Cell No. : Cell Name
RCB2634 : MM46-APR-MUC1 cl.1
update : 2024/01/17
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Comment | Mouse cell line derived from mammary carcinoma. Expressing human MUC1 (APR-MUC1) exogenously. TKG0595 (Deposited from Tohoku Univ.). |
Comment from the depositor | |
Terms and conditions | There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC. |
Remarks | |
Order Form |
Order Form(C-0005.pdf)
 
MTA(C-0007.pdf)
 
MTA(C-0007p.pdf)
 
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Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
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Depositor |
Fukuda, Hiroshi
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Year of deposit |
2007
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Original cell |
TKG0595
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Animal |
_mouse
< Mammals
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Tissue |
mammary
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Disease name |
mammary carcinoma
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Classification |
cancer
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Recombinant |
recombinant
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Exogene |
human MUC1 (APR-MUC1) gene
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History |
Cell Resource Center for Biomedical Research, Tohoku University(TKG0595)
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Lifespan |
infinite
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Morphology |
lymphocyte-like
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Cellosaurus(Expasy) |
CVCL_C514
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deposit info |
lot info |
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Medium |
Medium List |
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Culture type |
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Suspension cells
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Culture method |
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浮遊細胞の培養に関する一般的な注意(Japanese)
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Culture medium |
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RPMI1640 + 10% FBS + 200μg/ml G418
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Antibiotics |
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Free
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Passage method |
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dilution
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Culture information
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Passage ratio |
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1 : 10 split
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SC frequency |
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Subculture : 2 times/week
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Temperature |
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37
℃
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CO2 concentration |
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5
%
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Freeze medium |
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Medium + 10% DMSO
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Freezing method |
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Slow freezing
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Mycoplasma/Acholeplasma |
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(-)
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SSLP(mouse) |
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OK
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Isozyme |
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LD, NP
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Images |
deposit info | lot info |
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Reference information |
Reference |
6
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User's Publication |
1
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Reference |
5262
Batra SK, Kern HF, Worlock AJ, Metzgar RS, Hollingsworth MA.
Transfection of the human Muc 1 mucin gene into a poorly differentiated human pancreatic tumor cell line, Panc1: integration, expression and ultrastructural changes.
J Cell Sci
1991
100 ( Pt 4):841-9
PubMed ID: 1814933
DOI: 10.1242/jcs.100.4.841
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5230
Chang S, Nowinski RC, Nishioka K, Irie RF.
Immunological studies on mouse mammary tumors. VI. Further characterization of a mammary tumor antigen and its distribution in lymphatic cells of allogeneic mice.
Int J Cancer
1972
9(2):409-16
PubMed ID: 5041612
DOI: 10.1002/ijc.2910090220
|
5226
Nishioka K, Irie RF, Inoue M, Chang S, Takeuchi S.
Immunological studies on mouse mammary tumors. I. Induction of resistance to tumor isograft in C3H/He mice.
Int J Cancer
1969
4(2):121-9
PubMed ID: 4899787
DOI: 10.1002/ijc.2910040202
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5227
Takeuchi S, Irie RF, Inoue M, Irie K, Izumi R, Nishioka K.
Immunological studies on mouse mammary tumors. II. Characterization of tumor-specific antibodies against mouse mammary tumors.
Int J Cancer
1969
4(2):130-8
PubMed ID: 5347282
DOI: 10.1002/ijc.2910040203
|
5228
Nishioka K, Irie RF, Kawana T, Takeuchi S.
Immunological studies on mouse mammary tumors. 3. Surface antigens reacting with tumor-specific antibodies in immune adherence.
Int J Cancer
1969
4(2):139-49
PubMed ID: 5347283
DOI: 10.1002/ijc.2910040204
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5229
Irie RF, Nishioka K, Tachibana T, Takeuchi S.
Immunological studies on mouse mammary tumors. IV. Extraction and solubilization of transplantation antigen of mouse mammary tumor.
Int J Cancer
1969
4(2):150-8
PubMed ID: 5347284
DOI: 10.1002/ijc.2910040205
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