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Cell No. : Cell Name
RCB2643 : F9  update : 2021/05/20
CommentMouse cell line derived from embryonal carcinoma. 129 strain. TKG0519 (Deposited from Tohoku Univ.). Application consideration
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Fukuda, Hiroshi
Originator Bernstine
Year of deposit 2007
Original cell TKG0519
Animal mouse < Mammals
Strain name 129
Age at sampling embryo
Tissue embryo
Disease name Embryonal carcinoma
Classification cancer
History Cell Resource Center for Biomedical Research, Tohoku University(TKG0519)
Memo_1 A culture submitted to the CRCBR, Tohoku University was found to be contaminated with mycoplasma. Mycoplasma was eliminated with BM Cycline and MC210 at the CRCBR.
Lifespan infinite
Morphology other
Cellosaurus(Expasy) CVCL_0259
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives DMEM (low glucose) + 15% FBS
Antibiotics Free
Passage method pipetting or 0.25% Trypsin
Culture information Passage ratio 1 : 6 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
SSLP(mouse) OK
Isozyme LD, NP
Reference information Reference 4
User's Publication 1


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Reference
2493  Strickland S, Mahdavi V.  The induction of differentiation in teratocarcinoma stem cells by retinoic acid.  Cell  1978  15(2):393-403  PubMed ID: 214238   DOI: 10.1016/0092-8674(78)90008-9
4967  Nicolas JF, Dubois P, Jakob H, Gaillard J, Jacob F.  Mouse teratocarcinoma: differentiation in cultures of a multipotential primitive cell line (author's transl)  Ann Microbiol (Paris)  1975  126(1):3-22  PubMed ID: 1103673  
844  Berstine EG, Hooper ML, Grandchamp S, Ephrussi B.  Alkaline phosphatase activity in mouse teratoma.  Proc Natl Acad Sci U S A  1973  70(12):3899-903  PubMed ID: 4521215   DOI: 10.1073/pnas.70.12.3899
4966  Artzt K, Dubois P, Bennett D, Condamine H, Babinet C, Jacob F.  Surface antigens common to mouse cleavage embryos and primitive teratocarcinoma cells in culture.  Proc Natl Acad Sci U S A  1973  70(10):2988-92  PubMed ID: 4355379   DOI: 10.1073/pnas.70.10.2988

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User's Publication
15060  Yamada S., Ikkyu K., Iso K., Goto M., Endo  Facile synthesis of polymethionine oxides through polycondensation of activated urethane derivative of α-amino acid and their application to antifouling polymer against proteins and cells.  Polym Chem  2015  6:1838-1845    DOI: 10.1039/C4PY01656D



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