Back Back
Cell No. : Cell Name
RCB2630 : B16F10  update : 2020/09/24
CommentMouse cell line derived from melanoma. TKG0348 (Deposited from Tohoku Univ.).
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, a prior written permission of the DEPOSITOR or the RIKEN BRC is necessary.
Order Form Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use.Please use an appropriate MTA(to see).In case of Restriction "a" or "f", please contact RIKEN BRC( regarding any kind of for-profit use.
Basic information Depositor Fukuda, Hiroshi
Originator Fidler, I. J.
Year of deposit 2007
Original cell TKG0348
Animal mouse < Mammals
Strain name C57BL/6J
Tissue skin
Case history melanoma
Classification cancer
History Cell Resource Center for Biomedical Research, Tohoku University(TKG0348)
Lifespan infinite
Morphology epithelial-like
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives RPMI1640 + 10% FBS
Antibiotics Free
Passage method 0.25% trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
SSLP(mouse) OK
Isozyme LD, NP
deposit info
lot info
Reference information Reference 13
User's Publication 20

To topTop
5225  Fan D, Liaw A, Denkins YM, Collins JH, Van Arsdall M, Chang JL, Chakrabarty S, Nguyen D, Kruzel E, Fidler IJ.  Type-1 transforming growth factor-beta differentially modulates tumoricidal activity of murine peritoneal macrophages against metastatic variants of the B16 murine melanoma.  J Exp Ther Oncol  2002  2(5):286-97  PubMed ID: 12416032
5224  Dong Z, Yoneda J, Kumar R, Fidler IJ.  Angiostatin-mediated suppression of cancer metastases by primary neoplasms engineered to produce granulocyte/macrophage colony-stimulating factor.  J Exp Med  1998  188(4):755-63  PubMed ID: 9705957
5223  Esumi N, Fan D, Fidler IJ.  Inhibition of murine melanoma experimental metastasis by recombinant desulfatohirudin, a highly specific thrombin inhibitor.  Cancer Res  1991  51(17):4549-56  PubMed ID: 1873799
5221  Hart IR, Talmadge JE, Fidler IJ.  Comparative studies on the quantitative analysis of experimental metastatic capacity.  Cancer Res  1983  43(1):400-2  PubMed ID: 6291761
5220  Poste G, Doll J, Fidler IJ.  Interactions among clonal subpopulations affect stability of the metastatic phenotype in polyclonal populations of B16 melanoma cells.  Proc Natl Acad Sci U S A  1981  78(10):6226-30  PubMed ID: 6947225
5217  Raz A, Bucana C, McLellan W, Fidler IJ.  Distribution of membrane anionic sites on B16 melanoma variants with differing lung colonising potential.  Nature  1980  284(5754):363-4  PubMed ID: 7360272
5218  Hart IR, Raz A, Fidler IJ.  Effect of cytoskeleton-disrupting agents on the metastatic behavior of melanoma cells.  J Natl Cancer Inst  1980  64(4):891-900  PubMed ID: 6929000
5219  Poste G, Doll J, Hart IR, Fidler IJ.  In vitro selection of murine B16 melanoma variants with enhanced tissue-invasive properties.  Cancer Res  1980  40(5):1636-44  PubMed ID: 7370995
5216  Fidler IJ, Nicolson GL.  Tumor cell and host properties affecting the implantation and survival of blood-borne metastatic variants of B16 melanoma.  Isr J Med Sci  1978  14(1):38-50  PubMed ID: 632082
5215  Fidler IJ, Bucana C.  Mechanism of tumor cell resistance to lysis by syngeneic lymphocytes.  Cancer Res  1977  37(11):3945-56  PubMed ID: 908034
5213  Fidler IJ, Gersten DM, Budmen MB.  Characterization in vivo and in vitro of tumor cells selected for resistance to syngeneic lymphocyte-mediated cytotoxicity.  Cancer Res  1976  36(9 pt.1):3160-5  PubMed ID: 975082
5214  Fidler IJ, Nicolson GL.  Organ selectivity for implantation survival and growth of B16 melanoma variant tumor lines.  J Natl Cancer Inst  1976  57(5):1199-202  PubMed ID: 1003551
5212  Fidler IJ.  Biological behavior of malignant melanoma cells correlated to their survival in vivo.  Cancer Res  1975  35(1):218-24  PubMed ID: 1109790

To topTop
User's Publication
11949  Kozaka S, Tahara Y, Wakabayashi R, Nakata T, Ueda T, Kamiya N, Goto M.  Transcutaneous Cancer Vaccine Using a Reverse Micellar Antigen Carrier.  Mol. Pharm.  2020    PubMed ID: 31833775
12095  Baba K, Nomura M, Ohashi S, Hiratsuka T, Nakai Y, Saito T, Kondo Y, Fukuyama K, Kikuchi O, Yamada A, Matsubara J, Hirohashi K, Mitani Y, Mizumoto A, Muto M.  Experimental model for the irradiation-mediated abscopal effect and factors influencing this effect.  Am J Cancer Res  2020    PubMed ID: 32195019
12122  Makbal R, Villareal MO, Gadhi C, Hafidi A, Isoda H.  Argania Spinosa Fruit Shell Extract-Induced Melanogenesis via cAMP Signaling Pathway Activation.  Int J Mol Sci  2020    PubMed ID: 32268492
12174  Myra O. Villareal, Thanyanan Chaochaiphat, Meriem Bejaoui, Kozo Sato, Hiroko Isoda  Tara Tannin Regulates Pigmentation by Modulating Melanogenesis Enzymes and Melanosome Transport Proteins Expression  Planta Medica  2020   
12201  Irmanida Batubara, Rika Indri Astuti, Muhammad Eka Prastya, Auliya Ilmiawati, Miwa Maeda, Mayu Suzuki, Akie Hamamoto, Hiroshi Takemori  The Antiaging Effect of Active Fractions and Ent-11α-Hydroxy-15-Oxo-Kaur-16-En-19-Oic Acid Isolated from Adenostemma lavenia (L.) O. Kuntze at the Cellular Level  Antioxidants (Basel)  2020  9(8):719  PubMed ID: 32784463
11294  Zhou S, Sakamoto K.  Pyruvic acid/ethyl pyruvate inhibits melanogenesis in B16F10 melanoma cells through PI3K/AKT, GSK3β, and ROS-ERK signaling pathways.  Genes Cells  2019    PubMed ID: 30417494
11549  Chowdhury MR, Moshikur RM, Wakabayashi R, Tahara Y, Kamiya N, Moniruzzaman M, Goto M.  In vivo biocompatibility, pharmacokinetics, antitumor efficacy, and hypersensitivity evaluation of ionic liquid-mediated paclitaxel formulations.  Int J Pharm  2019    PubMed ID: 31077761
11612  Sugiyama Y, Shudo T, Hosokawa S, Watanabe A, Nakano M, Kakizuka A.  Emodin, as a mitochondrial uncoupler, induces strong decreases in adenosine triphosphate (ATP) levels and proliferation of B16F10 cells, owing to their poor glycolytic reserve.  Genes Cells  2019    PubMed ID: 31234244
11645  Yamada H, Hakozaki M, Uemura A, Yamashita T.  Effect of fatty acids on melanogenesis and tumor cell growth in melanoma cells.  J. Lipid Res.  2019    PubMed ID: 31345992
11698  Kazuomi Sato, Yuji Yamaguchi, Setsuko Sakaki, Hiroyuki Takenaka   Pleurochrysis carterae Hot-Water Extract Inhibits Melanogenesis in Murine Melanoma Cells  Cosmetics  2019   
11795  Tan YH, Shudo T, Yoshida T, Sugiyama Y, Si JY, Tsukano C, Takemoto Y, Kakizuka A.  Ellagic acid, extracted from Sanguisorba officinalis, induces G1 arrest by modulating PTEN activity in B16F10 melanoma cells.  Genes Cells  2019    PubMed ID: 31495058
11828  Morikura T, Miyata S.  Effect of Mechanical Compression on Invasion Process of Malignant Melanoma Using In Vitro Three-Dimensional Cell Culture Device.  Micromachines (Basel)  2019    PubMed ID: 31575066
4074    Plasma-Induced Suppression of Recurrent and Reinoculated Melanoma Tumors in Mice  IEEE Transactions on Radiation and Plasma Medical Sciences  2018  2:353 - 359 
4151  Manome Y, Suzuki D, Mochizuki A, Saito E, Sasa K, Yoshimura K, Inoue T, Takami M, Inagaki K, Funatsu T, Kamijo R.  The inhibition of malignant melanoma cell invasion of bone by the TLR7 agonist R848 is dependent upon pro-inflammatory cytokines produced by bone marrow macrophages.  Oncotarget  2018    PubMed ID: 30042824
4158  Shimada K, Ushijima K, Suzuki C, Horiguchi M, Ando H, Akita T, Shimamura M, Fujii J, Yamashita C, Fujimura A.  Pulmonary administration of curcumin inhibits B16F10 melanoma lung metastasis and invasion in mice.  Cancer Chemother. Pharmacol.  2018    PubMed ID: 29869202
4470  Villareal MO, Sato Y, Matsuyama K, Isoda H.  Daphnane diterpenes inhibit the metastatic potential of B16F10 murine melanoma cells in vitro and in vivo.  BMC Cancer  2018  18(1):856  PubMed ID: 30157785
10617  Villareal MO, Kume S, Neffati M, Isoda H.  Upregulation of Mitf by Phenolic Compounds-Rich Cymbopogon schoenanthus Treatment Promotes Melanogenesis in B16 Melanoma Cells and Human Epidermal Melanocytes.  Biomed Res Int  2017  2017:8303671  PubMed ID: 29359158
4527  Yumi Abe, Wakako Takabe, Masayuki Yagi, Akemi Uwaya, Fumiyuki Isami, Yoshikazu Yonei  Inhibition of AGE-induced Melanogenesis in B16 Melanoma Cells by Iridoid-containing Plants  Glycative Stress Research  2017  4 (1): 067-070 
7375  Kim HJ, Kim IS, Dong Y, Lee IS, Kim JS, Kim JS, Woo JT, Cha BY.  Melanogenesis-inducing effect of cirsimaritin through increases in microphthalmia-associated transcription factor and tyrosinase expression.  Int J Mol Sci  2015  16:8772-88  PubMed ID: 25903150
5420  Katoh D, Nishizuka M1, Osada S1, Imagawa M1.  Fad104, a positive regulator of adipocyte differentiation, suppresses invasion and metastasis of melanoma cells by inhibition of STAT3 activity.  PLoS One  2015  10(2):e0117197  PubMed ID: 25671570

Back Back Return Top Page