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細胞番号 : 細胞名
RCB2639 : NB2a  update : 2021/05/20
細胞特性(Comment:英)Mouse cell line derived from neuroblastoma. TKG0509 (Deposited from Tohoku Univ.).
細胞特性(日)マウス神経芽細胞腫(Neuroblastoma)由来細胞株。 TKG509 (東北大学医用細胞資源センターからの寄託)。
細胞特性(寄託者記述:英)
細胞特性(寄託者記述:日)
使用条件(英)There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
使用条件(日)基礎的研究につきましては、使用制限はありません。商業利用や特許取得を目的とする場合は、事前に必ず理研細胞バンクに連絡をすること。
備考(英)
備考(日)
提供申込書類(英) Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
提供申込書類(日) 依頼書C-0001.pdf   同意書(非営利学術目的)C-0003.pdf   同意書(営利目的)C-0003p.pdf  
提供同意書は、使用機関の種類や目的に応じて、非営利学術目的 (C-XXXX) と営利目的 (C-XXXXp) の2種類があります。該当する提供同意書をご使用ください(詳細)。特許等の取得及び商業利用等は事前に必ず(cellbank.brc@riken.jp)までご連絡ください。
提供手数料 手数料とお支払いについてはこちらをご覧ください。
細胞基本情報 寄託者 Fukuda, Hiroshi
寄託日 2007
元の細胞 TKG0509
動物種 _mouse < Mammals
採取組織 neuroblastoma
細胞分類 cancer
入手歴 Cell Resource Center for Biomedical Research, Tohoku University(TKG0509)
細胞寿命 infinite
細胞形態 other
Cellosaurus(Expasy) CVCL_0470
細胞培養・検査情報
寄託時情報
ロット情報
培地・試薬情報 培地・試薬一覧はこちらをご覧ください。
培養形態 Adherent/Suspension cells
培地 RPMI1640 + 10% FBS
抗生物質 Free
継代方法 pipetting
継代密度 1 : 8 split
継代・培地交換頻度 Subculture : 2 times/week
培養最適温度 37 ℃
二酸化炭素濃度 5 %
凍結培地 Medium + 10% DMSO
凍結方法 Slow freezing
マイコプラズマ (-)
マウス系統検査 OK
アイソザイム検査 LD, NP
画像情報
寄託時情報
ロット情報
文献情報 Reference(英) 3件
Reference(日) 0件
利用者成果(英) 27件
利用者成果(日) 0件

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Reference(英)
5233  Goshima Y1, Ohsako S, Yamauchi T.  Overexpression of Ca2+/calmodulin-dependent protein kinase II in Neuro2a and NG108-15 neuroblastoma cell lines promotes neurite outgrowth and growth cone motility.  J Neurosci  1993  13(2):559-67  PubMed ID: 8381167
5234  Zimmerman JM, Piffaretti JC.  Interaction of tetanus toxin and toxoid with cultured neuroblastoma cells. Analysis by immunofluorescence.  Naunyn Schmiedebergs Arch Pharmacol  1977  296(3):271-7  PubMed ID: 320489
5235  Narotzky R, Bondareff W.  Biogenic amines in cultured neuroblastoma and astrocytoma cells.  J Cell Biol  1974  63(1):64-70  PubMed ID: 4370924

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Reference(日)

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利用者成果(英)
12117  Hattori Y, Naito Y, Tsugawa Y, Nonaka S, Wake H, Nagasawa T, Kawaguchi A, Miyata T.  Transient microglial absence assists postmigratory cortical neurons in proper differentiation.  Nat Commun  2020    PubMed ID: 32242005
13146  Ertunc N, Sato C, Kitajima K.  Sialic acid sulfation is induced by the antibiotic treatment in mammalian cells.  Biosci Biotechnol Biochem  2020    PubMed ID: 32752946
9467  Miyaka Sugahara, Yuri Nakaoki, Ayano Yamaguchi, Kei Hashimoto, Yasunori Miyamoto  Vitronectin Is Involved in the Morphological Transition of Neurites in Retinoic Acid-Induced Neurogenesis of Neuroblastoma Cell Line Neuro2a  Neurochem Res  2019  44(7):1621-1635  PubMed ID: 30937689
9468  Kazuyo Fujita, Masaharu Somiya, Shun'ichi Kuroda, Shuji Hinuma  Induction of Lipid Droplets in Non-Macrophage Cells as Well as Macrophages by Liposomes and Exosomes  Biochem Biophys Res Commun  2019  510(1):184-190  PubMed ID: 30678809
10731  Kumeta M, Takahashi D, Takeyasu K, Yoshimura SH.  Cell type-specific suppression of mechanosensitive genes by audible sound stimulation.  PLoS ONE  2018  13:e0188764  PubMed ID: 29385174
4308  Yun YS, Noda S, Takahashi S, Takahashi Y, Inoue H.  Piperine-like alkamides from Piper nigrum induce BDNF promoter and promote neurite outgrowth in Neuro-2a cells.  J Nat Med  2018    PubMed ID: 29063362
10188  Hossain MS, Abe Y, Ali F, Youssef M, Honsho M, Fujiki Y, Katafuchi T.  Reduction of Ether-Type Glycerophospholipids, Plasmalogens, by NF-κB Signal Leading to Microglial Activation.  J. Neurosci.  2017  37:4074-4092  PubMed ID: 28292831
9497  Clyde Gorapava Puilingi, Yuta Kudo, Yuko Cho, Keiichi Konoki, Mari Yotsu-Yamashita  A New Sarasinoside Congener, Sarasinoside M 2, From a Marine Sponge Collected in the Solomon Islands  Biosci Biotechnol Biochem  2017  81(2):222-225  PubMed ID: 27760495
14727  Tanegashima K, Sato-Miyata Y, Funakoshi M, Nishito Y, Aigaki T, Hara T.  Epigenetic regulation of the glucose transporter gene Slc2a1 by β-hydroxybutyrate underlies preferential glucose supply to the brain of fasted mice  Genes Cells  2017  22(1):71-83  PubMed ID: 27935189
9802  Hane M, Kitajima K, Sato C.  Effects of intronic single nucleotide polymorphisms (iSNPs) of a polysialyltransferase, ST8SIA2 gene found in psychiatric disorders on its gene products.  Biochem. Biophys. Res. Commun.  2016  478:1123-9  PubMed ID: 27565727
9469  Shogo Saruhashi, Keiichi Konoki, Mari Yotsu-Yamashita  The Voltage-Gated Sodium Ion Channel Inhibitory Activities of a New Tetrodotoxin Analogue, 4,4a-anhydrotetrodotoxin, and Three Other Analogues Evaluated by Colorimetric Cell-Based Assay  Toxicon  2016  119:72-6  PubMed ID: 27212630
9487  Ahmed Zaki, Ahmed Ashour, Amira Mira, Asuka Kishikawa, Toshinori Nakagawa, Qinchang Zhu, Kuniyoshi Shimizu  Biological Activities of Oleanolic Acid Derivatives From Calendula Officinalis Seeds  Phytother Res  2016  30(5):835-41  PubMed ID: 26887328
12229  Noriyuki Otsuki, Takujiro Homma, Hiroki Fujiwara, Kenya Kaneko, Yasukazu Hozumi, Mototada Shichiri, Mizuki Takashima, Junitsu Ito, Tasuku Konno, Toshihiro Kurahashi, Yasukazu Yoshida, Kaoru Goto, Satoshi Fujii, Junichi Fujii   Trichloroethylene exposure aggravates behavioral abnormalities in mice that are deficient in superoxide dismutase  Regul Toxicol Pharmacol  2016  79:83-90  PubMed ID: 27166294
12890  Suzuki T, Kazuki Y, Oshimura M, Hara T.  Highly Efficient Transfer of Chromosomes to a Broad Range of Target Cells Using Chinese Hamster Ovary Cells Expressing Murine Leukemia Virus-Derived Envelope Proteins  PLoS One  2016  11(6):e0157187  PubMed ID: 27271046
14481  Morikawa TJ, Fujita H, Kitamura A, Horio T, Yamamoto J, Kinjo M, Sasaki A, Machiyama H, Yoshizawa K, Ichimura T, Imada K, Nagai T, Watanabe TM.  Dependence of fluorescent protein brightness on protein concentration in solution and enhancement of it  Sci Rep  2016  6:22342  PubMed ID: 26956628
9472  Yasuyuki Kitaura, Kana Inoue, Naoki Kato, Nahomi Matsushita, Yoshiharu Shimomura  Enhanced Oleate Uptake and Lipotoxicity Associated With Laurate  FEBS Open Bio . 2015 May 29;5:485-91  2015  5:485-91  PubMed ID: 26106523
15029  Mira A, Yamashita S, Katakura Y, Shimizu K.  In vitro neuroprotective activities of compounds from Angelica shikokiana Makino  Molecules   2015  20(3):4813-32  PubMed ID: 25786165
15305  Oh-Hashi K, Sone A, Hikiji T, Hirata Y, Vitiello M, Fedele M, Ishigaki S, Sobue G, Kiuchi K.  Transcriptional and post-transcriptional regulation of transmembrane protein 132A  Mol Cell Biochem  2015  405(1-2):291-9  PubMed ID: 25926156
9475  Kazuhiro Shiozaki, Sena Ryuzono, Naoto Matsushita, Asami Ikeda, Kazuki Takeshita, Petros Kingstone Chigwechokha, Masaharu Komatsu, Taeko Miyagi  Molecular Cloning and Biochemical Characterization of Medaka (Oryzias Latipes) Lysosomal neu4 Sialidase  Fish Physiol Biochemc  2014  40(5):1461-72  PubMed ID: 24744226
9479  Amira Mira, Akinobu Tanaka, Yumie Tateyama, Ryuichiro Kondo, Kuniyoshi Shimizu  Comparative Biological Study of Roots, Stems, Leaves, and Seeds of Angelica Shikokiana Makino  J Ethnopharmacol  2013  148(3):980-7  PubMed ID: 23769982
8021  Mori A, Yamashita S, Uchino K, Suga T, Ikeda T, Takamatsu K, Ishizaki M, Koide T, Kimura E, Mita S, Maeda Y, Hirano T, Uchino M.  Derlin-1 overexpression ameliorates mutant SOD1-induced endoplasmic reticulum stress by reducing mutant SOD1 accumulation.  Neurochem. Int.  2011  58:344-53  PubMed ID: 21185345
9481  Takahide Matsui, Takashi Itoh, Mitsunori Fukuda  Small GTPase Rab12 Regulates Constitutive Degradation of Transferrin Receptor  Trafficc  2011  12(10):1432-43  PubMed ID: 21718402
9482  Satoshi Yamashita, Akira Mori, En Kimura, Shuji Mita, Yasushi Maeda, Teruyuki Hirano, Makoto Uchino  DJ-1 Forms Complexes With Mutant SOD1 and Ameliorates Its Toxicity  J Neurochem  2010  113(4):860-70  PubMed ID: 20202083
6135  Tanaka M, Yamazaki M, Chiba K.  Neuroprotective action of genipin on tunicamycin-induced cytotoxicity in neuro2a cells.  Biol Pharm Bull  2009  32(7):1220-3  PubMed ID: 19571389
3767  Nishikawa M, Kira Y, Yabunaka Y, Inoue M.  Identification and characterization of endoplasmic reticulum-associated protein, ERp43.  Gene  2007  386(1-2):42-51  PubMed ID: 17020792
9524  Hidehiko Sugino, Tomoko Toyama, Yusuke Taguchi, Shigeyuki Esumi, Mitsuhiro Miyazaki, Takeshi Yagi  Negative and Positive Effects of an IAP-LTR on Nearby Pcdaalpha Gene Expression in the Central Nervous System and Neuroblastoma Cell Lines  Gene   2004  337:91-103  PubMed ID: 15276205
9527  Shanlou Qiao, Weihua Li, Ryoko Tsubouchi, Keiko Murakami, Masataka Yoshino  Role of Vanilloid Receptors in the Capsaicin-Mediated Induction of iNOS in PC12 Cells  Neurochem Res . 2004 Apr;29(4):687-93  2004  29(4):687-93  PubMed ID: 15098930

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