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Cell No. : Cell Name
RCB2634 : MM46-APR-MUC1 cl.1  update : 2021/05/20
CommentMouse cell line derived from mammary carcinoma. Expressing human MUC1 (APR-MUC1) exogenously. TKG0595 (Deposited from Tohoku Univ.).
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Fukuda, Hiroshi
Year of deposit 2007
Original cell TKG0595
Animal mouse < Mammals
Tissue mammary
Disease name mammary carcinoma
Classification cancer
Recombinant recombinant
Exogene human MUC1 (APR-MUC1) gene
History Cell Resource Center for Biomedical Research, Tohoku University(TKG0595)
Lifespan infinite
Morphology lymphocyte-like
Cellosaurus(Expasy) CVCL_C514
deposit info
lot info
Medium Medium List
Culture type Suspension cells
Medium and additives RPMI1640 + 10% FBS + 200μg/ml G418
Antibiotics Free
Passage method dilution
Culture information Passage ratio 1 : 10 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
SSLP(mouse) OK
Isozyme LD, NP
Images
deposit info
lot info
Reference information Reference 6
User's Publication 1


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Reference
5262  Batra SK, Kern HF, Worlock AJ, Metzgar RS, Hollingsworth MA.  Transfection of the human Muc 1 mucin gene into a poorly differentiated human pancreatic tumor cell line, Panc1: integration, expression and ultrastructural changes.  J Cell Sci  1991  100 ( Pt 4):841-9  PubMed ID: 1814933  
5230  Chang S, Nowinski RC, Nishioka K, Irie RF.  Immunological studies on mouse mammary tumors. VI. Further characterization of a mammary tumor antigen and its distribution in lymphatic cells of allogeneic mice.  Int J Cancer  1972  9(2):409-16  PubMed ID: 5041612   DOI: 10.1002/ijc.2910090220
5226  Nishioka K, Irie RF, Inoue M, Chang S, Takeuchi S.  Immunological studies on mouse mammary tumors. I. Induction of resistance to tumor isograft in C3H/He mice.  Int J Cancer  1969  4(2):121-9  PubMed ID: 4899787   DOI: 10.1002/ijc.2910040202
5227  Takeuchi S, Irie RF, Inoue M, Irie K, Izumi R, Nishioka K.  Immunological studies on mouse mammary tumors. II. Characterization of tumor-specific antibodies against mouse mammary tumors.  Int J Cancer  1969  4(2):130-8  PubMed ID: 5347282   DOI: 10.1002/ijc.2910040203
5228  Nishioka K, Irie RF, Kawana T, Takeuchi S.  Immunological studies on mouse mammary tumors. 3. Surface antigens reacting with tumor-specific antibodies in immune adherence.  Int J Cancer  1969  4(2):139-49  PubMed ID: 5347283   DOI: 10.1002/ijc.2910040204
5229  Irie RF, Nishioka K, Tachibana T, Takeuchi S.  Immunological studies on mouse mammary tumors. IV. Extraction and solubilization of transplantation antigen of mouse mammary tumor.  Int J Cancer  1969  4(2):150-8  PubMed ID: 5347284   DOI: 10.1002/ijc.2910040205

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User's Publication
8207  Kaori Yoshino, Kaoru Saijo, Chikako Noro and Yukio Nakamura  Development of a simple method to determine the mouse strain from which cultured cell lines originated  Interdisciplinary Bio Central   2010  2:0014    DOI: 10.4051/ibc.2010.2.4.0014



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