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Cell No. : Cell Name
RCB2627 : BOY-12E  update : 2022/09/07
CommentHuman cell line derived from urinary bladder carcinoma. TKG0641 (Deposited from Tohoku Univ.).
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor Fukuda, Hiroshi
Originator Kayajima, Tuneyoshi
Year of deposit 2007
Original cell TKG0641
Animal human < Mammals
Genus Homo
Species sapiens
Race Japanese
Gender Male
Age at sampling 66 years
Tissue Urinary bladder
Disease name urinary bladder carcinoma
Classification cancer
History Cell Resource Center for Biomedical Research, Tohoku University(TKG0641)
Lifespan infinite
Morphology epithelial-like
Cellosaurus(Expasy) CVCL_7948
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives MEM + 10% FBS + 0.1mM NEAA
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Isozyme LD, NP
STR(human) OK
deposit info
lot info
Reference information Reference 2
User's Publication 1

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5211  Takemoto M, Shirahama T, Miyauchi T, Matsusako T, Kaneda N, Muramatsu H, Ozawa M, Ohi Y, Muramatsu T.  Metanestin, a glycoprotein with metastasis-associated expression in transitional cell carcinoma of the urinary bladder.  Int J Cancer  1997  74(1):7-14  PubMed ID: 9036862   DOI: 10.1002/(sici)1097-0215(19970220)74:1<7::aid-ijc2>;2-6
5210  T Kayajima, T Shirahama, I Yanase, Y Ohi   Characterization of a new cell line established from a human urinary-bladder cancer, with special reference to metastatic ability to the lung  Jap J Urol Surg  1989  2(6), 577-580   

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User's Publication
14224  Aya Kikuchi, Tomoyuki Suzuki, Taisuke Nakazawa, Masateru Iizuka, Ayako Nakayama, Tohru Ozawa, Minoru Kameda, Nobuaki Shindoh, Tadashi Terasaka, Masaaki Hirano, Sadao Kuromitsu  ASP5878, a selective FGFR inhibitor, to treat FGFR3-dependent urothelial cancer with or without chemoresistance  Cancer Science  2017  108:236-242    DOI: 10.1111/cas.13124

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