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Cell No. : Cell Name
RCB2696 : RD-C6 update : 2023/10/16 |
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| Comment | Mouse osteoblast-like cell line derived from Runx2 deficient mouse. | |||
| Comment from the depositor | ||||
| Terms and conditions | In publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature ref. (J Cell Physiol 2007;211:728-735) designated by the DEPOSITOR is required. | |||
| Remarks | ||||
| Order Form | Order Form(C-0005.pdf) MTA(C-0007.pdf) MTA(C-0007p.pdf) | |||
| Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp). | ||||
| Basic information | Depositor | Yamaguchi, Akira | ||
| Originator | Yamaguchi, Akira | |||
| Year of deposit | 2007 | |||
| Animal | _mouse < Mammals | |||
| Strain name | C57BL/6 | |||
| Age at sampling | 18.5 days embryo | |||
| Tissue | calvaria | |||
| Disease name | Runx2-deficient mouse | |||
| Classification | other | Lifespan | infinite | |
| Morphology | fibroblast-like | |||
| Cellosaurus(Expasy) | CVCL_8749 | |||
| Medium | Medium List | Culture type | Adherent cells | |
| Culture medium | MEM ALPHA + 10% FBS | |||
| Antibiotics | Free | Passage method | 0.25% Trypsin | Culture information | Passage ratio | 1 : 8 split |
| SC frequency | Subculture : 2 times/week | |||
| Temperature | 37 ℃ | |||
| CO2 concentration | 5 % | |||
| Freeze medium | Medium + 10% DMSO | |||
| Freezing method | Slow freezing | |||
| Mycoplasma/Acholeplasma | (-) | |||
| SSLP(mouse) | OK | |||
| Isozyme | LD, NP | |||
| Images | ||||
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| Reference information | Reference | 1 | ||
| User's Publication | 0 | |||
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