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Cell No. : Cell Name
RCB2694 : MEDMC-BRC6  update : 2024/01/17
CommentMouse cell line derived from mouse ES cell line, BRC6 (AES0010). Mast cell-like cells. SCF (Stem cell factor) and IL-3 (Interleukin-3) dependent. C57BL/6 strain.
Comment from the depositor
Terms and conditionsIn publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature ref. (PLoS ONE 2008;3:e1544) designated by the DEPOSITOR is required.
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor Hiroyama, Takashi
Originator Hiroyama, Takashi
Year of deposit 2007
Animal _mouse < Mammals
Strain name C57BL/6
Gender Female
Age at sampling embryo
Tissue embryonic stem cells : BRC6(AES010)
Classification other
Lifespan infinite
Morphology lymphocyte-like
Cellosaurus(Expasy) CVCL_E140
deposit info
lot info
Medium Medium List
Culture type Suspension cells
Culture medium IMDM (without L-Glutamine) + 2mM L-Glutamine + 15% FBS + ITS (SIGMA #I3146) + 0.45mM α-monothioglycerol + 50μg/ml Ascorbic acid + 30ng/ml mouse SCF + 3ng/ml IL-3
Culture method
Antibiotics Free
Passage method dilution
Culture information Passage ratio 1 : 5 split
SC frequency Subculture : 3 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
SSLP(mouse) OK
Isozyme LD, NP
deposit info
lot info
Reference information Reference 2
User's Publication 3

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14679  Shibagaki S, Tahara-Hanaoka S, Hiroyama T, Nakamura Y, Shibuya A.  Long-term survival of the mouse ES cell-derived mast cell, MEDMC-BRC6, in mast cell-deficient KitW-sh/W-sh mice  Int Immunol  2017  29(5):235-242  PubMed ID: 28431112   DOI: 10.1093/intimm/dxx022
8770  Hiroyama T, Miharada K, Sudo K, Danjo I, Aoki N, Nakamura Y.  Establishment of mouse embryonic stem cell-derived erythroid progenitor cell lines able to produce functional red blood cells.  PLoS ONE  2008  3:e1544  PubMed ID: 18253492   DOI: 10.1371/journal.pone.0001544

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User's Publication
20860  Ohmori S, Takai J, Uemura S, Otsuki A, Mori T, Ohneda K, Moriguchi T.  The Il6 -39 kb enhancer containing clustered GATA2- and PU.1-binding sites is essential for Il6 expression in murine mast cells.  iScience  2022  25(9):104942  PubMed ID: 36072552   DOI: 10.1016/j.isci.2022.104942
11799  Ohmori S, Ishijima Y, Numata S, Takahashi M, Sekita M, Sato T, Chugun K, Yamamoto M, Ohneda K.  GATA2 and PU.1 Collaborate To Activate the Expression of the Mouse Ms4a2 Gene, Encoding FcεRIβ, through Distinct Mechanisms.  Mol. Cell. Biol.  2019    PubMed ID: 31501274   DOI: 10.1128/MCB.00314-19
6057  Kanemaru K et al  Tie2 Signaling Enhances Mast Cell Progenitor Adhesion to Vascular Cell Adhesion Molecule-1 (VCAM-1) through α4β1 Integrin.  PLoS One  2015  10(12):e0144436  PubMed ID: 26659448   DOI: 10.1371/journal.pone.0144436

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