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Cell No. : Cell Name
RCB2623 : KLN205  update : 2021/02/26
CommentMouse cell line derived from lung squamous cell carcinoma. TKG0214 (Deposited from Tohoku Univ.).
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Fukuda, Hiroshi
Originator Kaneko, T.
Year of deposit 2007
Original cell TKG0214 KLN205(CRL1453)
Animal _mouse < Mammals
Strain name DBA/2
Tissue lung
Disease name squamous cell carcinoma
Classification cancer
History Cell Resource Center for Biomedical Research, Tohoku University(TKG0214)
Lifespan infinite
Morphology epithelial-like
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives MEM + 10% FBS + 0.1mM NEAA
Antibiotics Free
Passage method 0.25% trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
SSLP(mouse) OK
Isozyme LD, NP
Images
deposit info
lot info
Reference information Reference 2
User's Publication 3


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Reference
5197  Kaneko T, LePage GA, Shnitka TK.  KLN205--a murine lung carcinoma cell line.  In Vitro  1980  16(10):884-92  PubMed ID: 7419233
5196  Kaneko T, LePage GA.  Growth characteristics and drug responses of a murine lung carcinoma in vitro and in vivo.  Cancer Res  1978  38(7):2084-90  PubMed ID: 418873

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User's Publication
11603  Aoki N, Matsui Y.  Comprehensive Analysis of Mouse Cancer/Testis Antigen Functions in Cancer Cells and Roles of TEKT5 in Cancer Cells and Testicular Germ Cells.  Mol. Cell. Biol.  2019    PubMed ID: 31208979
10011  Suzawa K, Shien K, Peng H, Sakaguchi M, Watanabe M, Hashida S, Maki Y, Yamamoto H, Tomida S, Soh J, Asano H, Tsukuda K, Nasu Y, Kumon H, Miyoshi S, Toyooka S.  Distant Bystander Effect of REIC/DKK3 Gene Therapy Through Immune System Stimulation in Thoracic Malignancies.  Anticancer Res.  2017  37:301-307  PubMed ID: 28011506
12222  Tomohiro Osaki, Yuki Kawase, Hiroshi Iseki, Shinji Kishimoto, Soko Ikuta, Yoshihiro Muragaki, Masamichi Yamashita, Kazuo Azuma, Yusuke Murahata, Takeshi Tsuka, Norihiko Itoh, Tomohiro Imagawa, Yoshiharu Okamoto  Effects of photodynamic therapy with talaporfin sodium on squamous cell carcinoma and sarcoma cells  Photodiagnosis Photodyn Ther  2017  18:213-220  PubMed ID: 28232036



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