Back Back
Cell No. : Cell Name
RCB2660 : MEDEP-E14  update : 2024/01/17
CommentMouse erythroid cell line able to differentiate into enucleated red blood cells. Derived from mouse ES cell line, E14TG2a. Epo(Erythropoietin) dependent.
Comment from the depositor
Terms and conditionsIn publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature ref. (PLoS ONE 2008;3:e1544) designated by the DEPOSITOR is required.
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor Hiroyama, Takashi
Originator Hiroyama, Takashi
Year of deposit 2007
Animal _mouse < Mammals
Strain name 129/Ola
Gender Male
Age at sampling embryo
Tissue embryonic stem cells : E14TG2a
Classification other
Lifespan infinite
Morphology lymphocyte-like
Cellosaurus(Expasy) CVCL_E138
deposit info
lot info
Medium Medium List
Culture type Suspension cells
Culture method 浮遊細胞の培養に関する一般的な注意(Japanese)
Culture medium IMDM (without L-Glutamine) + 2mM L-Glutamine + 15% FBS + ITS (SIGMA #I3146) + 0.45mM α-monothioglycerol + 50μg/ml Ascorbic acid + 3U/ml human EPO
Antibiotics Free
Passage method dilution
Culture information Passage ratio 1 : 10 split
SC frequency Subculture : 3 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
SSLP(mouse) OK
Isozyme LD, NP
deposit info
lot info
Reference information Reference 1
User's Publication 2

To topTop
8770  Hiroyama T, Miharada K, Sudo K, Danjo I, Aoki N, Nakamura Y.  Establishment of mouse embryonic stem cell-derived erythroid progenitor cell lines able to produce functional red blood cells.  PLoS ONE  2008  3:e1544  PubMed ID: 18253492   DOI: 10.1371/journal.pone.0001544

To topTop
User's Publication
20120  Goto H, Miyamoto M, Kihara A.  Direct uptake of sphingosine-1-phosphate (S1P) independent of phospholipid phosphatases.  J Biol Chem  2021    PubMed ID: 33785361   DOI: 10.1016/j.jbc.2021.100605
10792  Kobayashi N, Kawasaki-Nishi S, Otsuka M, Hisano Y, Yamaguchi A, Nishi T.  MFSD2B is a sphingosine 1-phosphate transporter in erythroid cells.  Sci Rep  2018  8:4969  PubMed ID: 29563527   DOI: 10.1038/s41598-018-23300-x

Back Back Return Top Page