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Cell No. : Cell Name
RCB2639 : NB2a  update : 2020/09/24
CommentMouse cell line derived from neuroblastoma. TKG0509 (Deposited from Tohoku Univ.).
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, a prior written permission of the DEPOSITOR or the RIKEN BRC is necessary.
Remarks
Order Form Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use.Please use an appropriate MTA(to see).In case of Restriction "a" or "f", please contact RIKEN BRC(cellbank.brc@riken.jp) regarding any kind of for-profit use.
Basic information Depositor Fukuda, Hiroshi
Year of deposit 2007
Original cell TKG0509
Animal mouse < Mammals
Tissue neuroblastoma
Classification cancer
History Cell Resource Center for Biomedical Research, Tohoku University(TKG0509)
Lifespan infinite
Morphology other
deposit info
lot info
Medium Medium List
Culture type Adherent/Suspension cells
Medium and additives RPMI1640 + 10% FBS
Antibiotics Free
Passage method pipetting
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
SSLP(mouse) OK
Isozyme LD, NP
Images
deposit info
lot info
Reference information Reference 3
User's Publication 19


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Reference
5233  Goshima Y1, Ohsako S, Yamauchi T.  Overexpression of Ca2+/calmodulin-dependent protein kinase II in Neuro2a and NG108-15 neuroblastoma cell lines promotes neurite outgrowth and growth cone motility.  J Neurosci  1993  13(2):559-67  PubMed ID: 8381167
5234  Zimmerman JM, Piffaretti JC.  Interaction of tetanus toxin and toxoid with cultured neuroblastoma cells. Analysis by immunofluorescence.  Naunyn Schmiedebergs Arch Pharmacol  1977  296(3):271-7  PubMed ID: 320489
5235  Narotzky R, Bondareff W.  Biogenic amines in cultured neuroblastoma and astrocytoma cells.  J Cell Biol  1974  63(1):64-70  PubMed ID: 4370924

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User's Publication
12117  Hattori Y, Naito Y, Tsugawa Y, Nonaka S, Wake H, Nagasawa T, Kawaguchi A, Miyata T.  Transient microglial absence assists postmigratory cortical neurons in proper differentiation.  Nat Commun  2020    PubMed ID: 32242005
9467  Miyaka Sugahara, Yuri Nakaoki, Ayano Yamaguchi, Kei Hashimoto, Yasunori Miyamoto  Vitronectin Is Involved in the Morphological Transition of Neurites in Retinoic Acid-Induced Neurogenesis of Neuroblastoma Cell Line Neuro2a  Neurochem Res  2019  44(7):1621-1635  PubMed ID: 30937689
9468  Kazuyo Fujita, Masaharu Somiya, Shun'ichi Kuroda, Shuji Hinuma  Induction of Lipid Droplets in Non-Macrophage Cells as Well as Macrophages by Liposomes and Exosomes  Biochem Biophys Res Commun  2019  510(1):184-190  PubMed ID: 30678809
10731  Kumeta M, Takahashi D, Takeyasu K, Yoshimura SH.  Cell type-specific suppression of mechanosensitive genes by audible sound stimulation.  PLoS ONE  2018  13:e0188764  PubMed ID: 29385174
4308  Yun YS, Noda S, Takahashi S, Takahashi Y, Inoue H.  Piperine-like alkamides from Piper nigrum induce BDNF promoter and promote neurite outgrowth in Neuro-2a cells.  J Nat Med  2018    PubMed ID: 29063362
9497  Clyde Gorapava Puilingi, Yuta Kudo, Yuko Cho, Keiichi Konoki, Mari Yotsu-Yamashita  A New Sarasinoside Congener, Sarasinoside M 2, From a Marine Sponge Collected in the Solomon Islands  Biosci Biotechnol Biochem  2017  81(2):222-225  PubMed ID: 27760495
9802  Hane M, Kitajima K, Sato C.  Effects of intronic single nucleotide polymorphisms (iSNPs) of a polysialyltransferase, ST8SIA2 gene found in psychiatric disorders on its gene products.  Biochem. Biophys. Res. Commun.  2016  478:1123-9  PubMed ID: 27565727
9469  Shogo Saruhashi, Keiichi Konoki, Mari Yotsu-Yamashita  The Voltage-Gated Sodium Ion Channel Inhibitory Activities of a New Tetrodotoxin Analogue, 4,4a-anhydrotetrodotoxin, and Three Other Analogues Evaluated by Colorimetric Cell-Based Assay  Toxicon  2016  119:72-6  PubMed ID: 27212630
9487  Ahmed Zaki, Ahmed Ashour, Amira Mira, Asuka Kishikawa, Toshinori Nakagawa, Qinchang Zhu, Kuniyoshi Shimizu  Biological Activities of Oleanolic Acid Derivatives From Calendula Officinalis Seeds  Phytother Res  2016  30(5):835-41  PubMed ID: 26887328
12229  Noriyuki Otsuki, Takujiro Homma, Hiroki Fujiwara, Kenya Kaneko, Yasukazu Hozumi, Mototada Shichiri, Mizuki Takashima, Junitsu Ito, Tasuku Konno, Toshihiro Kurahashi, Yasukazu Yoshida, Kaoru Goto, Satoshi Fujii, Junichi Fujii   Trichloroethylene exposure aggravates behavioral abnormalities in mice that are deficient in superoxide dismutase  Regul Toxicol Pharmacol  2016  79:83-90  PubMed ID: 27166294
9475  Kazuhiro Shiozaki, Sena Ryuzono, Naoto Matsushita, Asami Ikeda, Kazuki Takeshita, Petros Kingstone Chigwechokha, Masaharu Komatsu, Taeko Miyagi  Molecular Cloning and Biochemical Characterization of Medaka (Oryzias Latipes) Lysosomal neu4 Sialidase  Fish Physiol Biochemc  2014  40(5):1461-72  PubMed ID: 24744226
9479  Amira Mira, Akinobu Tanaka, Yumie Tateyama, Ryuichiro Kondo, Kuniyoshi Shimizu  Comparative Biological Study of Roots, Stems, Leaves, and Seeds of Angelica Shikokiana Makino  J Ethnopharmacol  2013  148(3):980-7  PubMed ID: 23769982
8021  Mori A, Yamashita S, Uchino K, Suga T, Ikeda T, Takamatsu K, Ishizaki M, Koide T, Kimura E, Mita S, Maeda Y, Hirano T, Uchino M.  Derlin-1 overexpression ameliorates mutant SOD1-induced endoplasmic reticulum stress by reducing mutant SOD1 accumulation.  Neurochem. Int.  2011  58:344-53  PubMed ID: 21185345
9481  Takahide Matsui, Takashi Itoh, Mitsunori Fukuda  Small GTPase Rab12 Regulates Constitutive Degradation of Transferrin Receptor  Trafficc  2011  12(10):1432-43  PubMed ID: 21718402
9482  Satoshi Yamashita, Akira Mori, En Kimura, Shuji Mita, Yasushi Maeda, Teruyuki Hirano, Makoto Uchino  DJ-1 Forms Complexes With Mutant SOD1 and Ameliorates Its Toxicity  J Neurochem  2010  113(4):860-70  PubMed ID: 20202083
6135  Tanaka M, Yamazaki M, Chiba K.  Neuroprotective action of genipin on tunicamycin-induced cytotoxicity in neuro2a cells.  Biol Pharm Bull  2009  32(7):1220-3  PubMed ID: 19571389
3767  Nishikawa M, Kira Y, Yabunaka Y, Inoue M.  Identification and characterization of endoplasmic reticulum-associated protein, ERp43.  Gene  2007  386(1-2):42-51  PubMed ID: 17020792
9524  Hidehiko Sugino, Tomoko Toyama, Yusuke Taguchi, Shigeyuki Esumi, Mitsuhiro Miyazaki, Takeshi Yagi  Negative and Positive Effects of an IAP-LTR on Nearby Pcdaalpha Gene Expression in the Central Nervous System and Neuroblastoma Cell Lines  Gene   2004  337:91-103  PubMed ID: 15276205
9527  Shanlou Qiao, Weihua Li, Ryoko Tsubouchi, Keiko Murakami, Masataka Yoshino  Role of Vanilloid Receptors in the Capsaicin-Mediated Induction of iNOS in PC12 Cells  Neurochem Res . 2004 Apr;29(4):687-93  2004  29(4):687-93  PubMed ID: 15098930



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