Cell No. : Cell Name
RCB2694 : MEDMC-BRC6
update : 2024/01/17
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| Comment | Mouse cell line derived from mouse ES cell line, BRC6 (AES0010). Mast cell-like cells. SCF (Stem cell factor) and IL-3 (Interleukin-3) dependent. C57BL/6 strain. |
| Comment from the depositor | |
| Terms and conditions | In publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature ref. (PLoS ONE 2008;3:e1544) designated by the DEPOSITOR is required. |
| Remarks | |
| Order Form |
Order Form(C-0005.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
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Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
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Depositor |
Hiroyama, Takashi
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| Originator |
Hiroyama, Takashi
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| Year of deposit |
2007
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| Animal |
_mouse
< Mammals
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| Strain name |
C57BL/6
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| Gender |
Female
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| Age at sampling |
embryo
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| Tissue |
embryonic stem cells : BRC6(AES010)
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| Classification |
other
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Lifespan |
infinite
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| Morphology |
lymphocyte-like
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| Cellosaurus(Expasy) |
CVCL_E140
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deposit info |
lot info |
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Medium |
Medium List |
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Culture type |
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Suspension cells
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| Culture method |
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General Guidelines for Culturing Suspension Cells, 浮遊細胞の培養に関する一般的な注意(Japanese)
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| Culture medium |
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IMDM (without L-Glutamine) + 2mM L-Glutamine + 15% FBS + ITS (SIGMA #I3146) + 0.45mM α-monothioglycerol + 50μg/ml Ascorbic acid + 30ng/ml mouse SCF + 3ng/ml IL-3
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| Antibiotics |
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Free
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Passage method |
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dilution
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Culture information
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Passage ratio |
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1 : 5 split
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| SC frequency |
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Subculture : 3 times/week
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| Temperature |
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37
℃
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| CO2 concentration |
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5
%
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| Freeze medium |
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Medium + 10% DMSO
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| Freezing method |
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Slow freezing
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| Mycoplasma/Acholeplasma |
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(-)
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| SSLP(mouse) |
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OK
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| Isozyme |
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LD, NP
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| Images |
deposit info | lot info |
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| Reference information |
Reference |
2
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| User's Publication |
3
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| Reference |
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14679
Shibagaki S, Tahara-Hanaoka S, Hiroyama T, Nakamura Y, Shibuya A.
Long-term survival of the mouse ES cell-derived mast cell, MEDMC-BRC6, in mast cell-deficient KitW-sh/W-sh mice
Int Immunol
2017
29(5):235-242
PubMed ID: 28431112
DOI: 10.1093/intimm/dxx022
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8770
Hiroyama T, Miharada K, Sudo K, Danjo I, Aoki N, Nakamura Y.
Establishment of mouse embryonic stem cell-derived erythroid progenitor cell lines able to produce functional red blood cells.
PLoS ONE
2008
3:e1544
PubMed ID: 18253492
DOI: 10.1371/journal.pone.0001544
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| User's Publication |
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20860
Ohmori S, Takai J, Uemura S, Otsuki A, Mori T, Ohneda K, Moriguchi T.
The Il6 -39 kb enhancer containing clustered GATA2- and PU.1-binding sites is essential for Il6 expression in murine mast cells.
iScience
2022
25(9):104942
PubMed ID: 36072552
DOI: 10.1016/j.isci.2022.104942
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11799
Ohmori S, Ishijima Y, Numata S, Takahashi M, Sekita M, Sato T, Chugun K, Yamamoto M, Ohneda K.
GATA2 and PU.1 Collaborate To Activate the Expression of the Mouse Ms4a2 Gene, Encoding FcεRIβ, through Distinct Mechanisms.
Mol. Cell. Biol.
2019
PubMed ID: 31501274
DOI: 10.1128/MCB.00314-19
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6057
Kanemaru K et al
Tie2 Signaling Enhances Mast Cell Progenitor Adhesion to Vascular Cell Adhesion Molecule-1 (VCAM-1) through α4β1 Integrin.
PLoS One
2015
10(12):e0144436
PubMed ID: 26659448
DOI: 10.1371/journal.pone.0144436
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