細胞番号 : 細胞名 RCB2649 : B16F1  update : 2024/10/04
細胞特性(Comment:英)			Mouse cell line derived from melanoma. C57BL/6J strain. TKG0347 (Deposited from Tohoku Univ.).
細胞特性(日)			マウスメラノーマ由来細胞株。C57BL/6J系統。 TKG0347 (東北大学医用細胞資源センターからの寄託)。
細胞特性(寄託者記述:英)
細胞特性(寄託者記述:日)
使用条件(英)			There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
使用条件(日)			基礎的研究につきましては、使用制限はありません。商業利用や特許取得を目的とする場合は、事前に必ず理研細胞バンクに連絡をすること。
備考(英)
備考(日)
提供申込書類(英)			Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)
			Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
提供申込書類(日)			依頼書C-0001.pdf   同意書(非営利学術目的)C-0003.pdf   同意書(営利目的)C-0003p.pdf
			提供同意書は、使用機関の種類や目的に応じて、非営利学術目的 (C-XXXX) と営利目的 (C-XXXXp) の2種類があります。該当する提供同意書をご使用ください（詳細）。特許等の取得及び商業利用等は事前に必ず cellbank.brc@riken.jp までご連絡ください。
提供手数料			手数料とお支払いについてはこちらをご覧ください。
細胞基本情報		寄託者	Fukuda, Hiroshi
		樹立者	Fidler, I. J.
		寄託日	2007
		元の細胞	TKG0347
		動物種	_mouse < Mammals
		動物系統名	C57BL/6J
		採取組織	skin
		病名	melanoma
		細胞分類	cancer
		入手歴	Cell Resource Center for Biomedical Research, Tohoku University（TKG0347）
		細胞寿命	infinite
		細胞形態	epithelial-like
		Cellosaurus(Expasy)	CVCL_0158
細胞培養・検査情報			寄託時情報	ロット情報
	培地・試薬情報		培地・試薬一覧はこちらをご覧ください。
		培養形態		Adherent cells
		培地		RPMI1640 + 10% FBS
		抗生物質		Free
		継代方法		0.25% Trypsin
		継代密度		1 : 8 split
		継代・培地交換頻度		Subculture : 2 times/week
		培養最適温度		37 ℃
		二酸化炭素濃度		5 %
		凍結培地		Medium + 10% DMSO
		凍結方法		Slow freezing
		マイコプラズマ/アコレプラズマ		(-)
		マウス系統検査		OK
		アイソザイム検査		LD, NP
画像情報			寄託時情報	ロット情報
文献情報		Reference(英)	8件
		Reference(日)	0件
		利用者成果(英)	15件
		利用者成果(日)	0件

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Reference(英)

5225  Fan D, Liaw A, Denkins YM, Collins JH, Van Arsdall M, Chang JL, Chakrabarty S, Nguyen D, Kruzel E, Fidler IJ.  Type-1 transforming growth factor-beta differentially modulates tumoricidal activity of murine peritoneal macrophages against metastatic variants of the B16 murine melanoma.  J Exp Ther Oncol  2002  2(5):286-97  PubMed ID: 12416032   DOI: 10.1046/j.1359-4117.2002.01053.x

5222  Price JE1, Naito S, Fidler IJ.  Growth in an organ microenvironment as a selective process in metastasis.  Clin Exp Metastasis  1988  6(1):91-102  PubMed ID: 2961497   DOI: 10.1007/BF01580409

5220  Poste G, Doll J, Fidler IJ.  Interactions among clonal subpopulations affect stability of the metastatic phenotype in polyclonal populations of B16 melanoma cells.  Proc Natl Acad Sci U S A  1981  78(10):6226-30  PubMed ID: 6947225   DOI: 10.1073/pnas.78.10.6226

5217  Raz A, Bucana C, McLellan W, Fidler IJ.  Distribution of membrane anionic sites on B16 melanoma variants with differing lung colonising potential.  Nature  1980  284(5754):363-4  PubMed ID: 7360272   DOI: 10.1038/284363a0

5219  Poste G, Doll J, Hart IR, Fidler IJ.  In vitro selection of murine B16 melanoma variants with enhanced tissue-invasive properties.  Cancer Res  1980  40(5):1636-44  PubMed ID: 7370995

5216  Fidler IJ, Nicolson GL.  Tumor cell and host properties affecting the implantation and survival of blood-borne metastatic variants of B16 melanoma.  Isr J Med Sci  1978  14(1):38-50  PubMed ID: 632082

5213  Fidler IJ, Gersten DM, Budmen MB.  Characterization in vivo and in vitro of tumor cells selected for resistance to syngeneic lymphocyte-mediated cytotoxicity.  Cancer Res  1976  36(9 pt.1):3160-5  PubMed ID: 975082

5214  Fidler IJ, Nicolson GL.  Organ selectivity for implantation survival and growth of B16 melanoma variant tumor lines.  J Natl Cancer Inst  1976  57(5):1199-202  PubMed ID: 1003551   DOI: 10.1093/jnci/57.5.1199

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Reference(日)

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利用者成果(英)

22094  Sato K, Sato T, Hirotani R, Bam M.  Effect of combined blue light and 5-ALA on mitochondrial functions and cellular responses in B16F1 melanoma and HaCaT cells.  Cytotechnology  2024  76(6):795-816  PubMed ID: 39435424   DOI: 10.1007/s10616-024-00654-x

19590  Kazuomi Sato, Yosuke Hiraga, Yuji Yamaguchi, Setsuko Sakaki, Hiroyuki Takenaka  Anti-Melanogenic and Anti-Oxidative Effects of Nostoc verrucosum (ashitsuki) Extracts  Cosmetics  2023  10:30    DOI: 10.3390/cosmetics10010030

20602  Kanemaru H, Mizukami Y, Kaneko A, Kajihara I, Fukushima S.  A protocol for quantifying lymphocyte-mediated cytotoxicity using an impedance-based real-time cell analyzer.  STAR Protoc  2022  3(1):101128  PubMed ID: 35118432   DOI: 10.1016/j.xpro.2022.101128

21039  Sato T, Sato K.  Adverse Effect of Blue Light on DNA Integrity Is Accelerated by 5-Aminolevulinic Acid in HaCaT Human Keratinocyte Cells and B16F1 Murine Melanoma Cells.  Biology (Basel)  2022  11(12)  PubMed ID: 36552253   DOI: 10.3390/biology11121743

17482  Nishio T, Kishi R, Sato K, Sato K.  Blue light exposure enhances oxidative stress, causes DNA damage, and induces apoptosis signaling in B16F1 melanoma cells  Mutat Res Genet Toxicol Environ Mutagen  2022  883-884:503562  PubMed ID: 36462794   DOI: 10.1016/j.mrgentox.2022.503562

20086  Ito Y, Sato K.  Salicylamide Enhances Melanin Synthesis in B16F1 Melanoma Cells.  Biomol Ther (Seoul)  2021    PubMed ID: 33731492   DOI: 10.4062/biomolther.2020.222

20355  Mochizuki K, Kobayashi S, Takahashi N, Sugimoto K, Sano H, Ohara Y, Mineishi S, Zhang Y, Kikuta A.  Alloantigen-activated (AAA) CD4+ T cells reinvigorate host endogenous T cell immunity to eliminate pre-established tumors in mice.  J Exp Clin Cancer Res  2021  40(1):314  PubMed ID: 34625113   DOI: 10.1186/s13046-021-02102-6

11133  Yamaguchi H, Hiroi M, Ohmori Y.  Silencing of the interferon-inducible gene Ifi204/p204 induces resistance to interferon-γ-mediated cell growth arrest of tumor cells.  Cytokine  2019  118:80-92  PubMed ID: 30017387   DOI: 10.1016/j.cyto.2018.06.029

11698  Kazuomi Sato, Yuji Yamaguchi, Setsuko Sakaki, Hiroyuki Takenaka   Pleurochrysis carterae Hot-Water Extract Inhibits Melanogenesis in Murine Melanoma Cells  Cosmetics  2019      DOI: 10.3390/cosmetics6040060

18199  Morita T, Hayashi K.  Tumor Progression Is Mediated by Thymosin-β4 through a TGFβ/MRTF Signaling Axis  Mol Cancer Res  2018  16(5):880-893  PubMed ID: 29330296   DOI: 10.1158/1541-7786.MCR-17-0715

4350  Aoto K, Ito K, Aoki S.  Complex formation between platelet-derived growth factor receptor β and transforming growth factor β receptor regulates the differentiation of mesenchymal stem cells into cancer-associated fibroblasts.  Oncotarget  2018    PubMed ID: 30344924   DOI: 10.18632/oncotarget.26124

10038  Ito M, Minami K, Sagane Y, Watanabe T, Niwa K.  Data on melanin production in B16F1 melanoma cells in the presence of emu oil.  Data Brief  2016  9:1056-1059  PubMed ID: 27924292   DOI: 10.1016/j.dib.2016.11.039

12625  Nishio T, Usami M, Awaji M, Shinohara S, Sato K.  Dual effects of acetylsalicylic acid on ERK signaling and Mitf transcription lead to inhibition of melanogenesis  Mol Cell Biochem  2016  412(1-2):101-10  PubMed ID: 26699907   DOI: 10.1007/s11010-015-2613-x

14583  Kondo T, Tsunematsu T, Yamada A, Arakaki R, Saito M, Otsuka K, Kujiraoka S, Ushio A, Kurosawa M, Kudo Y, Ishimaru N.  Acceleration of tumor growth due to dysfunction in M1 macrophages and enhanced angiogenesis in an animal model of autoimmune disease  Lab Invest  2016  96(4):468-80  PubMed ID: 26808709   DOI: 10.1038/labinvest.2015.166

18179  Chiba S, Ikushima H, Ueki H, Yanai H, Kimura Y, Hangai S, Nishio J, Negishi H, Tamura T, Saijo S, Iwakura Y, Taniguchi T.  Recognition of tumor cells by Dectin-1 orchestrates innate immune cells for anti-tumor responses  Elife  2014  3:e04177  PubMed ID: 25149452   DOI: 10.7554/eLife.04177

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