Back Back
Cell No. : Cell Name
RCB2649 : B16F1  update : 2022/12/21
CommentMouse cell line derived from melanoma. C57BL/6J strain. TKG0347 (Deposited from Tohoku Univ.).
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor Fukuda, Hiroshi
Originator Fidler, I. J.
Year of deposit 2007
Original cell TKG0347
Animal mouse < Mammals
Strain name C57BL/6J
Tissue skin
Disease name melanoma
Classification cancer
History Cell Resource Center for Biomedical Research, Tohoku University(TKG0347)
Lifespan infinite
Morphology epithelial-like
Cellosaurus(Expasy) CVCL_0158
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives RPMI1640 + 10% FBS
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
SSLP(mouse) OK
Isozyme LD, NP
deposit info
lot info
Reference information Reference 9
User's Publication 10

To topTop
17482  Nishio T, Kishi R, Sato K, Sato K.  Blue light exposure enhances oxidative stress, causes DNA damage, and induces apoptosis signaling in B16F1 melanoma cells  Mutat Res Genet Toxicol Environ Mutagen  2022  883-884:503562  PubMed ID: 36462794   DOI: 10.1016/j.mrgentox.2022.503562
5225  Fan D, Liaw A, Denkins YM, Collins JH, Van Arsdall M, Chang JL, Chakrabarty S, Nguyen D, Kruzel E, Fidler IJ.  Type-1 transforming growth factor-beta differentially modulates tumoricidal activity of murine peritoneal macrophages against metastatic variants of the B16 murine melanoma.  J Exp Ther Oncol  2002  2(5):286-97  PubMed ID: 12416032   DOI: 10.1046/j.1359-4117.2002.01053.x
5222  Price JE1, Naito S, Fidler IJ.  Growth in an organ microenvironment as a selective process in metastasis.  Clin Exp Metastasis  1988  6(1):91-102  PubMed ID: 2961497   DOI: 10.1007/BF01580409
5220  Poste G, Doll J, Fidler IJ.  Interactions among clonal subpopulations affect stability of the metastatic phenotype in polyclonal populations of B16 melanoma cells.  Proc Natl Acad Sci U S A  1981  78(10):6226-30  PubMed ID: 6947225   DOI: 10.1073/pnas.78.10.6226
5217  Raz A, Bucana C, McLellan W, Fidler IJ.  Distribution of membrane anionic sites on B16 melanoma variants with differing lung colonising potential.  Nature  1980  284(5754):363-4  PubMed ID: 7360272   DOI: 10.1038/284363a0
5219  Poste G, Doll J, Hart IR, Fidler IJ.  In vitro selection of murine B16 melanoma variants with enhanced tissue-invasive properties.  Cancer Res  1980  40(5):1636-44  PubMed ID: 7370995  
5216  Fidler IJ, Nicolson GL.  Tumor cell and host properties affecting the implantation and survival of blood-borne metastatic variants of B16 melanoma.  Isr J Med Sci  1978  14(1):38-50  PubMed ID: 632082  
5213  Fidler IJ, Gersten DM, Budmen MB.  Characterization in vivo and in vitro of tumor cells selected for resistance to syngeneic lymphocyte-mediated cytotoxicity.  Cancer Res  1976  36(9 pt.1):3160-5  PubMed ID: 975082  
5214  Fidler IJ, Nicolson GL.  Organ selectivity for implantation survival and growth of B16 melanoma variant tumor lines.  J Natl Cancer Inst  1976  57(5):1199-202  PubMed ID: 1003551   DOI: 10.1093/jnci/57.5.1199

To topTop
User's Publication
20602  Kanemaru H, Mizukami Y, Kaneko A, Kajihara I, Fukushima S.  A protocol for quantifying lymphocyte-mediated cytotoxicity using an impedance-based real-time cell analyzer.  STAR Protoc  2022  3(1):101128  PubMed ID: 35118432   DOI: 10.1016/j.xpro.2022.101128
21039  Sato T, Sato K.  Adverse Effect of Blue Light on DNA Integrity Is Accelerated by 5-Aminolevulinic Acid in HaCaT Human Keratinocyte Cells and B16F1 Murine Melanoma Cells.  Biology (Basel)  2022  11(12)  PubMed ID: 36552253   DOI: 10.3390/biology11121743
20086  Ito Y, Sato K.  Salicylamide Enhances Melanin Synthesis in B16F1 Melanoma Cells.  Biomol Ther (Seoul)  2021    PubMed ID: 33731492   DOI: 10.4062/biomolther.2020.222
20355  Mochizuki K, Kobayashi S, Takahashi N, Sugimoto K, Sano H, Ohara Y, Mineishi S, Zhang Y, Kikuta A.  Alloantigen-activated (AAA) CD4+ T cells reinvigorate host endogenous T cell immunity to eliminate pre-established tumors in mice.  J Exp Clin Cancer Res  2021  40(1):314  PubMed ID: 34625113   DOI: 10.1186/s13046-021-02102-6
11133  Yamaguchi H, Hiroi M, Ohmori Y.  Silencing of the interferon-inducible gene Ifi204/p204 induces resistance to interferon-γ-mediated cell growth arrest of tumor cells.  Cytokine  2019  118:80-92  PubMed ID: 30017387   DOI: 10.1016/j.cyto.2018.06.029
11698  Kazuomi Sato, Yuji Yamaguchi, Setsuko Sakaki, Hiroyuki Takenaka   Pleurochrysis carterae Hot-Water Extract Inhibits Melanogenesis in Murine Melanoma Cells  Cosmetics  2019      DOI: 10.3390/cosmetics6040060
4350  Aoto K, Ito K, Aoki S.  Complex formation between platelet-derived growth factor receptor β and transforming growth factor β receptor regulates the differentiation of mesenchymal stem cells into cancer-associated fibroblasts.  Oncotarget  2018    PubMed ID: 30344924   DOI: 10.18632/oncotarget.26124
10038  Ito M, Minami K, Sagane Y, Watanabe T, Niwa K.  Data on melanin production in B16F1 melanoma cells in the presence of emu oil.  Data Brief  2016  9:1056-1059  PubMed ID: 27924292   DOI: 10.1016/j.dib.2016.11.039
12625  Nishio T, Usami M, Awaji M, Shinohara S, Sato K.  Dual effects of acetylsalicylic acid on ERK signaling and Mitf transcription lead to inhibition of melanogenesis  Mol Cell Biochem  2016  412(1-2):101-10  PubMed ID: 26699907   DOI: 10.1007/s11010-015-2613-x
14583  Kondo T, Tsunematsu T, Yamada A, Arakaki R, Saito M, Otsuka K, Kujiraoka S, Ushio A, Kurosawa M, Kudo Y, Ishimaru N.  Acceleration of tumor growth due to dysfunction in M1 macrophages and enhanced angiogenesis in an animal model of autoimmune disease  Lab Invest  2016  96(4):468-80  PubMed ID: 26808709   DOI: 10.1038/labinvest.2015.166

Back Back Return Top Page