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Cell No. : Cell Name
RCB2621 : M1(D+)  update : 2024/01/17
CommentMouse cell line derived from meyloid leukemia. TKG0319 (Deposited from Tohoku Univ.).
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Fukuda, Hiroshi
Originator Ichikawa, Y.
Year of deposit 2007
Original cell TKG0319
Animal _mouse < Mammals
Strain name SJ
Tissue myeloid leukemia
Classification cancer
History Cell Resource Center for Biomedical Research, Tohoku University(TKG0319)
Lifespan infinite
Morphology lymphocyte-like
Cellosaurus(Expasy) CVCL_C021
deposit info
lot info
Medium Medium List
Culture type Suspension cells
Culture medium RPMI1640 + 10% FBS
Culture method
Antibiotics Free
Passage method dilution
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
SSLP(mouse) OK
Isozyme LD, NP
Reference information Reference 3
User's Publication 0


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Reference
5209  Maeda M, Ichikawa Y, Azuma I.  Differentiation and production of colony-stimulating factor induced by immunostimulants in a leukemia cell line.  J Cell Physiol  1980  105(1):33-8  PubMed ID: 6776132   DOI: 10.1002/jcp.1041050106
5208  Maeda M, Horiuchi M, Numa S, Ichikawa Y.  Characterization of a differentiation-stimulating factor for mouse myeloid leukemia cells.  Gan  1977  68(4):435-47  PubMed ID: 308473  
5207  Ichikawa Y, Maeda M, Horiuchi M.  Induction of differentiated functions which are reversibly suppressed by cytochalasin B.  Exp Cell Res  1975  90(1):20-30  PubMed ID: 123508   DOI: 10.1016/0014-4827(75)90352-3

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