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Cell No. : Cell Name
RCB2648 : D4  update : 2021/05/20
CommentA subline of the NIH/3T3 cell line. NIH/3T3 cell line transfected with wild type c-erb B2 gene with carboxyl-terminal deletion. Swiss strain.TKG0300(Deposited from Tohoku Univ.). TKG0300 (Deposited from Tohoku Univ.).
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Fukuda, Hiroshi
Originator Yamamoto, T.
Year of deposit 2007
Original cell TKG0300
Animal mouse < Mammals
Strain name Swiss
Age at sampling embryo
Tissue embryo (NIH/3T3-derived)
Disease name NIH/3T3 transformed cell line
Classification transformed
Recombinant recombinant
Exogene wild type c-erb B2 gene
History Cell Resource Center for Biomedical Research, Tohoku University(TKG0300)
Lifespan infinite
Morphology other
Cellosaurus(Expasy) CVCL_B952
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives GIT
Antibiotics Free
Passage method 0.25% Trypsin, washed once with medium
Culture information Passage ratio 1 : 4 split
SC frequency Subculture : once/2 weeks, Medium Renewal : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium GIT : 2 x FM1 = 1 : 1
Freezing method Slow freezing
Mycoplasma (-)
SSLP(mouse) OK
Isozyme LD, NP
Images
deposit info
lot info
Reference information Reference 3
User's Publication 0


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Reference
5231  Saga T, Endo K, Akiyama T, Sakahara H, Koizumi M, Watanabe Y, Nakai T, Hosono M, Yamamoto T, Toyoshima K, et al.  Scintigraphic detection of overexpressed c-erbB-2 protooncogene products by a class-switched murine anti-c-erbB-2 protein monoclonal antibody.  Cancer Res  1991  51(3):990-4  PubMed ID: 1671001  
5232  Masuko T, Sugahara K, Kozono M, Otsuki S, Akiyama T, Yamamoto T, Toyoshima K, Hashimoto Y.  A murine monoclonal antibody that recognizes an extracellular domain of the human c-erbB-2 protooncogene product.  Jpn J Cancer Res  1989  80(1):10-4  PubMed ID: 2565325   DOI: 10.1111/j.1349-7006.1989.tb02237.x
5239  Yamamoto T, Ikawa S, Akiyama T, Semba K, Nomura N, Miyajima N, Saito T, Toyoshima K.  Similarity of protein encoded by the human c-erb-B-2 gene to epidermal growth factor receptor.  Nature  1986  319(6050):230-4  PubMed ID: 3003577   DOI: 10.1038/319230a0

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