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Cell No. : Cell Name
RCB2639 : NB2a  update : 2024/09/09
CommentMouse cell line derived from neuroblastoma. TKG0509 (Deposited from Tohoku Univ.).
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Fukuda, Hiroshi
Year of deposit 2007
Original cell TKG0509
Animal _mouse < Mammals
Tissue neuroblastoma
Classification cancer
History Cell Resource Center for Biomedical Research, Tohoku University(TKG0509)
Lifespan infinite
Morphology other
Cellosaurus(Expasy) CVCL_0470
deposit info
lot info
Medium Medium List
Culture type Adherent/Suspension cells
Culture medium RPMI1640 + 10% FBS
Antibiotics Free
Passage method pipetting
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma/Achoreplasma (-)
SSLP(mouse) OK
Isozyme LD, NP
Images
deposit info
lot info
Reference information Reference 3
User's Publication 29


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Reference
5233  Goshima Y1, Ohsako S, Yamauchi T.  Overexpression of Ca2+/calmodulin-dependent protein kinase II in Neuro2a and NG108-15 neuroblastoma cell lines promotes neurite outgrowth and growth cone motility.  J Neurosci  1993  13(2):559-67  PubMed ID: 8381167   DOI: 10.1523/JNEUROSCI.13-02-00559.1993
5234  Zimmerman JM, Piffaretti JC.  Interaction of tetanus toxin and toxoid with cultured neuroblastoma cells. Analysis by immunofluorescence.  Naunyn Schmiedebergs Arch Pharmacol  1977  296(3):271-7  PubMed ID: 320489   DOI: 10.1007/bf00498693
5235  Narotzky R, Bondareff W.  Biogenic amines in cultured neuroblastoma and astrocytoma cells.  J Cell Biol  1974  63(1):64-70  PubMed ID: 4370924   DOI: 10.1083/jcb.63.1.64

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User's Publication
19597  Hatanaka A, Nakada S, Matsumoto G, Satoh K, Aketa I, Watanabe A, Hirakawa T, Tsujita T, Waku T, Kobayashi A.  The transcription factor NRF1 (NFE2L1) activates aggrephagy by inducing p62 and GABARAPL1 after proteasome inhibition to maintain proteostasis  Sci Rep  2023  13(1):14405  PubMed ID: 37658135   DOI: 10.1038/s41598-023-41492-9
12117  Hattori Y, Naito Y, Tsugawa Y, Nonaka S, Wake H, Nagasawa T, Kawaguchi A, Miyata T.  Transient microglial absence assists postmigratory cortical neurons in proper differentiation.  Nat Commun  2020    PubMed ID: 32242005   DOI: 10.1038/s41467-020-15409-3
13146  Ertunc N, Sato C, Kitajima K.  Sialic acid sulfation is induced by the antibiotic treatment in mammalian cells.  Biosci Biotechnol Biochem  2020    PubMed ID: 32752946   DOI: 10.1080/09168451.2020.1792763
9467  Miyaka Sugahara, Yuri Nakaoki, Ayano Yamaguchi, Kei Hashimoto, Yasunori Miyamoto  Vitronectin Is Involved in the Morphological Transition of Neurites in Retinoic Acid-Induced Neurogenesis of Neuroblastoma Cell Line Neuro2a  Neurochem Res  2019  44(7):1621-1635  PubMed ID: 30937689   DOI: 10.1007/s11064-019-02787-4
9468  Kazuyo Fujita, Masaharu Somiya, Shun'ichi Kuroda, Shuji Hinuma  Induction of Lipid Droplets in Non-Macrophage Cells as Well as Macrophages by Liposomes and Exosomes  Biochem Biophys Res Commun  2019  510(1):184-190  PubMed ID: 30678809   DOI: 10.1016/j.bbrc.2019.01.078
10731  Kumeta M, Takahashi D, Takeyasu K, Yoshimura SH.  Cell type-specific suppression of mechanosensitive genes by audible sound stimulation.  PLoS ONE  2018  13:e0188764  PubMed ID: 29385174   DOI: 10.1371/journal.pone.0188764
4308  Yun YS, Noda S, Takahashi S, Takahashi Y, Inoue H.  Piperine-like alkamides from Piper nigrum induce BDNF promoter and promote neurite outgrowth in Neuro-2a cells.  J Nat Med  2018    PubMed ID: 29063362   DOI: 10.1007/s11418-017-1140-3
10188  Hossain MS, Abe Y, Ali F, Youssef M, Honsho M, Fujiki Y, Katafuchi T.  Reduction of Ether-Type Glycerophospholipids, Plasmalogens, by NF-κB Signal Leading to Microglial Activation.  J. Neurosci.  2017  37:4074-4092  PubMed ID: 28292831   DOI: 10.1523/JNEUROSCI.3941-15.2017
9497  Clyde Gorapava Puilingi, Yuta Kudo, Yuko Cho, Keiichi Konoki, Mari Yotsu-Yamashita  A New Sarasinoside Congener, Sarasinoside M 2, From a Marine Sponge Collected in the Solomon Islands  Biosci Biotechnol Biochem  2017  81(2):222-225  PubMed ID: 27760495   DOI: 10.1080/09168451.2016.1246172
14727  Tanegashima K, Sato-Miyata Y, Funakoshi M, Nishito Y, Aigaki T, Hara T.  Epigenetic regulation of the glucose transporter gene Slc2a1 by β-hydroxybutyrate underlies preferential glucose supply to the brain of fasted mice  Genes Cells  2017  22(1):71-83  PubMed ID: 27935189   DOI: 10.1111/gtc.12456
9802  Hane M, Kitajima K, Sato C.  Effects of intronic single nucleotide polymorphisms (iSNPs) of a polysialyltransferase, ST8SIA2 gene found in psychiatric disorders on its gene products.  Biochem. Biophys. Res. Commun.  2016  478:1123-9  PubMed ID: 27565727   DOI: 10.1016/j.bbrc.2016.08.079
9469  Shogo Saruhashi, Keiichi Konoki, Mari Yotsu-Yamashita  The Voltage-Gated Sodium Ion Channel Inhibitory Activities of a New Tetrodotoxin Analogue, 4,4a-anhydrotetrodotoxin, and Three Other Analogues Evaluated by Colorimetric Cell-Based Assay  Toxicon  2016  119:72-6  PubMed ID: 27212630   DOI: 10.1016/j.toxicon.2016.05.012
9487  Ahmed Zaki, Ahmed Ashour, Amira Mira, Asuka Kishikawa, Toshinori Nakagawa, Qinchang Zhu, Kuniyoshi Shimizu  Biological Activities of Oleanolic Acid Derivatives From Calendula Officinalis Seeds  Phytother Res  2016  30(5):835-41  PubMed ID: 26887328   DOI: 10.1002/ptr.5589
12229  Noriyuki Otsuki, Takujiro Homma, Hiroki Fujiwara, Kenya Kaneko, Yasukazu Hozumi, Mototada Shichiri, Mizuki Takashima, Junitsu Ito, Tasuku Konno, Toshihiro Kurahashi, Yasukazu Yoshida, Kaoru Goto, Satoshi Fujii, Junichi Fujii   Trichloroethylene exposure aggravates behavioral abnormalities in mice that are deficient in superoxide dismutase  Regul Toxicol Pharmacol  2016  79:83-90  PubMed ID: 27166294   DOI: 10.1016/j.yrtph.2016.05.007
12890  Suzuki T, Kazuki Y, Oshimura M, Hara T.  Highly Efficient Transfer of Chromosomes to a Broad Range of Target Cells Using Chinese Hamster Ovary Cells Expressing Murine Leukemia Virus-Derived Envelope Proteins  PLoS One  2016  11(6):e0157187  PubMed ID: 27271046   DOI: 10.1371/journal.pone.0157187
14481  Morikawa TJ, Fujita H, Kitamura A, Horio T, Yamamoto J, Kinjo M, Sasaki A, Machiyama H, Yoshizawa K, Ichimura T, Imada K, Nagai T, Watanabe TM.  Dependence of fluorescent protein brightness on protein concentration in solution and enhancement of it  Sci Rep  2016  6:22342  PubMed ID: 26956628   DOI: 10.1038/srep22342
9472  Yasuyuki Kitaura, Kana Inoue, Naoki Kato, Nahomi Matsushita, Yoshiharu Shimomura  Enhanced Oleate Uptake and Lipotoxicity Associated With Laurate  FEBS Open Bio . 2015 May 29;5:485-91  2015  5:485-91  PubMed ID: 26106523   DOI: 10.1016/j.fob.2015.05.008
15029  Mira A, Yamashita S, Katakura Y, Shimizu K.  In vitro neuroprotective activities of compounds from Angelica shikokiana Makino  Molecules   2015  20(3):4813-32  PubMed ID: 25786165   DOI: 10.3390/molecules20034813
15305  Oh-Hashi K, Sone A, Hikiji T, Hirata Y, Vitiello M, Fedele M, Ishigaki S, Sobue G, Kiuchi K.  Transcriptional and post-transcriptional regulation of transmembrane protein 132A  Mol Cell Biochem  2015  405(1-2):291-9  PubMed ID: 25926156   DOI: 10.1007/s11010-015-2419-x
9475  Kazuhiro Shiozaki, Sena Ryuzono, Naoto Matsushita, Asami Ikeda, Kazuki Takeshita, Petros Kingstone Chigwechokha, Masaharu Komatsu, Taeko Miyagi  Molecular Cloning and Biochemical Characterization of Medaka (Oryzias Latipes) Lysosomal neu4 Sialidase  Fish Physiol Biochemc  2014  40(5):1461-72  PubMed ID: 24744226   DOI: 10.1007/s10695-014-9940-9
9479  Amira Mira, Akinobu Tanaka, Yumie Tateyama, Ryuichiro Kondo, Kuniyoshi Shimizu  Comparative Biological Study of Roots, Stems, Leaves, and Seeds of Angelica Shikokiana Makino  J Ethnopharmacol  2013  148(3):980-7  PubMed ID: 23769982   DOI: 10.1016/j.jep.2013.06.008
8021  Mori A, Yamashita S, Uchino K, Suga T, Ikeda T, Takamatsu K, Ishizaki M, Koide T, Kimura E, Mita S, Maeda Y, Hirano T, Uchino M.  Derlin-1 overexpression ameliorates mutant SOD1-induced endoplasmic reticulum stress by reducing mutant SOD1 accumulation.  Neurochem. Int.  2011  58:344-53  PubMed ID: 21185345   DOI: 10.1016/j.neuint.2010.12.010
9481  Takahide Matsui, Takashi Itoh, Mitsunori Fukuda  Small GTPase Rab12 Regulates Constitutive Degradation of Transferrin Receptor  Trafficc  2011  12(10):1432-43  PubMed ID: 21718402   DOI: 10.1111/j.1600-0854.2011.01240.x
9482  Satoshi Yamashita, Akira Mori, En Kimura, Shuji Mita, Yasushi Maeda, Teruyuki Hirano, Makoto Uchino  DJ-1 Forms Complexes With Mutant SOD1 and Ameliorates Its Toxicity  J Neurochem  2010  113(4):860-70  PubMed ID: 20202083   DOI: 10.1111/j.1471-4159.2010.06658.x
19124  Aoto M, Shinzawa K, Suzuki Y, Ohkubo N, Mitsuda N, Tsujimoto Y.  Essential role of p38 MAPK in caspase-independent, iPLA(2)-dependent cell death under hypoxia/low glucose conditions  FEBS Lett  2009  583(10):1611-8  PubMed ID: 19393651   DOI: 10.1016/j.febslet.2009.04.028
6135  Tanaka M, Yamazaki M, Chiba K.  Neuroprotective action of genipin on tunicamycin-induced cytotoxicity in neuro2a cells.  Biol Pharm Bull  2009  32(7):1220-3  PubMed ID: 19571389   DOI: 10.1248/bpb.32.1220
3767  Nishikawa M, Kira Y, Yabunaka Y, Inoue M.  Identification and characterization of endoplasmic reticulum-associated protein, ERp43.  Gene  2007  386(1-2):42-51  PubMed ID: 17020792   DOI: 10.1016/j.gene.2006.06.030
9524  Hidehiko Sugino, Tomoko Toyama, Yusuke Taguchi, Shigeyuki Esumi, Mitsuhiro Miyazaki, Takeshi Yagi  Negative and Positive Effects of an IAP-LTR on Nearby Pcdaalpha Gene Expression in the Central Nervous System and Neuroblastoma Cell Lines  Gene   2004  337:91-103  PubMed ID: 15276205   DOI: 10.1016/j.gene.2004.04.011
9527  Shanlou Qiao, Weihua Li, Ryoko Tsubouchi, Keiko Murakami, Masataka Yoshino  Role of Vanilloid Receptors in the Capsaicin-Mediated Induction of iNOS in PC12 Cells  Neurochem Res . 2004 Apr;29(4):687-93  2004  29(4):687-93  PubMed ID: 15098930   DOI: 10.1023/b:nere.0000018839.59457.5c



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