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Cell No. : Cell Name
RCB2642 : MUSS  update : 2023/10/16
CommentMouse cell line derived from spontaneous malignant fibrous histiocytoma. A/J strain. TKG0497 (Deposited from Tohoku Univ.).
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Fukuda, Hiroshi
Originator Watanabe, Itaru
Year of deposit 2007
Original cell TKG0497
Animal _mouse < Mammals
Strain name A/J
Tissue left leg
Disease name Spontaneous malignant fibrous histiocytoma
Classification cancer
History Cell Resource Center for Biomedical Research, Tohoku University(TKG0497)
Lifespan infinite
Morphology fibroblast-like
Cellosaurus(Expasy) CVCL_8736
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Culture medium RPMI1640 + 10% FBS
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
SSLP(mouse) OK
Isozyme LD, NP
Images
deposit info
lot info
Reference information Reference 1
User's Publication 0


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Reference
2210  Watanabe I, Kurosawa N, Nishihira T.  Establishment and characterization of a murine cell-line derived from malignant fibrous histiocytoma of A/Jackson mouse.  Tohoku J Exp Med  1998  184(3):173-87  PubMed ID: 9591335   DOI: 10.1620/tjem.184.173

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