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細胞番号 : 細胞名
RCB2638 : B16/BL6  update : 2024/01/05
細胞特性(Comment:英)Mouse cell line derived from melanoma.  TKG0598 (Deposited from Tohoku Univ.).
細胞特性(日)マウスメラノーマ由来細胞株。 TKG0598 (東北大学医用細胞資源センターからの寄託)。
使用条件(英)There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
提供申込書類(英) Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
提供申込書類(日) 依頼書C-0001.pdf   同意書(非営利学術目的)C-0003.pdf   同意書(営利目的)C-0003p.pdf  
提供同意書は、使用機関の種類や目的に応じて、非営利学術目的 (C-XXXX) と営利目的 (C-XXXXp) の2種類があります。該当する提供同意書をご使用ください(詳細)。特許等の取得及び商業利用等は事前に必ず cellbank.brc@riken.jp までご連絡ください。
提供手数料 手数料とお支払いについてはこちらをご覧ください。
細胞基本情報 寄託者 Fukuda, Hiroshi
寄託日 2007
元の細胞 TKG0598
動物種 _mouse < Mammals
動物系統名 C57BL/6
採取組織 skin
病名 melanoma
細胞分類 cancer
入手歴 Cell Resource Center for Biomedical Research, Tohoku University(TKG0598)
細胞寿命 infinite
細胞形態 epithelial-like
Cellosaurus(Expasy) CVCL_0157
培地・試薬情報 培地・試薬一覧はこちらをご覧ください。
培養形態 Adherent cells
培地 RPMI1640 + 10% FBS
抗生物質 Free
継代方法 0.25% Trypsin
継代密度 1 : 8 split
継代・培地交換頻度 Subculture : 2 times/week
培養最適温度 37 ℃
二酸化炭素濃度 5 %
凍結培地 Medium + 10% DMSO
凍結方法 Slow freezing
マイコプラズマ (-)
マウス系統検査 OK
アイソザイム検査 LD, NP
文献情報 Reference(英) 5件
Reference(日) 0件
利用者成果(英) 53件
利用者成果(日) 0件

5249  Bugelski PJ, Corwin SP, North SM, Kirsh RL, Nicolson GL, Poste G.  Macrophage content of spontaneous metastases at different stages of growth.  Cancer Res  1987  47(15):4141-5  PubMed ID: 3300960  
5248  Sheppard JR, Lester B, Doll J, Buscarino C, Gonzales E, Corwin S, Greig R, Poste G.  Biochemical regulation of adenylate cyclase in murine melanoma clones with different metastatic properties.  Int J Cancer  1986  37(5):713-22  PubMed ID: 3009332   DOI: 10.1002/ijc.2910370512
5246  Fidler IJ, Barnes Z, Fogler WE, Kirsh R, Bugelski P, Poste G.  Involvement of macrophages in the eradication of established metastases following intravenous injection of liposomes containing macrophage activators.  Cancer Res  1982  42(2):496-501  PubMed ID: 7055801  
5247  Poste G, Doll J, Brown AE, Tzeng J, Zeidman I.  Comparison of the metastatic properties of B16 melanoma clones isolated from cultured cell lines, subcutaneous tumors, and individual lung metastases.  Cancer Res  1982  42(7):2770-8  PubMed ID: 7083167  
5219  Poste G, Doll J, Hart IR, Fidler IJ.  In vitro selection of murine B16 melanoma variants with enhanced tissue-invasive properties.  Cancer Res  1980  40(5):1636-44  PubMed ID: 7370995  


17821  Inukai K, Kise K, Hayashi Y, Jia W, Muramatsu F, Okamoto N, Konishi H, Akuta K, Kidoya H, Takakura N.  Cancer apelin receptor suppresses vascular mimicry in malignant melanoma  Pathol Oncol Res  2023  29:1610867.  PubMed ID: 36776217   DOI: 10.3389/pore.2023.1610867
18108  Yamamoto A, Toba M, Takahashi Y, Takakura Y.  Pharmacokinetic Approach for the Elucidation of Elevated Plasma Small Extracellular Vesicle (sEV) Concentration in Cancer  J Pharm Sci  2023  112(7):1967-1974  PubMed ID: 37001861   DOI: 10.1016/j.xphs.2023.03.017
19606  Bao Y, Qiao Y, Choi JE, Zhang Y, Mannan R, Cheng C, He T, Zheng Y, Yu J, Gondal M, Cruz G, Grove S, Cao X, Su F, Wang R, Chang Y, Kryczek I, Cieslik M, Green MD, Zou W, Chinnaiyan AM.  Targeting the lipid kinase PIKfyve upregulates surface expression of MHC class I to augment cancer immunotherapy  Proc Natl Acad Sci U S A .  2023  120(49)  PubMed ID: 38011559   DOI: 10.1073/pnas.2314416120
21482  Arioka M, Seto-Tetsuo F, Inoue T, Miura K, Ishikane S, Igawa K, Tomooka K, Takahashi-Yanaga F, Sasaguri T.  Differentiation-inducing factor-1 reduces lipopolysaccharide-induced vascular cell adhesion molecule-1 by suppressing mTORC1-S6K signaling in vascular endothelial cells.  Life Sci  2023    PubMed ID: 37981227   DOI: 10.1016/j.lfs.2023.122278
17533  Liu W, Ota M, Tabushi M, Takahashi Y, Takakura Y.  Development of allergic rhinitis immunotherapy using antigen-loaded small extracellular vesicles  J Control Release   2022  345:433-442  PubMed ID: 35301052   DOI: 10.1016/j.jconrel.2022.03.016
17695  Aki Yamamoto, Yuki Takahashi, Shinsuke Inuki, Shumpei Nakagawa, Kodai Nakao, Hiroaki Ohno, Masao Doi, Yoshinobu Takakura  The identification of novel small extracellular vesicle (sEV) production modulators using luciferase‐based sEV quantification method  Journal of Extracellular Biology  2022  1    DOI: 10.1002/jex2.62
20667  Hazekawa M, Nishinakagawa T, Hosokawa M, Ishibashi D.  Development of an Organ-Directed Exosome-Based siRNA-Carrier Derived from Autologous Serum for Lung Metastases and Testing in the B16/BL6 Spontaneous Lung Metastasis Model.  Pharmaceutics  2022  14(4)  PubMed ID: 35456649   DOI: 10.3390/pharmaceutics14040815
20877  Kobayashi Y, Kitamura S, Takahashi Y, Takakura Y.  Development of Enzymatic Depletion Methods for Preparation of Small Extracellular Vesicles with Long Blood-Circulation Half-Life.  Pharm Res  2022    PubMed ID: 36195822   DOI: 10.1007/s11095-022-03405-9
14342  Ando H, Emam SE, Kawaguchi Y, Shimizu T, Ishima Y, Eshima K, Ishida T.  Increasing Tumor Extracellular pH by an Oral Alkalinizing Agent Improves Antitumor Responses of Anti-PD-1 Antibody: Implication of Relationships between Serum Bicarbonate Concentrations, Urinary pH, and Therapeutic Outcomes  Biol Pharm Bull  2021  44(6):844-852  PubMed ID: 34078817   DOI: 10.1248/bpb.b21-00076
14785  Kitai Y, Ishiura M, Saitoh K, Matsumoto N, Owashi K, Yamada S, Muromoto R, Kashiwakura JI, Oritani K, Matsuda T.  CD47 promotes T-cell lymphoma metastasis by up-regulating AKAP13-mediated RhoA activation  Int Immunol  2021  33(5):273-280  PubMed ID: 33406263   DOI: 10.1093/intimm/dxab002
20242  Mori T, Hazekawa M, Yoshida M, Nishinakagawa T, Uchida T, Ishibashi D.  Enhancing the anticancer efficacy of a LL-37 peptide fragment analog using peptide-linked PLGA conjugate micelles in tumor cells.  Int J Pharm  2021  120891  PubMed ID: 34324984   DOI: 10.1016/j.ijpharm.2021.120891
20312  Matsumoto A, Takahashi Y, Ogata K, Kitamura S, Nakagawa N, Yamamoto A, Ishihama Y, Takakura Y.  Phosphatidylserine-deficient small extracellular vesicle is a major somatic cell-derived sEV subpopulation in blood.  iScience  2021  24(8):102839  PubMed ID: 34368655   DOI: 10.1016/j.isci.2021.102839
20346  Mizukami Y, Takahashi Y, Shimizu K, Konishi S, Takakura Y, Nishikawa M.  Calcium Peroxide-Containing Polydimethylsiloxane-Based Microwells for Inhibiting Cell Death in Spheroids through Improved Oxygen Supply.  Biol Pharm Bull  2021  44(10):1458-1464  PubMed ID: 34602554   DOI: 10.1248/bpb.b21-00269
12105  Hasegawa T, Takahashi J, Nagasawa S, Doi M, Moriyama A, Iwahashi H.  DNA Strand Break Properties of Protoporphyrin IX by X-Ray Irradiation against Melanoma.  Int J Mol Sci  2020    PubMed ID: 32225109   DOI: 10.3390/ijms21072302
13029  Matsumoto A, Asuka M, Takahashi Y, Takakura Y.  Antitumor immunity by small extracellular vesicles collected from activated dendritic cells through effective induction of cellular and humoral immune responses.  Biomaterials  2020    PubMed ID: 32422494   DOI: 10.1016/j.biomaterials.2020.120112
13125  Liu W, Takahashi Y, Morishita M, Nishikawa M, Takakura Y.  Development of CD40L-modified tumor small extracellular vesicles for effective induction of antitumor immune response.  Nanomedicine (Lond)  2020    PubMed ID: 32664826   DOI: 10.2217/nnm-2020-0071
11311  Umakoshi M, Takahashi S, Itoh G, Kuriyama S, Sasaki Y, Yanagihara K, Yashiro M, Maeda D, Goto A, Tanaka M.  Macrophage-mediated transfer of cancer-derived components to stromal cells contributes to establishment of a pro-tumor microenvironment.  Oncogene  2019    PubMed ID: 30459356   DOI: 10.1038/s41388-018-0564-x
11310  Wang Y, Zenkoh J, Gerelchuluun A, Sun L, Cai S, Li X, Tsuboi K.  Administration of Dendritic Cells and Anti-PD-1 Antibody Converts X-ray Irradiated Tumors Into Effective In situ Vaccines.  Int. J. Radiat. Oncol. Biol. Phys.  2019    PubMed ID: 30458232   DOI: 10.1016/j.ijrobp.2018.11.019
11317  Yamaguchi-Sekino S, Kira T, Sekino M, Akahane M.  Effects of 7 T static magnetic fields on the expression of biological markers and the formation of bone in rats.  Bioelectromagnetics  2019    PubMed ID: 30466173   DOI: 10.1002/bem.22161
11504  Harada Y, Kizuka Y, Tokoro Y, Kondo K, Yagi H, Kato K, Inoue H, Taniguchi N, Maruyama I.  N-glycome inheritance from cells to extracellular vesicles in B16 melanomas.  FEBS Lett.  2019    PubMed ID: 30943309   DOI: 10.1002/1873-3468.13377
11822  Matsumoto A, Takahashi Y, Ariizumi R, Nishikawa M, Takakura Y.  Development of DNA-anchored assembly of small extracellular vesicle for efficient antigen delivery to antigen presenting cells.  Biomaterials  2019    PubMed ID: 31586864   DOI: 10.1016/j.biomaterials.2019.119518
11859  Emam SE, Selim Abu Lila A, Elsadek NE, Ando H, Shimizu T, Okuhira K, Ishima Y, Mahdy MA, Ghazy FS, Ishid T.  Cancer cell-type tropism is one of crucial determinants for the efficient systemic delivery of cancer cell-derived exosomes to tumor tissues.  Eur J Pharm Biopharm  2019    PubMed ID: 31629787   DOI: 10.1016/j.ejpb.2019.10.005
11926  Moriyama A, Hasegawa T, Jiang L, Iwahashi H, Mori T, Takahashi J.  Screening of X-ray responsive substances for the next generation of radiosensitizers.  Sci Rep  2019    PubMed ID: 31796788   DOI: 10.1038/s41598-019-54649-2
12390  Clemente N, Argenziano M, Gigliotti CL, Ferrara B, Boggio E, Chiocchetti A, Caldera F, Trotta F, Benetti E, Annaratone L, Ribero S, Pizzimenti S, Barrera G, Dianzani U, Cavalli R, Dianzani C.  Paclitaxel-Loaded Nanosponges Inhibit Growth and Angiogenesis in Melanoma Cell Models  Front Pharmacol  2019  10:776  PubMed ID: 31354491   DOI: 10.3389/fphar.2019.00776
10680  Arakawa N, Okubo A, Yasuhira S, Takahashi K, Amano H, Akasaka T, Masuda T, Shibazaki M, Maesawa C.  Carnosic acid, an inducer of NAD(P)H quinone oxidoreductase 1, enhances the cytotoxicity of β-lapachone in melanoma cell lines.  Oncol Lett  2018  15:2393-2400  PubMed ID: 29434949   DOI: 10.3892/ol.2017.7618
10730  Charoenviriyakul C, Takahashi Y, Morishita M, Nishikawa M, Takakura Y.  Role of Extracellular Vesicle Surface Proteins in the Pharmacokinetics of Extracellular Vesicles.  Mol. Pharm.  2018  15:1073-1080  PubMed ID: 29382201   DOI: 10.1021/acs.molpharmaceut.7b00950
10745  Takahashi J, Murakami M, Mori T, Iwahashi H.  Verification of radiodynamic therapy by medical linear accelerator using a mouse melanoma tumor model.  Sci Rep  2018  8:2728  PubMed ID: 29426920   DOI: 10.1038/s41598-018-21152-z
10805  Tahara K, Kobayashi M, Yoshida S, Onodera R, Inoue N, Takeuchi H.  Effects of cationic liposomes with stearylamine against virus infection.  Int J Pharm  2018  543:311-317  PubMed ID: 29625169   DOI: 10.1016/j.ijpharm.2018.04.001
10814  Yanaki M, Kobayashi M, Aruga A, Nomura M, Ozaki M.  In Vivo Antitumor Effects of MK615 Led by PD-L1 Downregulation.  Integr Cancer Ther  2018  17(3):646-653  PubMed ID: 29665734   DOI: 10.1177/1534735418766403
10831  Emam SE, Ando H, Abu Lila AS, Shimizu T, Ukawa M, Okuhira K, Ishima Y, Mahdy MA, Ghazy FS, Ishida T.  A Novel Strategy to Increase the Yield of Exosomes (Extracellular Vesicles) for an Expansion of Basic Research.  Biol. Pharm. Bull.  2018  41:733-742  PubMed ID: 29709910   DOI: 10.1248/bpb.b17-00919
11141  Matsumoto Y, Furusawa Y, Uzawa A, Hirayama R, Koike S, Ando K, Tsuboi K, Sakurai H.  Antimetastatic Effects of Carbon-Ion Beams on Malignant Melanomas.  Radiat. Res.  2018  190(4):412-423  PubMed ID: 30040045   DOI: 10.1667/RR15075.1
11224  Emam SE, Ando H, Lila ASA, Shimizu T, Okuhira K, Ishima Y, Mahdy MA, Ghazy FS, Sagawa I, Ishida T.  Liposome co-incubation with cancer cells secreted exosomes (extracellular vesicles) with different proteins expressions and different uptake pathways.  Sci Rep  2018    PubMed ID: 30262875   DOI: 10.1038/s41598-018-32861-w
11248  Charoenviriyakul C, Takahashi Y, Nishikawa M, Takakura Y.  Preservation of exosomes at room temperature using lyophilization.  Int J Pharm  2018    PubMed ID: 30316791   DOI: 10.1016/j.ijpharm.2018.10.032
4155  Semba T, Sugihara E, Kamoshita N, Ueno S, Fukuda K, Yoshino M, Takao K, Yoshikawa K, Izuhara K, Arima Y, Suzuki M, Saya H.  Periostin antisense oligonucleotide suppresses bleomycin-induced formation of a lung premetastatic niche for melanoma.  Cancer Sci.  2018    PubMed ID: 29498146   DOI: 10.1111/cas.13554
4155  Semba T, Sugihara E, Kamoshita N, Ueno S, Fukuda K, Yoshino M, Takao K, Yoshikawa K, Izuhara K, Arima Y, Suzuki M, Saya H.  Periostin antisense oligonucleotide suppresses bleomycin-induced formation of a lung premetastatic niche for melanoma.  Cancer Sci.  2018    PubMed ID: 29498146   DOI: 10.1111/cas.13554
4156  Hatano T, Yashiro M, Fujikawa H, Motomura H.  C-X-C Motif Ligand 1 (CXCL1) from melanoma cells down-regulates the invasion of their metastatic melanoma cells.  Oncotarget  2018    PubMed ID: 30123429   DOI: 10.18632/oncotarget.25783
10305  Arioka M, Takahashi-Yanaga F, Kubo M, Igawa K, Tomooka K, Sasaguri T.  Anti-tumor effects of differentiation-inducing factor-1 in malignant melanoma: GSK-3-mediated inhibition of cell proliferation and GSK-3-independent suppression of cell migration and invasion.  Biochem. Pharmacol.  2017  138:31-48  PubMed ID: 28501501   DOI: 10.1016/j.bcp.2017.05.004
9632  Matsumoto A, Takahashi Y, Nishikawa M, Sano K, Morishita M, Charoenviriyakul C, Saji H, Takakura Y.  Accelerated growth of B16BL6 tumor in mice through efficient uptake of their own exosomes by B16BL6 cells  Cancer Sci  2017  108(9):1803-1810  PubMed ID: 28667694   DOI: 10.1111/cas.13310
14641  Matsumoto A, Takahashi Y, Nishikawa M, Sano K, Morishita M, Charoenviriyakul C, Saji H, Takakura Y.  Charges in the Recognition and Uptake of Intravenously Injected B16BL6-Derived Exosomes by Macrophages  J Pharm Sci   2017  106(1):168-175.  PubMed ID: 27649887   DOI: 10.1016/j.xphs.2016.07.022
14710  Morishita M, Takahashi Y, Nishikawa M, Ariizumi R, Takakura Y.  Enhanced Class I Tumor Antigen Presentation via Cytosolic Delivery of Exosomal Cargos by Tumor-Cell-Derived Exosomes Displaying a pH-Sensitive Fusogenic Peptide  Mol Pharm  2017  14(11):4079-4086  PubMed ID: 28977747   DOI: 10.1021/acs.molpharmaceut.7b00760
14736  Takara K, Eino D, Ando K, Yasuda D, Naito H, Tsukada Y, Iba T, Wakabayashi T, Muramatsu F, Kidoya H, Fukuhara S, Mochizuki N, Ishii S, Kishima H, Takakura N.  Lysophosphatidic Acid Receptor 4 Activation Augments Drug Delivery in Tumors by Tightening Endothelial Cell-Cell Contact  Cell Rep  2017  20(9):2072-2086  PubMed ID: 28854359   DOI: 10.1016/j.celrep.2017.07.080
9556  Okubo A, Yasuhira S, Shibazaki M, Takahashi K, Akasaka T, Masuda T, Maesawa C.  NAD(P)H dehydrogenase, quinone 1 (NQO1), protects melanin-producing cells from cytotoxicity of rhododendrol.  Pigment Cell Melanoma Res  2016  29:309-16  PubMed ID: 26847926   DOI: 10.1111/pcmr.12461
9861  Morishita M, Takahashi Y, Matsumoto A, Nishikawa M, Takakura Y.  Exosome-based tumor antigens-adjuvant co-delivery utilizing genetically engineered tumor cell-derived exosomes with immunostimulatory CpG DNA.  Biomaterials  2016  111:55-65  PubMed ID: 27723556   DOI: 10.1016/j.biomaterials.2016.09.031
12917  Takahashi J, Misawa M, Iwahashi H.  Combined treatment with X-ray irradiation and 5-aminolevulinic acid elicits better transcriptomic response of cell cycle-related factors than X-ray irradiation alone  Int J Radiat Biol  2016  92(12):774-789  PubMed ID: 27586078   DOI: 10.1080/09553002.2016.1230240
13990  Otani K, Naito Y, Sakaguchi Y, Seo Y, Takahashi Y, Kikuta J, Ogawa K, Ishii M.  Cell-cycle-controlled radiation therapy was effective for treating a murine malignant melanoma cell line in vitro and in vivo  Sci Rep  2016  6:30689  PubMed ID: 27480052   DOI: 10.1038/srep30689
14110  Taki M, Tagami T, Fukushige K, Ozeki T.  Fabrication of nanocomposite particles using a two-solution mixing-type spray nozzle for use in an inhaled curcumin formulation  Int J Pharm  2016  511(1):104-110  PubMed ID: 27374204   DOI: 10.1016/j.ijpharm.2016.06.134
14155  Drira R, Sakamoto K.  Sakuranetin Induces Melanogenesis in B16BL6 Melanoma Cells through Inhibition of ERK and PI3K/AKT Signaling Pathways  Phytother Res  2016  30(6):997-1002  PubMed ID: 27000529   DOI: 10.1002/ptr.5606
7158  Drira R, Sakamoto K.  Isosakuranetin, a 4'-O-methylated flavonoid, stimulates melanogenesis in B16BL6 murine melanoma cells.  Life Sci.  2015  143:43-9  PubMed ID: 26524968   DOI: 10.1016/j.lfs.2015.10.009
19523  Kono Y, Kawakami S, Higuchi Y, Yamashita F, Hashida M.  In vitro evaluation of inhibitory effect of nuclear factor-kappaB activity by small interfering RNA on pro-tumor characteristics of M2-like macrophages  Biol Pharm Bull  2014  37(1):137-44  PubMed ID: 24141263   DOI: 10.1248/bpb.b13-00659
16296  Takahashi J, Misawa M, Murakami M, Mori T, Nomura K, Iwahashi H.  5-Aminolevulinic acid enhances cancer radiotherapy in a mouse tumor model  Springerplus  2013  2:602  PubMed ID: 24324921   DOI: 10.1186/2193-1801-2-602
7721  Watari K, Nakamura M, Fukunaga Y, Furuno A, Shibata T, Kawahara A, Hosoi F, Kuwano T, Kuwano M, Ono M.  The antitumor effect of a novel angiogenesis inhibitor (an octahydronaphthalene derivative) targeting both VEGF receptor and NF-κB pathway.  Int. J. Cancer  2012  131:310-21  PubMed ID: 21826646   DOI: 10.1002/ijc.26356
15745  Matsui Y, Amano H, Ito Y, Eshima K, Suzuki T, Ogawa F, Iyoda A, Satoh Y, Kato S, Nakamura M, Kitasato H, Narumiya S, Majima M.  Thromboxane A₂ receptor signaling facilitates tumor colonization through P-selectin-mediated interaction of tumor cells with platelets and endothelial cells  Cancer Sci  2012  103(4):700-7  PubMed ID: 22296266   DOI: 10.1111/j.1349-7006.2012.02200.x
7656  Fukuda H, Mochizuki S, Abe H, Okano HJ, Hara-Miyauchi C, Okano H, Yamaguchi N, Nakayama M, D'Armiento J, Okada Y.  Host-derived MMP-13 exhibits a protective role in lung metastasis of melanoma cells by local endostatin production.  Br. J. Cancer  2011  105:1615-24  PubMed ID: 22015555   DOI: 10.1038/bjc.2011.431


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