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Cell No. : Cell Name
RCB2652 : J774-1  update : 2023/10/16
CommentMouse cell line derived from ascites. Macrophage-like cells. BALB/c strain. TKG0208 (Deposited from Tohoku Univ.).
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor Fukuda, Hiroshi
Originator Peter, Ralph
Year of deposit 2007
Original cell TKG0208
Animal _mouse < Mammals
Strain name BALB/c
Tissue ascites
Classification transformed
History Cell Resource Center for Biomedical Research, Tohoku University(TKG0208)
Lifespan infinite
Morphology other
Cellosaurus(Expasy) CVCL_4770
deposit info
lot info
Medium Medium List
Culture type Adherent/Suspension cells
Culture medium RPMI1640 + 10% FBS
Antibiotics Free
Passage method pipetting
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
SSLP(mouse) OK
Isozyme LD, NP
deposit info
lot info
Reference information Reference 6
User's Publication 5

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1185  Sakagami H, Ikeda M, Konno K  Stimulation of tumor necrosis factor-induced human myelogenous leukemic cell differentiation by high molecular weight PSK subfraction.  Biochem Biophys Res Commun  1989  162:597-603  PubMed ID: 2757633   DOI: 10.1016/0006-291x(89)92352-8
1294  Ralph P, Nakoinz I  Direct toxic effects of immunopotentiators on monocytic, myelomonocytic, and histiocytic or macrophage tumor cells in culture.  Cancer Res  1977  37:546-50  PubMed ID: 318922  
716  Ralph P, Nakoinz I  Antibody-dependent killing of erythrocyte and tumor targets by macrophage-related cell lines: enhancement by PPD and LPS.  J Immunol  1977  119:950-54  PubMed ID: 894031  
1141  Ralph P, Moore, M A, Nilsson K  Lysozyme synthesis by established human and murine histiocytic lymphoma cell lines.  J Exp Med  1976  143:1528-33  PubMed ID: 1083890   DOI: 10.1084/jem.143.6.1528
1176  Ralph P, Prichard J, Cohn M  Reticulum cell sarcoma: an effector cell in antibody-dependent cell-mediated immunity.  J Immunol  1975  114:898-905  PubMed ID: 1089721  
1177  Ralph P, Nakoinz I  Phagocytosis and cytolysis by a macrophage tumour and its cloned cell line.  Nature  1975  257:393-4  PubMed ID: 1101071   DOI: 10.1038/257393a0

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User's Publication
17784  Saitoh Hisato, Okada Saya  Cell-derived Giant Membrane Vesicles Enclosing the Anthracycline Anti-Cancer Drug Doxorubicin and their Cytotoxicity  International Journal of Membrane Science and Technology  2022  9:13-19    DOI: 10.15379/2410-1869.2022.09.01.02
11060  Hayashi K, Mitsuyoshi Y, Kamei T, Shimanouchi T, Suga K, Okamoto Y, Nakamura H, Umakoshi H.  Design of Pyrene–Fatty Acid Conjugates for Real-Time Monitoring of Drug Delivery and Controllability of Drug Release  ACS Omega  2018  3:3572-3580  PubMed ID: 30023872   DOI: 10.1021/acsomega.7b02061
14711  Hojo K, Tamai R, Kobayashi-Sakamoto M, Kiyoura Y.  Etidronate down-regulates Toll-like receptor (TLR) 2 ligand-induced proinflammatory cytokine production by inhibiting NF-κB activation  Pharmacol Rep  2017  69(4):773-778  PubMed ID: 28587938   DOI: 10.1016/j.pharep.2017.03.009
15028  Inaba H, Yoshigai E, Okuyama T, Murakoshi M, Sugiyama K, Nishino H, Nishizawa M.  Antipyretic analgesic drugs have different mechanisms for regulation of the expression of inducible nitric oxide synthase in hepatocytes and macrophages  Nitric Oxide  2015  44:61-70  PubMed ID: 25499030   DOI: 10.1016/j.niox.2014.12.001
9145  Yuki Abiko, Keiji Nagano 1, Yasuo Yoshida 1, Fuminobu Yoshimura 1  Characterization of Treponema Denticola Mutants Defective in the Major Antigenic Proteins, Msp and TmpC  PLoS One  2014  9 (11), e113565  PubMed ID: 25401769   DOI: 10.1371/journal.pone.0113565

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