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Cell No. : Cell Name
RCB2640 : G-415  update : 2021/02/26
CommentHuman cell line derived from gallbladder carcinoma. TKG0642 (Deposited from Tohoku Univ.).
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Fukuda, Hiroshi
Originator Koyama, shohei
Year of deposit 2007
Original cell TKG0642
Animal _human < Mammals
Genus Homo
Species sapiens
Gender Male
Age at sampling 68 years
Tissue gallbladder
Disease name gallbladder carcinoma
Classification cancer
History Cell Resource Center for Biomedical Research, Tohoku University(TKG0642)
Lifespan infinite
Morphology epithelial-like
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives RPMI1640 + 10% FBS
Antibiotics Free
Passage method 0.25% trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
STR(human) OK
Isozyme LD, NP
Images
deposit info
lot info
Reference information Reference 3
User's Publication 17


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Reference
5238  Koyama S.  Augmented human-tumor-cytolytic activity of peripheral blood lymphocytes and cells from a mixed lymphocyte/tumor culture activated by interleukin-12 plus interleukin-2, and the phenotypic characterization of the cells in patients with advanced carcinoma.  J Cancer Res Clin Oncol  1997  123(9):478-84.  PubMed ID: 9341896
5237  Koyama S, Mukai R, Fukao K, Arimura H, Iwasaki Y, Osuga T.  Monoclonal antibody against human gallbladder carcinoma-associated antigen.  Cancer Res  1987  47(17):4667-73  PubMed ID: 3304618
5236  Koyama S, Yoshioka T, Mizushima A, Kawakita I, Yamagata S, Fukutomi H, Sakita T, Kondo I, Kikuchi M.  Establishment of a cell line (G-415) from a human gallbladder carcinoma.  Gan  1980  71(4):574-5  PubMed ID: 7429092

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User's Publication
13509  Ebata N, Fujita M, Sasagawa S, Maejima K, Okawa Y, Hatanaka Y, Mitsuhashi T, Oosawa-Tatsuguchi A, Tanaka H, Miyano S, Nakamura T, Hirano S, Nakagawa H.  Molecular Classification and Tumor Microenvironment Characterization of Gallbladder Cancer by Comprehensive Genomic and Transcriptomic Analysis  Cancers (Basel)  2021  13(4):733  PubMed ID: 33578820
11936  Yu J, Shi L, Lin W, Lu B, Zhao Y.  UCP2 promotes proliferation and chemoresistance through regulating the NF-κB/β-catenin axis and mitochondrial ROS in gallbladder cancer.  Biochem. Pharmacol.  2020    PubMed ID: 31811866
12106  García P, Rosa L, Vargas S, Weber H, Espinoza JA, Suárez F, Romero-Calvo I, Elgueta N, Rivera V, Nervi B, Obreque J, Leal P, Viñuela E, Aguayo G, Muñiz S, Sagredo A, Roa JC, Bizama C.  Hippo-YAP1 Is a Prognosis Marker and Potentially Targetable Pathway in Advanced Gallbladder Cancer.  Cancers (Basel)  2020    PubMed ID: 32218280
12131  García P, Bizama C, Rosa L, Espinoza JA, Weber H, Cerda-Infante J, Sánchez M, Montecinos VP, Lorenzo-Bermejo J, Boekstegers F, Dávila-López M, Alfaro F, Leiva-Acevedo C, Parra Z, Romero D, Kato S, Leal P, Lagos M, Roa JC.  Functional and genomic characterization of three novel cell lines derived from a metastatic gallbladder cancer tumor.  Biol. Res.  2020    PubMed ID: 32293552
13185  Pandey A, Stawiski EW, Durinck S, Gowda H, Goldstein LD, Barbhuiya MA, Schröder MS, Sreenivasamurthy SK, Kim SW, Phalke S, Suryamohan K, Lee K, Chakraborty P, Kode V, Shi X, Chatterjee A, Datta K, Khan AA, Subbannayya T, Wang J, Chaudhuri S, Gupta S, Shrivastav BR, Jaiswal BS, Poojary SS, Bhunia S, Garcia P, Bizama C, Rosa L, Kwon W, Kim H, Han Y, Yadav TD, Ramprasad VL, Chaudhuri A, Modrusan Z, Roa JC, Tiwari PK, Jang JY, Seshagiri S.  Integrated genomic analysis reveals mutated ELF3 as a potential gallbladder cancer vaccine candidate.  Nat Commun  2020    PubMed ID: 32839463
13261  Brägelmann J, Barahona Ponce C, Marcelain K, Roessler S, Goeppert B, Gallegos I, Colombo A, Sanhueza V, Morales E, Rivera MT, de Toro G, Ortega A, Müller B, Gabler F, Scherer D, Waldenberger M, Reischl E, Boekstegers F, Garate-Calderon V, Umu SU, Rounge TB, Popanda O, Lorenzo Bermejo J.  Epigenome-wide analysis of methylation changes in the sequence of gallstone disease, dysplasia, and gallbladder cancer.  Hepatology  2020    PubMed ID: 33020926
13843  Cristóbal Calderón-Reyes, Ramón Silva Pezoa, Pamela Leal, Alejandra Ribera-Fonseca, Cristina Cáceres, Ismael Riquelme, Tomas Zambrano, Daniela Peña, Miren Alberdi & Marjorie Reyes-Díaz  Anthocyanin-Rich Extracts of Calafate (Berberis microphylla G. Forst.) Fruits Decrease In Vitro Viability and Migration of Human Gastric and Gallbladder Cancer Cell Lines  Journal of Soil Science and Plant Nutrition  2020  20:1891–1903 
11395  Subbannayya T, Leal-Rojas P, Zhavoronkov A, Ozerov IV, Korzinkin M, Babu N, Radhakrishnan A, Chavan S, Raja R, Pinto SM, Patil AH, Barbhuiya MA, Kumar P, Guerrero-Preston R, Navani S, Tiwari PK, Kumar RV, Prasad TSK, Roa JC, Pandey A, Sidransky D, Gowda H, Izumchenko E, Chatterjee A.  PIM1 kinase promotes gallbladder cancer cell proliferation via inhibition of proline-rich Akt substrate of 40 kDa (PRAS40).  J Cell Commun Signal  2019    PubMed ID: 30666556
3726  Kirti Gondkar, Krishna Patel, Geeta V. Patil Okaly, Bipin Nair, Akhilesh Pandey, Harsha Gowda and Prashant Kumar  Dickkopf Homolog 3 (DKK3) Acts as a Potential Tumor Suppressor in Gallbladder Cancer  Front Oncol  2019  9:1121  PubMed ID: 31737564
11742  Qin Y, Zhou Y, Ge A, Chang L, Shi H, Fu Y, Luo Q.  Overexpression of SNORA21 suppresses tumorgenesis of gallbladder cancer in vitro and in vivo.  Biomed. Pharmacother.  2019    PubMed ID: 31401397
10170  Weber H, Valbuena JR, Barbhuiya MA, Stein S, Kunkel H, García P, Bizama C, Riquelme I, Espinoza JA, Kurtz SE, Tyner JW, Calderon JF, Corvalán AH, Grez M, Pandey A, Leal-Rojas P, Roa JC.  Small molecule inhibitor screening identifified HSP90 inhibitor 17-AAG as potential therapeutic agent for gallbladder cancer.  Oncotarget  2017  8:26169-26184  PubMed ID: 28412732
10222  Yang L, Huang S, Ma H, Wu X, Feng F.  MicroRNA-125b predicts clinical outcome and suppressed tumor proliferation and migration in human gallbladder cancer.  Tumour Biol.  2017  39:1010428317692249  PubMed ID: 28347246
3727  Yeung Y, Lau DK, Chionh F, Tran H, Tse JWT, Weickhardt AJ, Nikfarjam M, Scott AM, Tebbutt NC, Mariadason JM  K-Ras mutation and amplification status is predictive of resistance and high basal pAKT is predictive of sensitivity to everolimus in biliary tract cancer cell lines.  Mol Oncol  2017  11(9):1130-1142  PubMed ID: 28544747
9533  Tadokoro T, Morishita A, Fujihara S, Iwama H, Niki T, Fujita K, Akashi E, Mimura S, Oura K, Sakamoto T, Nomura T, Tani J, Miyoshi H, Yoneyama H, Himoto T, Hirashima M, Masaki T.  Galectin-9: An anticancer molecule for gallbladder carcinoma.  Int. J. Oncol.  2016  48:1165-74  PubMed ID: 26797414
3735  Saha SK et al  Isocitrate Dehydrogenase Mutations Confer Dasatinib Hypersensitivity and SRC Dependence in Intrahepatic Cholangiocarcinoma.  Cancer Discov  2016  6(7):727-39  PubMed ID: 27231123
7355  Saeki N, Ono H, Sakamoto H, Yoshida T.  Down-regulation of Immune-related Genes by PSCA in Gallbladder Cancer Cells Implanted into Mice.  Anticancer Res.  2015  35:2619-25  PubMed ID: 25964537
3729  Subbannayya T et al  Macrophage migration inhibitory factor - a therapeutic target in gallbladder cancer.  BMC Cancer  2015  15:843  PubMed ID: 26530123



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