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Cell No. : Cell Name
RCB2640 : G-415  update : 2022/09/16
CommentHuman cell line derived from gallbladder carcinoma. TKG0642 (Deposited from Tohoku Univ.).
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Fukuda, Hiroshi
Originator Koyama, shohei
Year of deposit 2007
Original cell TKG0642
Animal human < Mammals
Genus Homo
Species sapiens
Gender Male
Age at sampling 68 years
Tissue gallbladder
Disease name gallbladder carcinoma
Classification cancer
History Cell Resource Center for Biomedical Research, Tohoku University(TKG0642)
Lifespan infinite
Morphology epithelial-like
Cellosaurus(Expasy) CVCL_8198
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives RPMI1640 + 10% FBS
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Isozyme LD, NP
STR(human) OK
Images
deposit info
lot info
Reference information Reference 3
User's Publication 25


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Reference
5238  Koyama S.  Augmented human-tumor-cytolytic activity of peripheral blood lymphocytes and cells from a mixed lymphocyte/tumor culture activated by interleukin-12 plus interleukin-2, and the phenotypic characterization of the cells in patients with advanced carcinoma.  J Cancer Res Clin Oncol  1997  123(9):478-84.  PubMed ID: 9341896   DOI: 10.1007/bf01192201
5237  Koyama S, Mukai R, Fukao K, Arimura H, Iwasaki Y, Osuga T.  Monoclonal antibody against human gallbladder carcinoma-associated antigen.  Cancer Res  1987  47(17):4667-73  PubMed ID: 3304618  
5236  Koyama S, Yoshioka T, Mizushima A, Kawakita I, Yamagata S, Fukutomi H, Sakita T, Kondo I, Kikuchi M.  Establishment of a cell line (G-415) from a human gallbladder carcinoma.  Gan  1980  71(4):574-5  PubMed ID: 7429092  

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User's Publication
20567  Jain AP, Sambath J, Sathe G, George IA, Pandey A, Thompson EW, Kumar P.  Pan-cancer quantitation of epithelial-mesenchymal transition dynamics using parallel reaction monitoring-based targeted proteomics approach.  J Transl Med  2022  20(1):84  PubMed ID: 35148768   DOI: 10.1186/s12967-021-03227-0
13261  Brägelmann J, Barahona Ponce C, Marcelain K, Roessler S, Goeppert B, Gallegos I, Colombo A, Sanhueza V, Morales E, Rivera MT, de Toro G, Ortega A, Müller B, Gabler F, Scherer D, Waldenberger M, Reischl E, Boekstegers F, Garate-Calderon V, Umu SU, Rounge TB, Popanda O, Lorenzo Bermejo J.  Epigenome-wide analysis of methylation changes in the sequence of gallstone disease, dysplasia, and gallbladder cancer.  Hepatology  2021  73(6):2293-2310.  PubMed ID: 33020926   DOI: 10.1002/hep.31585
13509  Ebata N, Fujita M, Sasagawa S, Maejima K, Okawa Y, Hatanaka Y, Mitsuhashi T, Oosawa-Tatsuguchi A, Tanaka H, Miyano S, Nakamura T, Hirano S, Nakagawa H.  Molecular Classification and Tumor Microenvironment Characterization of Gallbladder Cancer by Comprehensive Genomic and Transcriptomic Analysis  Cancers (Basel)  2021  13(4):733  PubMed ID: 33578820   DOI: 10.3390/cancers13040733
20194  Ueta E, Tsutsumi K, Kato H, Matsushita H, Shiraha H, Fujii M, Matsumoto K, Horiguchi S, Okada H.  Extracellular vesicle-shuttled miRNAs as a diagnostic and prognostic biomarker and their potential roles in gallbladder cancer patients.  Sci Rep  2021  11(1):12298  PubMed ID: 34112884   DOI: 10.1038/s41598-021-91804-0
20381  Qin Y, Zheng Y, Huang C, Li Y, Gu M, Wu Q.  Knockdown of circSMAD2 inhibits the tumorigenesis of gallbladder cancer through binding with eIF4A3.  BMC Cancer  2021  21(1):1172  PubMed ID: 34727877   DOI: 10.1186/s12885-021-08895-1
16131  Tóth M, Wehling L, Thiess L, Rose F, Schmitt J, Weiler SME, Sticht C, De La Torre C, Rausch M, Albrecht T, Grabe N, Duwe L, Andersen JB, Köhler BC, Springfeld C, Mehrabi A, Kulu Y, Schirmacher P, Roessler S, Goeppert B, Breuhahn K.  Co-expression of YAP and TAZ associates with chromosomal instability in human cholangiocarcinoma  BMC Cancer  2021  21(1):1079  PubMed ID: 34615513   DOI: 10.1186/s12885-021-08794-5
17034  Qin Y, Zheng Y, Huang C, Li Y, Gu M, Wu Q.  Downregulation of miR-181b-5p Inhibits the Viability, Migration, and Glycolysis of Gallbladder Cancer by Upregulating PDHX Under Hypoxia  Front Oncol  2021  11:683725  PubMed ID: 34485121   DOI: 10.3389/fonc.2021.683725
11936  Yu J, Shi L, Lin W, Lu B, Zhao Y.  UCP2 promotes proliferation and chemoresistance through regulating the NF-κB/β-catenin axis and mitochondrial ROS in gallbladder cancer.  Biochem. Pharmacol.  2020    PubMed ID: 31811866   DOI: 10.1016/j.bcp.2019.113745
12106  García P, Rosa L, Vargas S, Weber H, Espinoza JA, Suárez F, Romero-Calvo I, Elgueta N, Rivera V, Nervi B, Obreque J, Leal P, Viñuela E, Aguayo G, Muñiz S, Sagredo A, Roa JC, Bizama C.  Hippo-YAP1 Is a Prognosis Marker and Potentially Targetable Pathway in Advanced Gallbladder Cancer.  Cancers (Basel)  2020    PubMed ID: 32218280   DOI: 10.3390/cancers12040778
12131  García P, Bizama C, Rosa L, Espinoza JA, Weber H, Cerda-Infante J, Sánchez M, Montecinos VP, Lorenzo-Bermejo J, Boekstegers F, Dávila-López M, Alfaro F, Leiva-Acevedo C, Parra Z, Romero D, Kato S, Leal P, Lagos M, Roa JC.  Functional and genomic characterization of three novel cell lines derived from a metastatic gallbladder cancer tumor.  Biol. Res.  2020    PubMed ID: 32293552   DOI: 10.1186/s40659-020-00282-7
13185  Pandey A, Stawiski EW, Durinck S, Gowda H, Goldstein LD, Barbhuiya MA, Schröder MS, Sreenivasamurthy SK, Kim SW, Phalke S, Suryamohan K, Lee K, Chakraborty P, Kode V, Shi X, Chatterjee A, Datta K, Khan AA, Subbannayya T, Wang J, Chaudhuri S, Gupta S, Shrivastav BR, Jaiswal BS, Poojary SS, Bhunia S, Garcia P, Bizama C, Rosa L, Kwon W, Kim H, Han Y, Yadav TD, Ramprasad VL, Chaudhuri A, Modrusan Z, Roa JC, Tiwari PK, Jang JY, Seshagiri S.  Integrated genomic analysis reveals mutated ELF3 as a potential gallbladder cancer vaccine candidate.  Nat Commun  2020    PubMed ID: 32839463   DOI: 10.1038/s41467-020-17880-4
13843  Cristóbal Calderón-Reyes, Ramón Silva Pezoa, Pamela Leal, Alejandra Ribera-Fonseca, Cristina Cáceres, Ismael Riquelme, Tomas Zambrano, Daniela Peña, Miren Alberdi & Marjorie Reyes-Díaz  Anthocyanin-Rich Extracts of Calafate (Berberis microphylla G. Forst.) Fruits Decrease In Vitro Viability and Migration of Human Gastric and Gallbladder Cancer Cell Lines  Journal of Soil Science and Plant Nutrition  2020  20:1891–1903    DOI: 10.1007/s42729-020-00260-8
13924  Qin Y, Mi W, Huang C, Li J, Zhang Y, Fu Y.  Downregulation of miR-575 Inhibits the Tumorigenesis of Gallbladder Cancer via Targeting p27 Kip1  Onco Targets Ther  2020  13:3667-3676  PubMed ID: 32431517   DOI: 10.2147/OTT.S229614
20122  Hendaoui I, Lahmar A, Campo L, Mebarki S, Bichet S, Hess D, Degen M, Kchir N, Charrada-Ben Farhat L, Hefaiedh R, Ruiz C, Terracciano LM, Tucker RP, Hendaoui L, Chiquet-Ehrismann R.  Tenascin-W Is a Novel Stromal Marker in Biliary Tract Cancers.  Front Immunol  2020  11:630139  PubMed ID: 33692777   DOI: 10.3389/fimmu.2020.630139
11395  Subbannayya T, Leal-Rojas P, Zhavoronkov A, Ozerov IV, Korzinkin M, Babu N, Radhakrishnan A, Chavan S, Raja R, Pinto SM, Patil AH, Barbhuiya MA, Kumar P, Guerrero-Preston R, Navani S, Tiwari PK, Kumar RV, Prasad TSK, Roa JC, Pandey A, Sidransky D, Gowda H, Izumchenko E, Chatterjee A.  PIM1 kinase promotes gallbladder cancer cell proliferation via inhibition of proline-rich Akt substrate of 40 kDa (PRAS40).  J Cell Commun Signal  2019    PubMed ID: 30666556   DOI: 10.1007/s12079-018-00503-5
3726  Kirti Gondkar, Krishna Patel, Geeta V. Patil Okaly, Bipin Nair, Akhilesh Pandey, Harsha Gowda and Prashant Kumar  Dickkopf Homolog 3 (DKK3) Acts as a Potential Tumor Suppressor in Gallbladder Cancer  Front Oncol  2019  9:1121  PubMed ID: 31737564   DOI: 10.3389/fonc.2019.01121
11742  Qin Y, Zhou Y, Ge A, Chang L, Shi H, Fu Y, Luo Q.  Overexpression of SNORA21 suppresses tumorgenesis of gallbladder cancer in vitro and in vivo.  Biomed. Pharmacother.  2019    PubMed ID: 31401397   DOI: 10.1016/j.biopha.2019.109266
10170  Weber H, Valbuena JR, Barbhuiya MA, Stein S, Kunkel H, García P, Bizama C, Riquelme I, Espinoza JA, Kurtz SE, Tyner JW, Calderon JF, Corvalán AH, Grez M, Pandey A, Leal-Rojas P, Roa JC.  Small molecule inhibitor screening identifified HSP90 inhibitor 17-AAG as potential therapeutic agent for gallbladder cancer.  Oncotarget  2017  8:26169-26184  PubMed ID: 28412732   DOI: 10.18632/oncotarget.15410
10222  Yang L, Huang S, Ma H, Wu X, Feng F.  MicroRNA-125b predicts clinical outcome and suppressed tumor proliferation and migration in human gallbladder cancer.  Tumour Biol.  2017  39:1010428317692249  PubMed ID: 28347246   DOI: 10.1177/1010428317692249
3727  Yeung Y, Lau DK, Chionh F, Tran H, Tse JWT, Weickhardt AJ, Nikfarjam M, Scott AM, Tebbutt NC, Mariadason JM  K-Ras mutation and amplification status is predictive of resistance and high basal pAKT is predictive of sensitivity to everolimus in biliary tract cancer cell lines.  Mol Oncol  2017  11(9):1130-1142  PubMed ID: 28544747   DOI: 10.1002/1878-0261.12078
9533  Tadokoro T, Morishita A, Fujihara S, Iwama H, Niki T, Fujita K, Akashi E, Mimura S, Oura K, Sakamoto T, Nomura T, Tani J, Miyoshi H, Yoneyama H, Himoto T, Hirashima M, Masaki T.  Galectin-9: An anticancer molecule for gallbladder carcinoma.  Int. J. Oncol.  2016  48:1165-74  PubMed ID: 26797414   DOI: 10.3892/ijo.2016.3347
3735  Saha SK et al  Isocitrate Dehydrogenase Mutations Confer Dasatinib Hypersensitivity and SRC Dependence in Intrahepatic Cholangiocarcinoma.  Cancer Discov  2016  6(7):727-39  PubMed ID: 27231123   DOI: 10.1158/2159-8290.CD-15-1442
7355  Saeki N, Ono H, Sakamoto H, Yoshida T.  Down-regulation of Immune-related Genes by PSCA in Gallbladder Cancer Cells Implanted into Mice.  Anticancer Res.  2015  35:2619-25  PubMed ID: 25964537  
3691  Weber H, Leal P, Stein S, Kunkel H, García P, Bizama C, Espinoza JA, Riquelme I, Nervi B, Araya JC, Grez M, Roa JC.  Rapamycin and WYE-354 suppress human gallbladder cancer xenografts in mice.  Oncotarget  2015  6(31):31877-88  PubMed ID: 26397134   DOI: 10.18632/oncotarget.5047
3729  Subbannayya T et al  Macrophage migration inhibitory factor - a therapeutic target in gallbladder cancer.  BMC Cancer  2015  15:843  PubMed ID: 26530123   DOI: 10.1186/s12885-015-1855-z



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