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Cell No. : Cell Name
RCB2694 : MEDMC-BRC6  update : 2018/07/06
CommentMouse cell line derived from mouse ES cell line, BRC6 (AES0010). Mast cell-like cells. SCF (Stem cell factor) and IL-3 (Interleukin-3) dependent. C57BL/6 strain.
Comment from the depositor
Terms and conditionsIn publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature ref. (PLoS ONE 2008;3:e1544) designated by the DEPOSITOR is required.
Remarks
Order Form Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use.Please use an appropriate MTA(to see).In case of Restriction "a" or "f", please contact RIKEN BRC(cellbank.brc@riken.jp) regarding any kind of for-profit use.
Basic information Depositor Hiroyama, Takashi
Originator Hiroyama, Takashi
Year of deposit 2007
Animal mouse < Mammals
Strain name C57BL/6
Gender Female
Age at sampling embryo
Tissue embryonic stem cells : BRC6(AES010)
Classification other
Lifespan infinite
Morphology lymphocyte-like
deposit info
lot info
Medium Medium List
Culture type Suspension cells
Medium and additives IMDM (without L-Glutamine) + 2mM L-Glutamine + 15% FBS + ITS (SIGMA #I3146) + 0.45mM α-monothioglycerol + 50μg/ml Ascorbic acid + 30ng/ml mouse SCF + 3ng/ml IL-3
Antibiotics Free
Passage method dilution
Culture information Passage ratio 1 : 5 split
SC frequency Subculture : 3 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
SSLP(mouse) OK
Isozyme LD, NP
Images
deposit info
lot info
Reference information Reference 1
User's Publication 2


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Reference
8770  Hiroyama T, Miharada K, Sudo K, Danjo I, Aoki N, Nakamura Y.  Establishment of mouse embryonic stem cell-derived erythroid progenitor cell lines able to produce functional red blood cells.  PLoS ONE  2008  3:e1544  PubMed ID: 18253492

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User's Publication
11799  Ohmori S, Ishijima Y, Numata S, Takahashi M, Sekita M, Sato T, Chugun K, Yamamoto M, Ohneda K.  GATA2 and PU.1 Collaborate To Activate the Expression of the Mouse Ms4a2 Gene, Encoding FcεRIβ, through Distinct Mechanisms.  Mol. Cell. Biol.  2019    PubMed ID: 31501274
6057  Kanemaru K et al  Tie2 Signaling Enhances Mast Cell Progenitor Adhesion to Vascular Cell Adhesion Molecule-1 (VCAM-1) through α4β1 Integrin.  PLoS One  2015  10(12):e0144436  PubMed ID: 26659448



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