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Cell No. : Cell Name
RCB2657 : Colon-26  update : 2021/02/26
CommentMouse cell line derived from colon cancer. BALB/c strain. TKG0518 (Deposited from Tohoku Univ.).
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor Fukuda, Hiroshi
Year of deposit 2007
Original cell TKG0518
Animal _mouse < Mammals
Strain name BALB/c
Tissue rectum
Disease name Rectum carcinoma
Classification cancer
History Cell Resource Center for Biomedical Research, Tohoku University(TKG0518)
Memo_1 A culture submitted to the CRCBR, Tohoku University was found to be contaminated with mycoplasma. Mycoplasma was eliminated with BM Cycline and MC210 at the CRCBR.
Lifespan infinite
Morphology fibroblast-like
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives RPMI1640 + 10% FBS
Antibiotics Free
Passage method 0.25% trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Animal PCR OK
SSLP(mouse) OK
Isozyme LD, NP
deposit info
lot info
Reference information Reference 1
User's Publication 67

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5251  Corbett TH, Griswold DP Jr, Roberts BJ, Peckham JC, Schabel FM Jr.  Tumor induction relationships in development of transplantable cancers of the colon in mice for chemotherapy assays, with a note on carcinogen structure.  Cancer Res  1975  35(9):2434-9  PubMed ID: 1149045

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User's Publication
11792  Mimura A, Inaoka Y, Asakawa S, Murase K.  A Novel Cellular Imaging Method Using Hemagglutinating Virus of Japan-Envelope (HVJ-E) Vector and Magnetic Particle Imaging.  J Nanosci Nanotechnol  2020    PubMed ID: 31492206
12035  Kitao A, Kawamoto S, Kurata K, Hayakawa I, Yamasaki T, Matsuoka H, Sumi Y, Kakeji Y, Kamesaki T, Minami H.  Band 3 ectopic expression in colorectal cancer induces an increase in erythrocyte membrane-bound IgG and may cause immune-related anemia.  Int. J. Hematol.  2020    PubMed ID: 31997080
12327  Mahib MR, Hosojima S, Kushiyama H, Kinoshita T, Shiroishi T, Suda T, Tsuchiya K.  Caspase-7 mediates caspase-1-induced apoptosis independently of Bid  Microbiol Immunol  2020  64(2):143-152  PubMed ID: 31687791
13004  Yamada T, Ashida Y, Tatebayashi D, Abe M, Himori K.  Cancer Cachexia Induces Preferential Skeletal Muscle Myosin Loss When Combined With Denervation.  Front Physiol  2020    PubMed ID: 32425814
13266  Nomura S, Takahashi M, Kato AH, Wada Y, Watanabe Y, Yamashita F, Mukai H.  Biosorption-based 64Cu-labeling of bacteria for pharmacokinetic positron-emission tomography.  Int J Pharm  2020    PubMed ID: 33027635
13300  Takayama T, Shimizu T, Lila ASA, Kanazawa Y, Ando H, Ishima Y, Ishida T.  Adjuvant Antitumor Immunity Contributes to the Overall Antitumor Effect of Pegylated Liposomal Doxorubicin (Doxil®) in C26 Tumor-Bearing Immunocompetent Mice.  Pharmaceutics  2020    PubMed ID: 33086690
13584  Uda RM, Yoshida N, Iwasaki T, Hayashi K.  pH-triggered solubility and cytotoxicity changes of malachite green derivatives incorporated in liposomes for killing cancer cells  J Mater Chem B  2020  8(36):8242-8248  PubMed ID: 32794526
13603  Imanishi M, Yamakawa Y, Fukushima K, Ikuto R, Maegawa A, Izawa-Ishizawa Y, Horinouchi Y, Kondo M, Kishuku M, Goda M, Zamami Y, Takechi K, Chuma M, Ikeda Y, Tsuchiya K, Fujino H, Tsuneyama K, Ishizawa K.  Fibroblast-specific ERK5 deficiency changes tumor vasculature and exacerbates tumor progression in a mouse model  Naunyn Schmiedebergs Arch Pharmacol  2020  393(7):1239-1250.  PubMed ID: 32307577
13631  Mei T, Shashni B, Maeda H, Nagasaki Y.  Fibrinolytic tissue plasminogen activator installed redox-active nanoparticles (t-PA@iRNP) for cancer therapy  Biomaterials  2020  259:120290  PubMed ID: 32829147
11341  Fukuda T, Nanjo Y, Fujimoto M, Yoshida K, Natsui Y, Ishibashi F, Okazaki F, To H, Iwao M.  Lamellarin-inspired potent topoisomerase I inhibitors with the unprecedented benzo[g][1]benzopyrano[4,3-b]indol-6(13H)-one scaffold.  Bioorg. Med. Chem.  2019    PubMed ID: 30553626
4573  Hatai M, Horiyama S, Yoshikawa N, Kinoshita E, Kagota S, Shinozuka K, Nakamura K.  trans-2-Pentenal, an Active Compound in Cigarette Smoke, Identified via Its Ability to Form Adducts with Glutathione.  Chem Pharm Bull (Tokyo)  2019  67(9):1000-1005  PubMed ID: 31474722
11506  Hayashi K, Watanabe M, Iwasaki T, Shudou M, Uda RM.  Endosomal escape by photo-activated fusion of liposomes containing a malachite green derivative: a novel class of photoresponsive liposomes for drug delivery vehicles.  Photochem. Photobiol. Sci.  2019    PubMed ID: 30964475
11509  Zhelev Z, Georgieva E, Lazarova D, Semkova S, Aoki I, Gulubova M, Higashi T, Bakalova R.  Redox Imaging to Distinguish Cells with Different Proliferative Indexes: Superoxide, Hydroperoxides, and Their Ratio as Potential Biomarkers.  Oxid Med Cell Longev  2019    PubMed ID: 31089411
11530  Uda RM, Nishimoto N, Matsui T, Takagi S.  Photoinduced binding of malachite green copolymer to parallel G-quadruplex DNA.  Soft Matter  2019    PubMed ID: 31073583
11539  Suzuka H, Mimura A, Inaoka Y, Murase K.  Magnetic Nanoparticles in Macrophages and Cancer Cells Exhibit Different Signal Behavior on Magnetic Particle Imaging.  J Nanosci Nanotechnol  2019    PubMed ID: 31039837
11546  Tsuchiya K, Nakajima S, Hosojima S, Thi Nguyen D, Hattori T, Manh Le T, Hori O, Mahib MR, Yamaguchi Y, Miura M, Kinoshita T, Kushiyama H, Sakurai M, Shiroishi T, Suda T.  Caspase-1 initiates apoptosis in the absence of gasdermin D.  Nat Commun  2019    PubMed ID: 31064994
11603  Aoki N, Matsui Y.  Comprehensive Analysis of Mouse Cancer/Testis Antigen Functions in Cancer Cells and Roles of TEKT5 in Cancer Cells and Testicular Germ Cells.  Mol. Cell. Biol.  2019    PubMed ID: 31208979
11847  Matsui S, Kataoka H, Tanaka JI, Kikuchi M, Fukamachi H, Morisaki H, Matsushima H, Mishima K, Hironaka S, Takaki T, Okahashi N, Maruoka Y, Kuwata H.  Dysregulation of intestinal microbiota elicited by food allergy induces IgA-mediated oral dysbiosis.  Infect. Immun.  2019    PubMed ID: 31611274
11859  Emam SE, Selim Abu Lila A, Elsadek NE, Ando H, Shimizu T, Okuhira K, Ishima Y, Mahdy MA, Ghazy FS, Ishid T.  Cancer cell-type tropism is one of crucial determinants for the efficient systemic delivery of cancer cell-derived exosomes to tumor tissues.  Eur J Pharm Biopharm  2019    PubMed ID: 31629787
9811  Eiji Kasumi, Norifumi Sato  A Ketogenic Diet Improves the Prognosis in a Mouse Model of Peritoneal Dissemination Without Tumor Regression  J Clin Biochem Nutr   2019  64(3):201-208  PubMed ID: 31138953
10421  Yoshimi Inaoka, Tamami Keii, Atsushi Mimura, Kenya Murase  A Novel Therapeutic Strategy Combining Use of Intracellular Magnetic Nanoparticles under an Alternating Magnetic Field and Bleomycin  Open Journal of Applied Sciences  2019  9, Article ID:91110,17 
10589  Seiji Komeda, Masako Uemura, Hiroki Yoneyama, Shinya Harusawa and Keiichi Hiramoto  In Vitro Cytotoxicity and In Vivo Antitumor Efficacy of Tetrazolato-Bridged Dinuclear Platinum(II) Complexes with a Bulky Substituent at Tetrazole C5  Inorganics  2019  7(1):5 
12407  Peng JQ, Fumoto S, Suga T, Miyamoto H, Kuroda N, Kawakami S, Nishida K.  Targeted co-delivery of protein and drug to a tumor in vivo by sophisticated RGD-modified lipid-calcium carbonate nanoparticles  J Control Release  2019  302:42-53  PubMed ID: 30926479
12408  Cancer therapy with major histocompatibility complex-deficient and interferon β-producing myeloid cells derived from allogeneic embryonic stem cells  Cancer therapy with major histocompatibility complex-deficient and interferon β-producing myeloid cells derived from allogeneic embryonic stem cells  Cancer Sci  2019  110(10):3027-3037  PubMed ID: 31348591
12409  Yamamoto S, Yamashita T, Ito M, Caaveiro JMM, Egashira N, Tozaki-Saitoh H, Tsuda M.  New pharmacological effect of fulvestrant to prevent oxaliplatin-induced neurodegeneration and mechanical allodynia in rats  Int J Cancer   2019  145(8):2107-2113  PubMed ID: 30515800
10546  Miyamoto H, Tsujimoto H, Horii T, Ozamoto Y, Ueda J, Takagi T, Saitoh N, Hagiwara A.  The influences of a novel anti-adhesion device, thermally cross-linked gelatin film on peritoneal dissemination of tumor cells: The in vitro and in vivo experiments using murine carcinomatous peritonitis models.  J. Biomed. Mater. Res. Part B Appl. Biomater.  2018  106(6):2122-2130  PubMed ID: 29024447
10733  Kawami M, Yamada Y, Issarachot O, Junyaprasert VB, Yumoto R, Takano M.  P-gp modulating effect of Azadirachta indica extract in multidrug-resistant cancer cell lines.  Pharmazie  2018  73:104-109  PubMed ID: 29442013
10746  Miura K, Haraguchi M, Ito H, Tai A.  Potential Antitumor Activity of 2-O-α-d-Glucopyranosyl-6-O-(2-Pentylheptanoyl)-l-Ascorbic Acid.  Int J Mol Sci  2018  19:  PubMed ID: 29439410
10772  Morio H, Sun Y, Harada M, Ide H, Shimozato O, Zhou X, Higashi K, Yuki R, Yamaguchi N, Hofbauer JP, Guttmann-Gruber C, Anzai N, Akita H, Chiba K, Furihata T.  Cancer-Type OATP1B3 mRNA in Extracellular Vesicles as a Promising Candidate for a Serum-Based Colorectal Cancer Biomarker.  Biol. Pharm. Bull.  2018  41:445-449  PubMed ID: 29491222
10827  Hatai M, Yoshikawa N, Kinoshita E, Horiyama S, Kagota S, Shinozuka K, Nakamura K.  Invasion-inhibiting Effects of Gaseous Components in Cigarette Smoke on Mouse Rectal Carcinoma Colon-26 Cells.  In Vivo  2018  32:493-497  PubMed ID: 29695551
10831  Emam SE, Ando H, Abu Lila AS, Shimizu T, Ukawa M, Okuhira K, Ishima Y, Mahdy MA, Ghazy FS, Ishida T.  A Novel Strategy to Increase the Yield of Exosomes (Extracellular Vesicles) for an Expansion of Basic Research.  Biol. Pharm. Bull.  2018  41:733-742  PubMed ID: 29709910
11060  Hayashi K, Mitsuyoshi Y, Kamei T, Shimanouchi T, Suga K, Okamoto Y, Nakamura H, Umakoshi H.  Design of Pyrene–Fatty Acid Conjugates for Real-Time Monitoring of Drug Delivery and Controllability of Drug Release  ACS Omega  2018  3:3572-3580  PubMed ID: 30023872
11199  Kuroda K, Yamashita M, Murahata Y, Azuma K, Osaki T, Tsuka T, Ito N, Imagawa T, Okamoto Y.  Use of ozonated water as a new therapeutic approach to solve current concerns around antitumor treatment.  Exp Ther Med  2018  16(3):1597-1602  PubMed ID: 30186377
11187  Noji M, So M, Yamaguchi K, Hojo H, Onda M, Akazawa-Ogawa Y, Hagihara Y, Goto Y.  Heat-Induced Aggregation of Hen Ovalbumin Suggests a Key Factor Responsible for Serpin Polymerization.  Biochemistry  2018  57(37):5415-5426  PubMed ID: 30148614
11224  Emam SE, Ando H, Lila ASA, Shimizu T, Okuhira K, Ishima Y, Mahdy MA, Ghazy FS, Sagawa I, Ishida T.  Liposome co-incubation with cancer cells secreted exosomes (extracellular vesicles) with different proteins expressions and different uptake pathways.  Sci Rep  2018    PubMed ID: 30262875
4079    Bleomycin-Loaded pH-Sensitive Polymer–Lipid-Incorporated Liposomes for Cancer Chemotherapy  Polymers  2018  10, 74 
4179  Tatebayashi D, Himori K, Yamada R, Ashida Y, Miyazaki M, Yamada T.  High-intensity eccentric training ameliorates muscle wasting in colon 26 tumor-bearing mice.  PLoS ONE  2018    PubMed ID: 29894511
10278  Miura K, Tai A.  2-O-α-D-Glucopyranosyl-l-ascorbic acid as an antitumor agent for infusion therapy.  Biochem Biophys Rep  2017  10:232-236  PubMed ID: 28955751
10424  Maeda M, Muragaki Y, Okamoto J, Yoshizawa S, Abe N, Nakamoto H, Ishii H, Kawabata K, Umemura S, Nishiyama N, Kataoka K, Iseki H.  Sonodynamic Therapy Based on Combined Use of Low Dose Administration of Epirubicin-Incorporating Drug Delivery System and Focused Ultrasound.  Ultrasound Med Biol  2017  43:2295-2301  PubMed ID: 28705555
10545  Ikemura M, Hashida T.  Effect of Hyperglycemia on Antitumor Activity and Survival in Tumor-bearing Mice Receiving Oxaliplatin and Fluorouracil.  Anticancer Res.  2017  37:5463-5468  PubMed ID: 28982857
10991  AKIKO OHKI, MINORI TANOUE, SAYUMI KOBAYASHI, KENYA MURASE  Magnetic Particle Imaging for Quantitative Evaluation of Tumor Response to Magnetic Hyperthermia Treatment Combined with Chemotherapy Using Cisplatin  Thermal Medicine  2017  33, 39-51 
11017    Comparative Study of Extracellular and Intracellular Magnetic Hyperthermia Treatments Using Magnetic Particle Imaging  Open Journal of Applied Sciences  2017  7, 647-660 
4099    A novel photodynamic therapy-based drug delivery system layered on a stent for treating cholangiocarcinoma  Biomedical Microdevices  2017  20:3  PubMed ID: 29164403
5395  Kokuryo D, Aoki I, Yuba E, Kono K, Aoshima S, Kershaw J, Saga T.  Evaluation of a combination tumor treatment using thermo-triggered liposomal drug delivery and carbon ion irradiation.  Transl Res  2017  185:24-33  PubMed ID: 28482173
7141  Vong LB, Nagasaki Y.  Combination Treatment of Murine Colon Cancer with Doxorubicin and Redox Nanoparticles.  Mol. Pharm.  2016  13:449-55  PubMed ID: 26605906
7209  Osaki T, Yokoe I, Uto Y, Ishizuka M, Tanaka T, Yamanaka N, Kurahashi T, Azuma K, Murahata Y, Tsuka T, Ito N, Imagawa T, Okamoto Y.  Bleomycin enhances the efficacy of sonodynamic therapy using aluminum phthalocyanine disulfonate.  Ultrason Sonochem  2016  28:161-168  PubMed ID: 26384895
9507  Ohyama A, Higashi T, Motoyama K, Arima H.  In Vitro and In Vivo Tumor-Targeting siRNA Delivery Using Folate-PEG-appended Dendrimer (G4)/α-Cyclodextrin Conjugates.  Bioconjug. Chem.  2016  27:521-32  PubMed ID: 26715308
9753  Gao Z, Horiguchi Y, Nakai K, Matsumura A, Suzuki M, Ono K, Nagasaki Y.  Use of boron cluster-containing redox nanoparticles with ROS scavenging ability in boron neutron capture therapy to achieve high therapeutic efficiency and low adverse effects.  Biomaterials  2016  104:201-12  PubMed ID: 27467416
9856  Kanazaki K, Sano K, Makino A, Homma T, Ono M, Saji H.  Polyoxazoline multivalently conjugated with indocyanine green for sensitive in vivo photoacoustic imaging of tumors.  Sci Rep  2016  6:33798  PubMed ID: 27667374
9995  Yamano T, Kubo S, Fukumoto M, Yano A, Mawatari-Furukawa Y, Okamura H, Tomita N.  Whole cell vaccination using immunogenic cell death by an oncolytic adenovirus is effective against a colorectal cancer model.  Mol Ther Oncolytics  2016  3:16031  PubMed ID: 28035331
10859  Tomomi Kuboyabu, Isamu Yabata, Marina Aoki, Natsuo Banura, Kohei Nishimoto, Atsushi Mimura, Kenya Murase  Magnetic Particle Imaging for Magnetic Hyperthermia Treatment: Visualization and Quantification of the Intratumoral Distribution and Temporal Change of Magnetic Nanoparticles in Vivo  Open Journal of Medical Imaging  2016  6:1-15 
10896  Tomomi Kuboyabu, Mikiko Yamawaki, Marina Aoki, Akiko Ohki, Kenya Murase   Quantitative Evaluation of Tumor Early Response to Magnetic Hyperthermia combined with Vascular Disrupting Therapy using Magnetic Particle Imaging  International Journal of Nanomedicine and Nanosurgery  2016  2.3 
12406  Akiko Ohki, Tomomi Kuboyabu, Marina Aoki, Mikiko Yamawaki, Kenya Murase  Quantitative Evaluation of Tumor Response to combination of Magnetic Hyperthermia Treatment and Radiation Therapy using Magnetic Particle Imaging  Int J Nanomed Nanosurg  2016  2.3 
12691  Suzuki R, Oda Y, Omata D, Nishiie N, Koshima R, Shiono Y, Sawaguchi Y, Unga J, Naoi T, Negishi Y, Kawakami S, Hashida M, Maruyama K.  Tumor growth suppression by the combination of nanobubbles and ultrasound  Cancer Sci  2016  107(3):217-23  PubMed ID: 26707839
7116  Hayashi K, Kiriishi M, Suga K, Okamoto Y, Umakoshi H.  Investigation of Fatty Acid Ketohydrazone Modified Liposome's Properties as a Drug Carrier.  J Drug Deliv  2015  2015:481670  PubMed ID: 26649201
7167  Kuroda K, Azuma K, Mori T, Kawamoto K, Murahata Y, Tsuka T, Osaki T, Ito N, Imagawa T, Itoh F, Okamoto Y.  The Safety and Anti-Tumor Effects of Ozonated Water in Vivo.  Int J Mol Sci  2015  16:25108-20  PubMed ID: 26506343
7195  Narayanan NK, Kunimasa K, Yamori Y, Mori M, Mori H, Nakamura K, Miller G, Manne U, Tiwari AK, Narayanan B.  Antitumor activity of melinjo (Gnetum gnemon L.) seed extract in human and murine tumor models in vitro and in a colon-26 tumor-bearing mouse model in vivo.  Cancer Med  2015  4:1767-80  PubMed ID: 26408414
7261  Aikawa T, Ito S, Shinohara M, Kaneko M, Kondo T, Yuasa M.  A drug formulation using an alginate hydrogel matrix for efficient oral delivery of the manganese porphyrin-based superoxide dismutase mimic.  Biomater Sci  2015  3:861-9  PubMed ID: 26221846
7364  Arai Y, Yamamoto T, Minamikawa T, Takamatsu T, Nagai T.  Spectral fingerprinting of individual cells visualized by cavity-reflection-enhanced light-absorption microscopy.  PLoS ONE  2015  10:e0125733  PubMed ID: 25950513
7366  Vong LB, Yoshitomi T, Matsui H, Nagasaki Y.  Development of an oral nanotherapeutics using redox nanoparticles for treatment of colitis-associated colon cancer.  Biomaterials  2015  55:54-63  PubMed ID: 25934452
7555  Ryota Araki, Shoji Nishida, Yosuke Hiraki, Takeshi Yabe  Kamikihito ameliorates tumor‐induced sickness behavior in mice.  Traditional & Kampo Medicine  2015  2:93-96 
7571  Y. Miura, A. B. Tsuji, A. Sugyo, H. Sudo, I. Aoki, M. Inubushi, M. Yashiro, K. Hirakawa, H. Cabral, N. Nishiyama, T. Saga, K. Kataoka  Polymeric Micelle Platform for Multimodal Tomographic Imaging to Detect Scirrhous Gastric Cancer.  ACS Biomater Sci Eng  2015  1:1067?1076 
12246  Kaori Miura, Futoshi Yazama, Akihiro Tai  Oxidative stress-mediated antitumor activity of erythorbic acid in high doses  Biochem Biophys Rep  2015  3:117-122  PubMed ID: 29124174
9089  Ryoko Yumoto, Saki Kakizoe, Junya Nagai, Denpong Patanasethanont, Bung-Orn Sripanidkulchai, Mikihisa Takano  Flow Cytometry Analysis of Cancer Cell Death Induced by the Extract of Thai Plant Ellipeiopsis Cherrevensis  Drug Metab Pharmacokinet  2013  28 (2), 159-63  PubMed ID: 22850758
7692  Arima H, Tsutsumi T, Yoshimatsu A, Ikeda H, Motoyama K, Higashi T, Hirayama F, Uekama K.  Inhibitory effect of siRNA complexes with polyamidoamine dendrimer/α-cyclodextrin conjugate (generation 3, G3) on endogenous gene expression.  Eur J Pharm Sci  2011  44:375-84  PubMed ID: 21896329
7811  Azuma H, Aizawa Y, Higashitani N, Tsumori T, Kojima-Yuasa A, Matsui-Yuasa I, Nagasaki T.  Biological activity of water-soluble inclusion complexes of 1'-acetoxychavicol acetate with cyclodextrins.  Bioorg. Med. Chem.  2011  19:3855-63  PubMed ID: 21596572
8283  Matsumura K, Bae JY, Hyon SH.  Polyampholytes as cryoprotective agents for mammalian cell cryopreservation.  Cell Transplant  2010  19(6):691-9  PubMed ID: 20525437

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