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Cell No. : Cell Name
RCB2700 : PK-8  update : 2021/02/26
CommentHuman cell line derived from liver metastasis of pancreas carcinoma. TKG0383 (Deposited from Tohoku Univ.).
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor Fukuda, Hiroshi
Originator Kobari, Masao
Year of deposit 2007
Original cell TKG0383
Animal _human < Mammals
Genus Homo
Species sapiens
Tissue pancreas, liver meta
Disease name pancreas carcinoma
Metastastatic ability Yes
Classification cancer
History Cell Resource Center for Biomedical Research, Tohoku University(TKG0383)
Lifespan infinite
Morphology epithelial-like
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives RPMI1640 + 10% FBS
Antibiotics Free
Passage method 0.25% trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
STR(human) OK
Isozyme LD, NP
deposit info
lot info
Reference information Reference 1
User's Publication 17

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5253  Kobari M, Hisano H, Matsuno S, Sato T, Kan M, Tachibana T.  Establishment of six human pancreatic cancer cell lines and their sensitivities to anti-tumor drugs.  Tohoku J Exp Med  1986  150(3):231-48  PubMed ID: 3547771

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User's Publication
20074  Gomi F, Sasaki N, Shichi Y, Minami F, Shinji S, Toyoda M, Ishiwata T.  Polyvinyl alcohol increased growth, migration, invasion, and sphere size in the PK-8 pancreatic ductal adenocarcinoma cell line.  Heliyon  2021  7(2):e06182  PubMed ID: 33598581
20090  Minami F, Sasaki N, Shichi Y, Gomi F, Michishita M, Ohkusu-Tsukada K, Toyoda M, Takahashi K, Ishiwata T.  Morphofunctional analysis of human pancreatic cancer cell lines in 2- and 3-dimensional cultures.  Sci Rep  2021  11(1):6775  PubMed ID: 33762591
20108  Tasaki Y, Suzuki M, Katsushima K, Shinjo K, Iijima K, Murofushi Y, Naiki-Ito A, Hayashi K, Qiu C, Takahashi A, Tanaka Y, Kawaguchi T, Sugawara M, Kataoka T, Naito M, Miyata K, Kataoka K, Noda T, Gao W, Kataoka H, Takahashi S, Kimura K, Kondo Y.  Cancer-Specific Targeting of Taurine-Upregulated Gene 1 Enhances the Effects of Chemotherapy in Pancreatic Cancer.  Cancer Res  2021    PubMed ID: 33648930
13240  Onodera T, Momose I, Adachi H, Yamazaki Y, Sawa R, Ohba SI, Kawada M.  Human pancreatic cancer cells under nutrient deprivation are vulnerable to redox system inhibition.  J Biol Chem  2020    PubMed ID: 32978257
13287  Sasaki N, Gomi F, Yoshimura H, Yamamoto M, Matsuda Y, Michishita M, Hatakeyama H, Kawano Y, Toyoda M, Korc M, Ishiwata T.  FGFR4 Inhibitor BLU9931 Attenuates Pancreatic Cancer Cell Proliferation and Invasion While Inducing Senescence: Evidence for Senolytic Therapy Potential in Pancreatic Cancer.  Cancers (Basel)  2020    PubMed ID: 33066597
11218  Arima K, Ohmuraya M, Miyake K, Koiwa M, Uchihara T, Izumi D, Gao F, Yonemura A, Bu L, Okabe H, Imai K, Hashimoto D, Baba Y, Chikamoto A, Yamashita YI, Furukawa T, Araki K, Baba H, Ishimoto T.  Inhibition of 15-PGDH causes Kras-driven tumor expansion through prostaglandin E2-ALDH1 signaling in the pancreas.  Oncogene  2019  38(8):1211-1224  PubMed ID: 30250298
5030  Tsukamoto M, Imai K, Ishimoto T, Komohara Y, Yamashita YI, Nakagawa S, Umezaki N, Yamao T, Kitano Y, Miyata T, Arima K, Okabe H, Baba Y, Chikamoto A, Ishiko T, Hirota M, Baba H.  PD-L1 expression enhancement by infiltrating macrophage-derived tumor necrosis factor-α leads to poor pancreatic cancer prognosis.  Cancer Sci  2019  110(1):310-320  PubMed ID: 30426611
11820  Okazaki J, Tanahashi T, Sato Y, Miyoshi J, Nakagawa T, Kimura T, Miyamoto H, Fujino Y, Nakamura F, Takehara M, Ma B, Bando M, Kitamura S, Okamoto K, Muguruma N, Sogabe M, Takayama T.  MicroRNA-296-5p Promotes Cell Invasion and Drug Resistance by Targeting Bcl2-Related Ovarian Killer, Leading to a Poor Prognosis in Pancreatic Cancer.  Digestion  2019    PubMed ID: 31563901
11862  Afify SM, Chen L, Yan T, Calle AS, Nair N, Murakami C, Zahra MH, Okada N, Iwasaki Y, Seno A, Seno M.  Method to Convert Stem Cells into Cancer Stem Cells.  Methods Protoc  2019    PubMed ID: 31426391
11960  Sasaki N, Hirabayashi K, Michishita M, Takahashi K, Hasegawa F, Gomi F, Itakura Y, Nakamura N, Toyoda M, Ishiwata T.  Ganglioside GM2, highly expressed in the MIA PaCa-2 pancreatic ductal adenocarcinoma cell line, is correlated with growth, invasion, and advanced stage.  Sci Rep  2019    PubMed ID: 31852956
12367  Mitsui Y, Tomonobu N, Watanabe M, Kinoshita R, Sumardika IW, Youyi C, Murata H, Yamamoto KI, Sadahira T, Rodrigo AGH, Takamatsu H, Araki K, Yamauchi A, Yamamura M, Fujiwara H, Inoue Y, Futami J, Saito K, Iioka H, Kondo E, Nishibori M, Toyooka S, Yamamoto Y, Nasu Y, Sakaguchi M.  Upregulation of Mobility in Pancreatic Cancer Cells by Secreted S100A11 Through Activation of Surrounding Fibroblasts  Oncol Res  2019  27(8):945-956.  PubMed ID: 31046874
12554  Takamatsu H, Yamamoto KI, Tomonobu N, Murata H, Inoue Y, Yamauchi A, Sumardika IW, Chen Y, Kinoshita R, Yamamura M, Fujiwara H, Mitsui Y, Araki K, Futami J, Saito K, Iioka H, Ruma IMW, Putranto EW, Nishibori M, Kondo E, Yamamoto Y, Toyooka S, Sakaguchi M.  Extracellular S100A11 Plays a Critical Role in Spread of the Fibroblast Population in Pancreatic Cancers  Oncol Res  2019  27(6):713-727  PubMed ID: 30850029
10750  Arima K, Komohara Y, Bu L, Tsukamoto M, Itoyama R, Miyake K, Uchihara T, Ogata Y, Nakagawa S, Okabe H, Imai K, Hashimoto D, Chikamoto A, Yamashita YI, Baba H, Ishimoto T.  Downregulation of 15-hydroxyprostaglandin dehydrogenase by interleukin-1β from activated macrophages leads to poor prognosis in pancreatic cancer.  Cancer Sci.  2018  109:462-470  PubMed ID: 29224225
7044  Calle AS, Nair N, Oo AK, Prieto-Vila M, Koga M, Khayrani AC, Hussein M, Hurley L, Vaidyanath A, Seno A, Iwasaki Y, Calle M, Kasai T, Seno M.  A new PDAC mouse model originated from iPSCs-converted pancreatic cancer stem cells (CSCcm).  Am J Cancer Res  2016  6:2799-2815  PubMed ID: 28042501
9938  Komura D, Isagawa T, Kishi K, Suzuki R, Sato R, Tanaka M, Katoh H, Yamamoto S, Tatsuno K, Fukayama M, Aburatani H, Ishikawa S.  CASTIN: a system for comprehensive analysis of cancer-stromal interactome.  BMC Genomics  2016  17:899  PubMed ID: 27829362
6031  Saho S et al  Active Secretion of Dimerized S100A11 Induced by the Peroxisome in Mesothelioma Cells.  Cancer Microenviron  2016  9(2-3):93-105 
12941  Saho S, Satoh H, Kondo E, Inoue Y, Yamauchi A, Murata H, Kinoshita R, Yamamoto KI, Futami J, Putranto EW, Ruma IM, Sumardika IW, Youyi C, Suzawa K, Yamamoto H, Soh J, Tomida S, Sakaguchi Y, Saito K, Iioka H, Huh NH, Toyooka S, Sakaguchi M.  Active Secretion of Dimerized S100A11 Induced by the Peroxisome in Mesothelioma Cells  Cancer Microenviron  2016  9(2-3):93-105  PubMed ID: 27334300

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