto M, Matsuda Y, Michishita M, Hatakeyama H, Kawano Y, Toyoda M, Korc M, Ishiwata T.  FGFR4 Inhibitor BLU9931 Attenuates Pancreatic Cancer Cell Proliferation and Invasion While Inducing Senescence: Evidence for Senolytic Therapy Potential in Pancreatic Cancer.  Cancers (Basel)  2020    PubMed ID: 33066597   DOI: 10.3390/cancers12102976
13542  Takehara M, Sato Y, Kimura T, Noda K, Miyamoto H, Fujino Y, Miyoshi J, Nakamura F, Wada H, Bando Y, Ikemoto T, Shimada M, Muguruma N, Takayama T.  Cancer-associated adipocytes promote pancreatic cancer progression through SAA1 expression  Cancer Sci  2020  111(8):2883-2894  PubMed ID: 32535957   DOI: 10.1111/cas.14527
14280  Hobbs GA, Baker NM, Miermont AM, Thurman RD, Pierobon M, Tran TH, Anderson AO, Waters AM, Diehl JN, Papke B, Hodge RG, Klomp JE, Goodwin CM, DeLiberty JM, Wang J, Ng RWS, Gautam P, Bryant KL, Esposito D, Campbell SL, Petricoin EF 3rd, Simanshu DK, Aguirre AJ, Wolpin BM, Wennerberg K, Rudloff U, Cox AD, Der CJ.  Atypical KRAS G12R Mutant Is Impaired in PI3K Signaling and Macropinocytosis in Pancreatic Cancer  Cancer Discov  2020  10(1):104-123  PubMed ID: 31649109   DOI: 10.1158/2159-8290.CD-19-1006
11218  Arima K, Ohmuraya M, Miyake K, Koiwa M, Uchihara T, Izumi D, Gao F, Yonemura A, Bu L, Okabe H, Imai K, Hashimoto D, Baba Y, Chikamoto A, Yamashita YI, Furukawa T, Araki K, Baba H, Ishimoto T.  Inhibition of 15-PGDH causes Kras-driven tumor expansion through prostaglandin E2-ALDH1 signaling in the pancreas.  Oncogene  2019  38(8):1211-1224  PubMed ID: 30250298   DOI: 10.1038/s41388-018-0510-y
5030  Tsukamoto M, Imai K, Ishimoto T, Komohara Y, Yamashita YI, Nakagawa S, Umezaki N, Yamao T, Kitano Y, Miyata T, Arima K, Okabe H, Baba Y, Chikamoto A, Ishiko T, Hirota M, Baba H.  PD-L1 expression enhancement by infiltrating macrophage-derived tumor necrosis factor-α leads to poor pancreatic cancer prognosis.  Cancer Sci  2019  110(1):310-320  PubMed ID: 30426611   DOI: 10.1111/cas.13874
11862  Afify SM, Chen L, Yan T, Calle AS, Nair N, Murakami C, Zahra MH, Okada N, Iwasaki Y, Seno A, Seno M.  Method to Convert Stem Cells into Cancer Stem Cells.  Methods Protoc  2019    PubMed ID: 31426391   DOI: 10.3390/mps2030071
11960  Sasaki N, Hirabayashi K, Michishita M, Takahashi K, Hasegawa F, Gomi F, Itakura Y, Nakamura N, Toyoda M, Ishiwata T.  Ganglioside GM2, highly expressed in the MIA PaCa-2 pancreatic ductal adenocarcinoma cell line, is correlated with growth, invasion, and advanced stage.  Sci Rep  2019    PubMed ID: 31852956   DOI: 10.1038/s41598-019-55867-4
12367  Mitsui Y, Tomonobu N, Watanabe M, Kinoshita R, Sumardika IW, Youyi C, Murata H, Yamamoto KI, Sadahira T, Rodrigo AGH, Takamatsu H, Araki K, Yamauchi A, Yamamura M, Fujiwara H, Inoue Y, Futami J, Saito K, Iioka H, Kondo E, Nishibori M, Toyooka S, Yamamoto Y, Nasu Y, Sakaguchi M.  Upregulation of Mobility in Pancreatic Cancer Cells by Secreted S100A11 Through Activation of Surrounding Fibroblasts  Oncol Res  2019  27(8):945-956.  PubMed ID: 31046874   DOI: 10.3727/096504019X15555408784978
12554  Takamatsu H, Yamamoto KI, Tomonobu N, Murata H, Inoue Y, Yamauchi A, Sumardika IW, Chen Y, Kinoshita R, Yamamura M, Fujiwara H, Mitsui Y, Araki K, Futami J, Saito K, Iioka H, Ruma IMW, Putranto EW, Nishibori M, Kondo E, Yamamoto Y, Toyooka S, Sakaguchi M.  Extracellular S100A11 Plays a Critical Role in Spread of the Fibroblast Population in Pancreatic Cancers  Oncol Res  2019  27(6):713-727  PubMed ID: 30850029   DOI: 10.3727/096504018X15433161908259
14880  Momose I, Onodera T, Doi H, Adachi H, Iijima M, Yamazaki Y, Sawa R, Kubota Y, Igarashi M, Kawada M.  Leucinostatin Y: A Peptaibiotic Produced by the Entomoparasitic Fungus Purpureocillium lilacinum 40-H-28  J Nat Prod  2019  82(5):1120-1127  PubMed ID: 31017786   DOI: 10.1021/acs.jnatprod.8b00839
10750  Arima K, Komohara Y, Bu L, Tsukamoto M, Itoyama R, Miyake K, Uchihara T, Ogata Y, Nakagawa S, Okabe H, Imai K, Hashimoto D, Chikamoto A, Yamashita YI, Baba H, Ishimoto T.  Downregulation of 15-hydroxyprostaglandin dehydrogenase by interleukin-1β from activated macrophages leads to poor prognosis in pancreatic cancer.  Cancer Sci.  2018  109:462-470  PubMed ID: 29224225   DOI: 10.1111/cas.13467
7044  Calle AS, Nair N, Oo AK, Prieto-Vila M, Koga M, Khayrani AC, Hussein M, Hurley L, Vaidyanath A, Seno A, Iwasaki Y, Calle M, Kasai T, Seno M.  A new PDAC mouse model originated from iPSCs-converted pancreatic cancer stem cells (CSCcm).  Am J Cancer Res  2016  6:2799-2815  PubMed ID: 28042501  
9938  Komura D, Isagawa T, Kishi K, Suzuki R, Sato R, Tanaka M, Katoh H, Yamamoto S, Tatsuno K, Fukayama M, Aburatani H, Ishikawa S.  CASTIN: a system for comprehensive analysis of cancer-stromal interactome.  BMC Genomics  2016  17:899  PubMed ID: 27829362   DOI: 10.1186/s12864-016-3207-z
12941  Saho S, Satoh H, Kondo E, Inoue Y, Yamauchi A, Murata H, Kinoshita R, Yamamoto KI, Futami J, Putranto EW, Ruma IM, Sumardika IW, Youyi C, Suzawa K, Yamamoto H, Soh J, Tomida S, Sakaguchi Y, Saito K, Iioka H, Huh NH, Toyooka S, Sakaguchi M.  Active Secretion of Dimerized S100A11 Induced by the Peroxisome in Mesothelioma Cells  Cancer Microenviron  2016  9(2-3):93-105  PubMed ID: 27334300   DOI: 10.1007/s12307-016-0185-2



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