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Cell No. : Cell Name
RCB2638 : B16/BL6  update : 2021/05/20
CommentMouse cell line derived from melanoma.  TKG0598 (Deposited from Tohoku Univ.).
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Fukuda, Hiroshi
Year of deposit 2007
Original cell TKG0598
Animal mouse < Mammals
Strain name C57BL/6
Tissue skin
Disease name melanoma
Classification cancer
History Cell Resource Center for Biomedical Research, Tohoku University(TKG0598)
Lifespan infinite
Morphology epithelial-like
Cellosaurus(Expasy) CVCL_0157
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives RPMI1640 + 10% FBS
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
SSLP(mouse) OK
Isozyme LD, NP
Reference information Reference 5
User's Publication 43


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Reference
5249  Bugelski PJ, Corwin SP, North SM, Kirsh RL, Nicolson GL, Poste G.  Macrophage content of spontaneous metastases at different stages of growth.  Cancer Res  1987  47(15):4141-5  PubMed ID: 3300960
5248  Sheppard JR, Lester B, Doll J, Buscarino C, Gonzales E, Corwin S, Greig R, Poste G.  Biochemical regulation of adenylate cyclase in murine melanoma clones with different metastatic properties.  Int J Cancer  1986  37(5):713-22  PubMed ID: 3009332
5246  Fidler IJ, Barnes Z, Fogler WE, Kirsh R, Bugelski P, Poste G.  Involvement of macrophages in the eradication of established metastases following intravenous injection of liposomes containing macrophage activators.  Cancer Res  1982  42(2):496-501  PubMed ID: 7055801
5247  Poste G, Doll J, Brown AE, Tzeng J, Zeidman I.  Comparison of the metastatic properties of B16 melanoma clones isolated from cultured cell lines, subcutaneous tumors, and individual lung metastases.  Cancer Res  1982  42(7):2770-8  PubMed ID: 7083167
5219  Poste G, Doll J, Hart IR, Fidler IJ.  In vitro selection of murine B16 melanoma variants with enhanced tissue-invasive properties.  Cancer Res  1980  40(5):1636-44  PubMed ID: 7370995

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User's Publication
20667  Hazekawa M, Nishinakagawa T, Hosokawa M, Ishibashi D.  Development of an Organ-Directed Exosome-Based siRNA-Carrier Derived from Autologous Serum for Lung Metastases and Testing in the B16/BL6 Spontaneous Lung Metastasis Model.  Pharmaceutics  2022  14(4)  PubMed ID: 35456649
14342  Ando H, Emam SE, Kawaguchi Y, Shimizu T, Ishima Y, Eshima K, Ishida T.  Increasing Tumor Extracellular pH by an Oral Alkalinizing Agent Improves Antitumor Responses of Anti-PD-1 Antibody: Implication of Relationships between Serum Bicarbonate Concentrations, Urinary pH, and Therapeutic Outcomes  Biol Pharm Bull  2021  44(6):844-852  PubMed ID: 34078817
14785  Kitai Y, Ishiura M, Saitoh K, Matsumoto N, Owashi K, Yamada S, Muromoto R, Kashiwakura JI, Oritani K, Matsuda T.  CD47 promotes T-cell lymphoma metastasis by up-regulating AKAP13-mediated RhoA activation  Int Immunol  2021  33(5):273-280  PubMed ID: 33406263
20242  Mori T, Hazekawa M, Yoshida M, Nishinakagawa T, Uchida T, Ishibashi D.  Enhancing the anticancer efficacy of a LL-37 peptide fragment analog using peptide-linked PLGA conjugate micelles in tumor cells.  Int J Pharm  2021  120891  PubMed ID: 34324984
20346  Mizukami Y, Takahashi Y, Shimizu K, Konishi S, Takakura Y, Nishikawa M.  Calcium Peroxide-Containing Polydimethylsiloxane-Based Microwells for Inhibiting Cell Death in Spheroids through Improved Oxygen Supply.  Biol Pharm Bull  2021  44(10):1458-1464  PubMed ID: 34602554
12105  Hasegawa T, Takahashi J, Nagasawa S, Doi M, Moriyama A, Iwahashi H.  DNA Strand Break Properties of Protoporphyrin IX by X-Ray Irradiation against Melanoma.  Int J Mol Sci  2020    PubMed ID: 32225109
13029  Matsumoto A, Asuka M, Takahashi Y, Takakura Y.  Antitumor immunity by small extracellular vesicles collected from activated dendritic cells through effective induction of cellular and humoral immune responses.  Biomaterials  2020    PubMed ID: 32422494
13125  Liu W, Takahashi Y, Morishita M, Nishikawa M, Takakura Y.  Development of CD40L-modified tumor small extracellular vesicles for effective induction of antitumor immune response.  Nanomedicine (Lond)  2020    PubMed ID: 32664826
11311  Umakoshi M, Takahashi S, Itoh G, Kuriyama S, Sasaki Y, Yanagihara K, Yashiro M, Maeda D, Goto A, Tanaka M.  Macrophage-mediated transfer of cancer-derived components to stromal cells contributes to establishment of a pro-tumor microenvironment.  Oncogene  2019    PubMed ID: 30459356
11310  Wang Y, Zenkoh J, Gerelchuluun A, Sun L, Cai S, Li X, Tsuboi K.  Administration of Dendritic Cells and Anti-PD-1 Antibody Converts X-ray Irradiated Tumors Into Effective In situ Vaccines.  Int. J. Radiat. Oncol. Biol. Phys.  2019    PubMed ID: 30458232
11317  Yamaguchi-Sekino S, Kira T, Sekino M, Akahane M.  Effects of 7 T static magnetic fields on the expression of biological markers and the formation of bone in rats.  Bioelectromagnetics  2019    PubMed ID: 30466173
11504  Harada Y, Kizuka Y, Tokoro Y, Kondo K, Yagi H, Kato K, Inoue H, Taniguchi N, Maruyama I.  N-glycome inheritance from cells to extracellular vesicles in B16 melanomas.  FEBS Lett.  2019    PubMed ID: 30943309
11822  Matsumoto A, Takahashi Y, Ariizumi R, Nishikawa M, Takakura Y.  Development of DNA-anchored assembly of small extracellular vesicle for efficient antigen delivery to antigen presenting cells.  Biomaterials  2019    PubMed ID: 31586864
11859  Emam SE, Selim Abu Lila A, Elsadek NE, Ando H, Shimizu T, Okuhira K, Ishima Y, Mahdy MA, Ghazy FS, Ishid T.  Cancer cell-type tropism is one of crucial determinants for the efficient systemic delivery of cancer cell-derived exosomes to tumor tissues.  Eur J Pharm Biopharm  2019    PubMed ID: 31629787
11926  Moriyama A, Hasegawa T, Jiang L, Iwahashi H, Mori T, Takahashi J.  Screening of X-ray responsive substances for the next generation of radiosensitizers.  Sci Rep  2019    PubMed ID: 31796788
12390  Clemente N, Argenziano M, Gigliotti CL, Ferrara B, Boggio E, Chiocchetti A, Caldera F, Trotta F, Benetti E, Annaratone L, Ribero S, Pizzimenti S, Barrera G, Dianzani U, Cavalli R, Dianzani C.  Paclitaxel-Loaded Nanosponges Inhibit Growth and Angiogenesis in Melanoma Cell Models  Front Pharmacol  2019  10:776  PubMed ID: 31354491
10680  Arakawa N, Okubo A, Yasuhira S, Takahashi K, Amano H, Akasaka T, Masuda T, Shibazaki M, Maesawa C.  Carnosic acid, an inducer of NAD(P)H quinone oxidoreductase 1, enhances the cytotoxicity of β-lapachone in melanoma cell lines.  Oncol Lett  2018  15:2393-2400  PubMed ID: 29434949
10730  Charoenviriyakul C, Takahashi Y, Morishita M, Nishikawa M, Takakura Y.  Role of Extracellular Vesicle Surface Proteins in the Pharmacokinetics of Extracellular Vesicles.  Mol. Pharm.  2018  15:1073-1080  PubMed ID: 29382201
10745  Takahashi J, Murakami M, Mori T, Iwahashi H.  Verification of radiodynamic therapy by medical linear accelerator using a mouse melanoma tumor model.  Sci Rep  2018  8:2728  PubMed ID: 29426920
10814  Yanaki M, Kobayashi M, Aruga A, Nomura M, Ozaki M.  In Vivo Antitumor Effects of MK615 Led by PD-L1 Downregulation.  Integr Cancer Ther  2018  17(3):646-653  PubMed ID: 29665734
10831  Emam SE, Ando H, Abu Lila AS, Shimizu T, Ukawa M, Okuhira K, Ishima Y, Mahdy MA, Ghazy FS, Ishida T.  A Novel Strategy to Increase the Yield of Exosomes (Extracellular Vesicles) for an Expansion of Basic Research.  Biol. Pharm. Bull.  2018  41:733-742  PubMed ID: 29709910
11141  Matsumoto Y, Furusawa Y, Uzawa A, Hirayama R, Koike S, Ando K, Tsuboi K, Sakurai H.  Antimetastatic Effects of Carbon-Ion Beams on Malignant Melanomas.  Radiat. Res.  2018  190(4):412-423  PubMed ID: 30040045
11224  Emam SE, Ando H, Lila ASA, Shimizu T, Okuhira K, Ishima Y, Mahdy MA, Ghazy FS, Sagawa I, Ishida T.  Liposome co-incubation with cancer cells secreted exosomes (extracellular vesicles) with different proteins expressions and different uptake pathways.  Sci Rep  2018    PubMed ID: 30262875
11248  Charoenviriyakul C, Takahashi Y, Nishikawa M, Takakura Y.  Preservation of exosomes at room temperature using lyophilization.  Int J Pharm  2018    PubMed ID: 30316791
4155  Semba T, Sugihara E, Kamoshita N, Ueno S, Fukuda K, Yoshino M, Takao K, Yoshikawa K, Izuhara K, Arima Y, Suzuki M, Saya H.  Periostin antisense oligonucleotide suppresses bleomycin-induced formation of a lung premetastatic niche for melanoma.  Cancer Sci.  2018    PubMed ID: 29498146
4155  Semba T, Sugihara E, Kamoshita N, Ueno S, Fukuda K, Yoshino M, Takao K, Yoshikawa K, Izuhara K, Arima Y, Suzuki M, Saya H.  Periostin antisense oligonucleotide suppresses bleomycin-induced formation of a lung premetastatic niche for melanoma.  Cancer Sci.  2018    PubMed ID: 29498146
4156  Hatano T, Yashiro M, Fujikawa H, Motomura H.  C-X-C Motif Ligand 1 (CXCL1) from melanoma cells down-regulates the invasion of their metastatic melanoma cells.  Oncotarget  2018    PubMed ID: 30123429
10305  Arioka M, Takahashi-Yanaga F, Kubo M, Igawa K, Tomooka K, Sasaguri T.  Anti-tumor effects of differentiation-inducing factor-1 in malignant melanoma: GSK-3-mediated inhibition of cell proliferation and GSK-3-independent suppression of cell migration and invasion.  Biochem. Pharmacol.  2017  138:31-48  PubMed ID: 28501501
9632  Matsumoto A, Takahashi Y, Nishikawa M, Sano K, Morishita M, Charoenviriyakul C, Saji H, Takakura Y.  Accelerated growth of B16BL6 tumor in mice through efficient uptake of their own exosomes by B16BL6 cells  Cancer Sci  2017  108(9):1803-1810  PubMed ID: 28667694
14641  Matsumoto A, Takahashi Y, Nishikawa M, Sano K, Morishita M, Charoenviriyakul C, Saji H, Takakura Y.  Charges in the Recognition and Uptake of Intravenously Injected B16BL6-Derived Exosomes by Macrophages  J Pharm Sci   2017  106(1):168-175.  PubMed ID: 27649887
14710  Morishita M, Takahashi Y, Nishikawa M, Ariizumi R, Takakura Y.  Enhanced Class I Tumor Antigen Presentation via Cytosolic Delivery of Exosomal Cargos by Tumor-Cell-Derived Exosomes Displaying a pH-Sensitive Fusogenic Peptide  Mol Pharm  2017  14(11):4079-4086  PubMed ID: 28977747
14736  Takara K, Eino D, Ando K, Yasuda D, Naito H, Tsukada Y, Iba T, Wakabayashi T, Muramatsu F, Kidoya H, Fukuhara S, Mochizuki N, Ishii S, Kishima H, Takakura N.  Lysophosphatidic Acid Receptor 4 Activation Augments Drug Delivery in Tumors by Tightening Endothelial Cell-Cell Contact  Cell Rep  2017  20(9):2072-2086  PubMed ID: 28854359
9556  Okubo A, Yasuhira S, Shibazaki M, Takahashi K, Akasaka T, Masuda T, Maesawa C.  NAD(P)H dehydrogenase, quinone 1 (NQO1), protects melanin-producing cells from cytotoxicity of rhododendrol.  Pigment Cell Melanoma Res  2016  29:309-16  PubMed ID: 26847926
9861  Morishita M, Takahashi Y, Matsumoto A, Nishikawa M, Takakura Y.  Exosome-based tumor antigens-adjuvant co-delivery utilizing genetically engineered tumor cell-derived exosomes with immunostimulatory CpG DNA.  Biomaterials  2016  111:55-65  PubMed ID: 27723556
12917  Takahashi J, Misawa M, Iwahashi H.  Combined treatment with X-ray irradiation and 5-aminolevulinic acid elicits better transcriptomic response of cell cycle-related factors than X-ray irradiation alone  Int J Radiat Biol  2016  92(12):774-789  PubMed ID: 27586078
13990  Otani K, Naito Y, Sakaguchi Y, Seo Y, Takahashi Y, Kikuta J, Ogawa K, Ishii M.  Cell-cycle-controlled radiation therapy was effective for treating a murine malignant melanoma cell line in vitro and in vivo  Sci Rep  2016  6:30689  PubMed ID: 27480052
14110  Taki M, Tagami T, Fukushige K, Ozeki T.  Fabrication of nanocomposite particles using a two-solution mixing-type spray nozzle for use in an inhaled curcumin formulation  Int J Pharm  2016  511(1):104-110  PubMed ID: 27374204
14155  Drira R, Sakamoto K.  Sakuranetin Induces Melanogenesis in B16BL6 Melanoma Cells through Inhibition of ERK and PI3K/AKT Signaling Pathways  Phytother Res  2016  30(6):997-1002  PubMed ID: 27000529
7158  Drira R, Sakamoto K.  Isosakuranetin, a 4'-O-methylated flavonoid, stimulates melanogenesis in B16BL6 murine melanoma cells.  Life Sci.  2015  143:43-9  PubMed ID: 26524968
16296  Takahashi J, Misawa M, Murakami M, Mori T, Nomura K, Iwahashi H.  5-Aminolevulinic acid enhances cancer radiotherapy in a mouse tumor model  Springerplus  2013  2:602  PubMed ID: 24324921
7721  Watari K, Nakamura M, Fukunaga Y, Furuno A, Shibata T, Kawahara A, Hosoi F, Kuwano T, Kuwano M, Ono M.  The antitumor effect of a novel angiogenesis inhibitor (an octahydronaphthalene derivative) targeting both VEGF receptor and NF-κB pathway.  Int. J. Cancer  2012  131:310-21  PubMed ID: 21826646
15745  Matsui Y, Amano H, Ito Y, Eshima K, Suzuki T, Ogawa F, Iyoda A, Satoh Y, Kato S, Nakamura M, Kitasato H, Narumiya S, Majima M.  Thromboxane A₂ receptor signaling facilitates tumor colonization through P-selectin-mediated interaction of tumor cells with platelets and endothelial cells  Cancer Sci  2012  103(4):700-7  PubMed ID: 22296266
7656  Fukuda H, Mochizuki S, Abe H, Okano HJ, Hara-Miyauchi C, Okano H, Yamaguchi N, Nakayama M, D'Armiento J, Okada Y.  Host-derived MMP-13 exhibits a protective role in lung metastasis of melanoma cells by local endostatin production.  Br. J. Cancer  2011  105:1615-24  PubMed ID: 22015555



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