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Cell No. : Cell Name
RCB2651 : JLS-V9  update : 2023/10/16
CommentMouse cell line derived from bone marrow. Fibroblast-like cells. BALB/c strain. TKG0355 (Deposited from Tohoku Univ.).
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor Fukuda, Hiroshi
Originator Wright, B. S.
Year of deposit 2007
Original cell TKG0355
Animal _mouse < Mammals
Strain name BALB/c
Age at sampling weanling
Tissue bone marrow, tibia femur
Disease name normal
Classification other
History Cell Resource Center for Biomedical Research, Tohoku University(TKG0355)
Lifespan infinite
Morphology fibroblast-like
Cellosaurus(Expasy) CVCL_2535
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Culture medium RPMI1640 + 10% FBS
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
SSLP(mouse) OK
Isozyme LD, NP
deposit info
lot info
Reference information Reference 6
User's Publication 1

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5245  Perry A, Robinson OR Jr, Shibley GP.  Enhanced production of Rauscher leukemia virus after infection of high-passaged JLS-V9 cells.  Appl Environ Microbiol  1978  36(3):526-9  PubMed ID: 569463   DOI: 10.1128/aem.36.3.526-529.1978
5244  Johnson RW, Perry A, Robinson OR Jr, Shibley GP.  Method for reproducible large-volume production and purification of Rauscher murine leukemia virus.  Appl Environ Microbiol  1976  31(2):182-8  PubMed ID: 63267   DOI: 10.1128/aem.31.2.182-188.1976
5243  Hodge HM, Klein F, Bandyopadhyay AK, Robinson OR Jr, Shibley GP.  Adaptation and infection of mouse bone marrow (JLS-V9) cells in suspension culture for production of Rauscher leukemia virus.  Appl Microbiol  1974  27(1):224-31  PubMed ID: 4129475   DOI: 10.1128/am.27.1.224-231.1974
5242  Wright BS, O'Brien PA, Stephens R, Bielewicz H, Shibley GP.  Growth curves of Rauscher and Friend murine leukemia viruses in JLS-V9 tissue culture.  Cancer Res  1968  28(8):1655-8  PubMed ID: 5673573  
5240  Chopra HC, Shibley GP.  Infection of an established mouse cell line with cell-free Rauscher leukemia virus. I. Cytological study with the electron microscope.  J Natl Cancer Inst  1967  39(2):241-55  PubMed ID: 18623942  
5241  Wright BS, O'Brien PA, Shibley GP, Mayyasi SA, Lasfargues JC.  Infection of an established mouse bone marrow cell line (JLS-V9) with Rauscher and Moloney murine leukemia viruses.  Cancer Res  1967  27(9):1672-7  PubMed ID: 6057678  

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User's Publication
15867  Yorita N, Yuge R, Takigawa H, Ono A, Kuwai T, Kuraoka K, Kitadai Y, Tanaka S, Chayama K.  Stromal reaction inhibitor and immune-checkpoint inhibitor combination therapy attenuates excluded-type colorectal cancer in a mouse model  Cancer Lett   2021  498:111-120  PubMed ID: 33129954   DOI: 10.1016/j.canlet.2020.10.041

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