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Cell No. : Cell Name
RCB2692 : MEDEP-BRC4-1  update : 2023/03/15
CommentMouse erythroid cell line able to differentiate into enucleated red blood cells. Derived from mouse ES cell line, BRC4 (AES0008). SCF(Stem cell factor) and Dex(dexamethasone) dependent.
Comment from the depositor
Terms and conditionsIn publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature ref. (PLoS ONE 2008;3:e1544) designated by the DEPOSITOR is required.
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Basic information Depositor Hiroyama, Takashi
Originator Hiroyama, Takashi
Year of deposit 2007
Animal _mouse < Mammals
Strain name C57BL/6
Gender Unknown
Age at sampling embryo
Tissue embryonic stem cells : BRC4(RCB2008)
Classification other
Lifespan infinite
Morphology lymphocyte-like
Cellosaurus(Expasy) CVCL_E139
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lot info
Medium Medium List
Culture type Suspension cells
Culture medium IMDM (without L-Glutamine) + 2mM L-Glutamine + 15% FBS + ITS (SIGMA #I3146) + 0.45mM α-monothioglycerol + 10 -6M Dexamethasone + 50μg/ml Ascorbic acid + 30ng/ml mouse SCF
Antibiotics Free
Passage method dilution
Culture information Passage ratio 1 : 5 split
SC frequency Subculture : 3 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
SSLP(mouse) OK
Isozyme LD, NP
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lot info
Reference information Reference 1
User's Publication 0

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8770  Hiroyama T, Miharada K, Sudo K, Danjo I, Aoki N, Nakamura Y.  Establishment of mouse embryonic stem cell-derived erythroid progenitor cell lines able to produce functional red blood cells.  PLoS ONE  2008  3:e1544  PubMed ID: 18253492   DOI: 10.1371/journal.pone.0001544

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