Cell No. : Cell Name
RCB2643 : F9 update : 2023/10/16 |
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Comment | Mouse cell line derived from embryonal carcinoma. 129 strain. TKG0519 (Deposited from Tohoku Univ.). Application consideration | |||
Comment from the depositor | ||||
Terms and conditions | There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC. | |||
Remarks | ||||
Order Form | Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)   | |||
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp). | ||||
Basic information | Depositor | Fukuda, Hiroshi | ||
Originator | Bernstine | |||
Year of deposit | 2007 | |||
Original cell | TKG0519 | |||
Animal | _mouse < Mammals | |||
Strain name | 129 | |||
Age at sampling | embryo | |||
Tissue | embryo | |||
Disease name | Embryonal carcinoma | |||
Classification | cancer | |||
History | Cell Resource Center for Biomedical Research, Tohoku University(TKG0519) | |||
Memo_1 | A culture submitted to the CRCBR, Tohoku University was found to be contaminated with mycoplasma. Mycoplasma was eliminated with BM Cycline and MC210 at the CRCBR. | Lifespan | infinite | |
Morphology | other | |||
Cellosaurus(Expasy) | CVCL_0259 | |||
Medium | Medium List | Culture type | Adherent cells | |
Culture medium | DMEM (low glucose) + 15% FBS | |||
Antibiotics | Free | Passage method | pipetting or 0.25% Trypsin | Culture information | Passage ratio | 1 : 6 split |
SC frequency | Subculture : 2 times/week | |||
Temperature | 37 ℃ | |||
CO2 concentration | 5 % | |||
Freeze medium | Medium + 10% DMSO | |||
Freezing method | Slow freezing | |||
Mycoplasma | (-) | |||
SSLP(mouse) | OK | |||
Isozyme | LD, NP | |||
Reference information | Reference | 6 | ||
User's Publication | 1 | |||