Cell No. : Cell Name RCB2643 : F9  update : 2023/10/16
Comment			Mouse cell line derived from embryonal carcinoma. 129 strain. TKG0519 (Deposited from Tohoku Univ.). Application consideration
Comment from the depositor
Terms and conditions			There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form			Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)
			Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
	Basic information	Depositor	Fukuda, Hiroshi
		Originator	Bernstine
		Year of deposit	2007
		Original cell	TKG0519
		Animal	_mouse < Mammals
		Strain name	129
		Age at sampling	embryo
		Tissue	embryo
		Disease name	Embryonal carcinoma
		Classification	cancer
		History	Cell Resource Center for Biomedical Research, Tohoku University（TKG0519）
		Memo_1	A culture submitted to the CRCBR, Tohoku University was found to be contaminated with mycoplasma. Mycoplasma was eliminated with BM Cycline and MC210 at the CRCBR.
		Lifespan	infinite
		Morphology	other
		Cellosaurus(Expasy)	CVCL_0259
			deposit info	lot info
		Medium	Medium List
		Culture type		Adherent cells
		Culture medium		DMEM (low glucose) + 15% FBS
		Antibiotics		Free
		Passage method		pipetting or 0.25% Trypsin
	Culture information	Passage ratio		1 : 6 split
		SC frequency		Subculture : 2 times/week
		Temperature		37 ℃
		CO2 concentration		5 %
		Freeze medium		Medium + 10% DMSO
		Freezing method		Slow freezing
		Mycoplasma		(-)
		SSLP(mouse)		OK
		Isozyme		LD, NP
Reference information		Reference	6
		User's Publication	1

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Reference

2593  Hogan BL, Taylor A, Adamson E.  Cell interactions modulate embryonal carcinoma cell differentiation into parietal or visceral endoderm  Nature  1981  291(5812):235-7  PubMed ID: 6164928   DOI: 10.1038/291235a0

2592  Strickland S, Smith KK, Marotti KR.  Hormonal induction of differentiation in teratocarcinoma stem cells: generation of parietal endoderm by retinoic acid and dibutyryl cAMP  Cell  1980  21(2):347-55.  PubMed ID: 6250719   DOI: 10.1016/0092-8674(80)90471-7

2493  Strickland S, Mahdavi V.  The induction of differentiation in teratocarcinoma stem cells by retinoic acid.  Cell  1978  15(2):393-403  PubMed ID: 214238   DOI: 10.1016/0092-8674(78)90008-9

4967  Nicolas JF, Dubois P, Jakob H, Gaillard J, Jacob F.  Mouse teratocarcinoma: differentiation in cultures of a multipotential primitive cell line (author's transl)  Ann Microbiol (Paris)  1975  126(1):3-22  PubMed ID: 1103673

844  Berstine EG, Hooper ML, Grandchamp S, Ephrussi B.  Alkaline phosphatase activity in mouse teratoma.  Proc Natl Acad Sci U S A  1973  70(12):3899-903  PubMed ID: 4521215   DOI: 10.1073/pnas.70.12.3899

4966  Artzt K, Dubois P, Bennett D, Condamine H, Babinet C, Jacob F.  Surface antigens common to mouse cleavage embryos and primitive teratocarcinoma cells in culture.  Proc Natl Acad Sci U S A  1973  70(10):2988-92  PubMed ID: 4355379   DOI: 10.1073/pnas.70.10.2988

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User's Publication

15060  Yamada S., Ikkyu K., Iso K., Goto M., Endo  Facile synthesis of polymethionine oxides through polycondensation of activated urethane derivative of α-amino acid and their application to antifouling polymer against proteins and cells.  Polym Chem  2015  6:1838-1845    DOI: 10.1039/C4PY01656D