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Cell No. : Cell Name
RCB2696 : RD-C6  update : 2023/10/16
CommentMouse osteoblast-like cell line derived from Runx2 deficient mouse.
Comment from the depositor
Terms and conditionsIn publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature ref. (J Cell Physiol 2007;211:728-735) designated by the DEPOSITOR is required.
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor Yamaguchi, Akira
Originator Yamaguchi, Akira
Year of deposit 2007
Animal _mouse < Mammals
Strain name C57BL/6
Age at sampling 18.5 days embryo
Tissue calvaria
Disease name Runx2-deficient mouse
Classification other
Lifespan infinite
Morphology fibroblast-like
Cellosaurus(Expasy) CVCL_8749
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Culture medium MEM ALPHA + 10% FBS
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
SSLP(mouse) OK
Isozyme LD, NP
deposit info
lot info
Reference information Reference 1
User's Publication 0

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3620  Liu T, Gao Y, Sakamoto K, Minamizato T, Furukawa K, Tsukazaki T, Shibata Y, Bessho K, Komori T, Yamaguchi A.  BMP-2 promotes differentiation of osteoblasts and chondroblasts in Runx2-deficient cell lines.  J Cell Physiol  2007  211(3):728-35  PubMed ID: 17226753   DOI: 10.1002/jcp.20988

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