Cell No. : Cell Name
RCB2648 : D4
update : 2023/10/16
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| Comment | A subline of the NIH/3T3 cell line. NIH/3T3 cell line transfected with wild type c-erb B2 gene with carboxyl-terminal deletion. Swiss strain.TKG0300(Deposited from Tohoku Univ.). TKG0300 (Deposited from Tohoku Univ.). |
| Comment from the depositor | |
| Terms and conditions | There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC. |
| Remarks | |
| Order Form |
Order Form(C-0005.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
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|
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
|
Depositor |
Fukuda, Hiroshi
|
| Originator |
Yamamoto, T.
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| Year of deposit |
2007
|
| Original cell |
TKG0300
|
| Animal |
_mouse
< Mammals
|
| Strain name |
Swiss
|
| Age at sampling |
embryo
|
| Tissue |
embryo (NIH/3T3-derived)
|
| Disease name |
NIH/3T3 transformed cell line
|
| Classification |
transformed
|
| Recombinant |
recombinant
|
| Exogene |
wild type c-erb B2 gene
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| History |
Cell Resource Center for Biomedical Research, Tohoku University（TKG0300）
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|
Lifespan |
infinite
|
| Morphology |
other
|
| Cellosaurus(Expasy) |
CVCL_B952
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deposit info |
lot info |
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Medium |
Medium List |
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Culture type |
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Adherent cells
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| Culture medium |
|
GIT
|
| Antibiotics |
|
Free
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|
Passage method |
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0.25% Trypsin, washed once with medium
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Culture information
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Passage ratio |
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1 : 4 split
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| SC frequency |
|
Subculture : once/2 weeks, Medium Renewal : 2 times/week
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| Temperature |
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37
℃
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| CO2 concentration |
|
5
%
|
| Freeze medium |
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GIT : 2 x FM1 = 1 : 1
|
| Freezing method |
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Slow freezing
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| Mycoplasma/Acholeplasma |
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(-)
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| SSLP(mouse) |
|
OK
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| Isozyme |
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LD, NP
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| Images |
deposit info | lot info |
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| Reference information |
Reference |
3
|
| User's Publication |
0
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| Reference |
|
5231
Saga T, Endo K, Akiyama T, Sakahara H, Koizumi M, Watanabe Y, Nakai T, Hosono M, Yamamoto T, Toyoshima K, et al.
Scintigraphic detection of overexpressed c-erbB-2 protooncogene products by a class-switched murine anti-c-erbB-2 protein monoclonal antibody.
Cancer Res
1991
51(3):990-4
PubMed ID: 1671001
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5232
Masuko T, Sugahara K, Kozono M, Otsuki S, Akiyama T, Yamamoto T, Toyoshima K, Hashimoto Y.
A murine monoclonal antibody that recognizes an extracellular domain of the human c-erbB-2 protooncogene product.
Jpn J Cancer Res
1989
80(1):10-4
PubMed ID: 2565325
DOI: 10.1111/j.1349-7006.1989.tb02237.x
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5239
Yamamoto T, Ikawa S, Akiyama T, Semba K, Nomura N, Miyajima N, Saito T, Toyoshima K.
Similarity of protein encoded by the human c-erb-B-2 gene to epidermal growth factor receptor.
Nature
1986
319(6050):230-4
PubMed ID: 3003577
DOI: 10.1038/319230a0
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