Cell No. : Cell Name RCB2627 : BOY-12E  update : 2023/10/16
Comment			Human cell line derived from urinary bladder carcinoma. TKG0641 (Deposited from Tohoku Univ.).
Comment from the depositor
Terms and conditions			There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form			Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)
			Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
	Basic information	Depositor	Fukuda, Hiroshi
		Originator	Kayajima, Tuneyoshi
		Year of deposit	2007
		Original cell	TKG0641
		Animal	_human < Mammals
		Genus	Homo
		Species	sapiens
		Race	Japanese
		Gender	Male
		Age at sampling	66 years
		Tissue	urinary bladder
		Disease name	urinary bladder carcinoma
		Classification	cancer
		History	Cell Resource Center for Biomedical Research, Tohoku University（TKG0641）
		Lifespan	infinite
		Morphology	epithelial-like
		Cellosaurus(Expasy)	CVCL_7948
			deposit info	lot info
		Medium	Medium List
		Culture type		Adherent cells
		Culture medium		MEM + 10% FBS + 0.1mM NEAA
		Antibiotics		Free
		Passage method		0.25% Trypsin
	Culture information	Passage ratio		1 : 8 split
		SC frequency		Subculture : 2 times/week
		Temperature		37 ℃
		CO2 concentration		5 %
		Freeze medium		Medium + 10% DMSO
		Freezing method		Slow freezing
		Mycoplasma		(-)
		Isozyme		LD, NP
		STR(human)		OK
Images			deposit info	lot info
Reference information		Reference	2
		User's Publication	2

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Reference

5211  Takemoto M, Shirahama T, Miyauchi T, Matsusako T, Kaneda N, Muramatsu H, Ozawa M, Ohi Y, Muramatsu T.  Metanestin, a glycoprotein with metastasis-associated expression in transitional cell carcinoma of the urinary bladder.  Int J Cancer  1997  74(1):7-14  PubMed ID: 9036862   DOI: 10.1002/(sici)1097-0215(19970220)74:1<7::aid-ijc2>3.0.co;2-6

5210  T Kayajima, T Shirahama, I Yanase, Y Ohi   Characterization of a new cell line established from a human urinary-bladder cancer, with special reference to metastatic ability to the lung  Jap J Urol Surg  1989  2(6), 577-580

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User's Publication

21489  Myoen S, Mochizuki M, Shibuya-Takahashi R, Fujimori H, Shindo N, Yamaguchi K, Yasuda J, Abe J, Imai T, Sato I, Adachi H, Kawamura S, Ito A, Tamai K.  CD271 promotes proliferation and migration in bladder cancer.  Genes Cells  2023    PubMed ID: 38016691   DOI: 10.1111/gtc.13087

14224  Aya Kikuchi, Tomoyuki Suzuki, Taisuke Nakazawa, Masateru Iizuka, Ayako Nakayama, Tohru Ozawa, Minoru Kameda, Nobuaki Shindoh, Tadashi Terasaka, Masaaki Hirano, Sadao Kuromitsu  ASP5878, a selective FGFR inhibitor, to treat FGFR3-dependent urothelial cancer with or without chemoresistance  Cancer Science  2017  108:236-242  PubMed ID: 27885740   DOI: 10.1111/cas.13124