Cell No. : Cell Name
RCB2694 : MEDMC-BRC6
update : 2018/07/06
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| Comment | Mouse cell line derived from mouse ES cell line, BRC6 (AES0010). Mast cell-like cells. SCF (Stem cell factor) and IL-3 (Interleukin-3) dependent. C57BL/6 strain. |
| Comment from the depositor | |
| Terms and conditions | In publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature ref. (PLoS ONE 2008;3:e1544) designated by the DEPOSITOR is required. |
| Remarks | |
| Order Form |
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use.Please use an appropriate MTA(to see).In case of Restriction "a" or "f", please contact RIKEN BRC(cellbank.brc@riken.jp) regarding any kind of for-profit use.
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Basic information
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Depositor |
Hiroyama, Takashi
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| Originator |
Hiroyama, Takashi
|
| Year of deposit |
2007
|
| Animal |
mouse
< Mammals
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| Strain name |
C57BL/6
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| Gender |
Female
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| Age at sampling |
embryo
|
| Tissue |
embryonic stem cells : BRC6(AES010)
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| Classification |
other
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|
Lifespan |
infinite
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| Morphology |
lymphocyte-like
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deposit info |
lot info |
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Medium |
Medium List |
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Culture type |
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Suspension cells
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| Medium and additives |
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IMDM (without L-Glutamine) + 2mM L-Glutamine + 15% FBS + ITS (SIGMA #I3146) + 0.45mM α-monothioglycerol + 50μg/ml Ascorbic acid + 30ng/ml mouse SCF + 3ng/ml IL-3
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| Antibiotics |
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Free
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Passage method |
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dilution
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Culture information
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Passage ratio |
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1 : 5 split
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| SC frequency |
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Subculture : 3 times/week
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| Temperature |
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37
℃
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| CO2 concentration |
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5
%
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| Freeze medium |
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Medium + 10% DMSO
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| Freezing method |
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Slow freezing
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| Mycoplasma |
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(-)
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| SSLP(mouse) |
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OK
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| Isozyme |
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LD, NP
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| Images |
deposit info | lot info |
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| Reference information |
Reference |
1
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| User's Publication |
2
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