Cell No. : Cell Name
							 RCB2620 : C-1300N18
						
						 update : 2024/08/26
						
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								| Comment | Mouse cell line derived from neuroblastoma.  TKG0307   (Deposited from Tohoku Univ.).  Application consideration | 
							
							
								| Comment from the depositor |  | 
							
							
								| Terms and conditions | There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC. | 
							
							
								| Remarks |  | 
							
							
							
								| Order Form | 
								
									
										
											
										
										Order Form(C-0005.pdf)
										
											
										
										 
									
										
											
										
										MTA(C-0007.pdf)
										
											
										
										 
									
										
											
										
										MTA(C-0007p.pdf)
										
											
										
										 
									
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								| 
									Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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								Basic information
							
							
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                                        Depositor | 
                                
                                
				
				
				
					
						
						
						
							
						
						
						
							
							
							
								Fukuda, Hiroshi
							
							
						
						
							
						
					 | 
				
				
					
					
						
					
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
							
								
									
								
									
										
										
										
										
				
                                
                                
                                
                                        | Year of deposit | 
                                
                                
				
				
				
					
						
						
						
							
						
						
						
							
							
							
								2007
							
							
						
						
							
						
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                                        | Original cell | 
                                
                                
				
				
				
					
						
						
						
							
						
						
						
							
							
							
								TKG0307
							
							
						
						
							
						
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                                        | Animal | 
                                
                                
				
				
				
					
						
						
							
								 
								
								 	_mouse
								
								
								
							
								 
								
								
								 	
								 		< Mammals
								 	
								
								
							
						
						
						
						
						
							
						
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                                        | Strain name | 
                                
                                
				
				
				
					
						
						
						
							
						
						
						
							
							
							
								A/J
							
							
						
						
							
						
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                                        | Tissue | 
                                
                                
				
				
				
					
						
						
						
							
						
						
						
							
							
							
								neuroblastoma
							
							
						
						
							
						
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                                        | Classification | 
                                
                                
				
				
				
					
						
						
						
							cancer
						
						
						
						
							
						
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                                        | History | 
                                
                                
				
				
				
					
						
						
						
							
						
						
						
							
							
							
								Cell Resource Center for Biomedical Research, Tohoku University(TKG0307)
							
							
						
						
							
						
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                                        Lifespan | 
                                
                                
				
				
				
					
						
						
						
							infinite
						
						
						
						
							
						
					 | 
				
				
					
					
						
					
									
								
									
								
									
								
							
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
										
										
										
										
				
                                
                                
                                
                                        | Morphology | 
                                
                                
				
				
				
					
						
						
						
							other
						
						
						
						
							
						
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                                        | Cellosaurus(Expasy) | 
                                
                                
				
				
				
					
						
						
						
							
						
						
						
							
							
							
								CVCL_4724
							
							
						
						
							
						
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											deposit info | 
											lot info | 
											
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												Medium | 
												Medium List | 
											
										
									
								
						
						
						
						
						
					
						
						
						
						
							
							
							
							
								
							
							
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					Culture type | 
				
				
				
				
				
				
					
					
						
						
						
							
						
						
						
						
					
					 | 
					
					
						
						
						
							Adherent/Suspension cells
						
						
						
						
							
						
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					| Culture medium | 
				
				
				
				
				
				
					
					
						
						
						
							
						
						
						
						
					
					 | 
					
					
						
						
						
							
						
						
						
							
							
							
								DMEM (low glucose) + 10% FBS
							
							
						
						
							
						
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					| Antibiotics | 
				
				
				
				
				
				
					
					
						
						
						
							
						
						
						
						
					
					 | 
					
					
						
						
						
							
						
						
						
							
							
							
								Free
							
							
						
						
							
						
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					Passage method | 
				
				
				
				
				
				
					
					
						
						
						
							
						
						
						
						
					
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								pipetting
							
							
						
						
							
						
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								Culture information
							
							
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					Passage ratio | 
				
				
				
				
				
				
					
					
						
						
						
							
						
						
						
						
					
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								1 : 6 split
							
							
						
						
							
						
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					| SC frequency | 
				
				
				
				
				
				
					
					
						
						
						
							
						
						
						
						
					
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								Subculture : 2 times/week
							
							
						
						
							
						
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					| Temperature | 
				
				
				
				
				
				
					
					
						
						
						
							
						
						
						
						
					
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								37
							
							
						
						
							℃
						
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					| CO2 concentration | 
				
				
				
				
				
				
					
					
						
						
						
							
						
						
						
						
					
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								5
							
							
						
						
							%
						
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					| Freeze medium | 
				
				
				
				
				
				
					
					
						
						
						
							
						
						
						
						
					
					 | 
					
					
						
						
						
							
						
						
						
							
							
							
								Medium + 10% DMSO
							
							
						
						
							
						
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					| Freezing method | 
				
				
				
				
				
				
					
					
						
						
						
							
						
						
						
						
					
					 | 
					
					
						
						
						
							Slow freezing
						
						
						
						
							
						
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					| Mycoplasma/Acholeplasma | 
				
				
				
				
				
				
					
					
						
						
						
							
						
						
						
						
					
					 | 
					
					
						
						
						
							(-)
						
						
						
						
							
						
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					| Animal PCR | 
				
				
				
				
				
				
					
					
						
						
						
							
						
						
						
						
					
					 | 
					
					
						
						
						
							
						
						
						
							
							
							
								OK
							
							
						
						
							
						
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					| SSLP(mouse) | 
				
				
				
				
				
				
					
					
						
						
						
							
						
						
						
						
					
					 | 
					
					
						
						
						
							
						
						
						
							
							
							
								OK
							
							
						
						
							
						
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					| Isozyme | 
				
				
				
				
				
				
					
					
						
						
						
							
						
						
						
						
					
					 | 
					
					
						
						
						
							
						
						
						
							
							
							
								LD, NP
							
							
						
						
							
						
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								| Images | 
								deposit info | lot info | 
							
							
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								| Reference information | 
								Reference | 
									
									
									
										6
									
									
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								| User's Publication | 
									
									
									
										1
									
									
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						| Reference | 
						
							
							| 
								
								
								
								1659 
								Etoh T, Ohnuma N, Takahashi H, Taniguchi M 
								[Analysis of cellular immunity of mouse neuroblastoma (C-1300) and human neuroblastoma] 
								Nippon Gan Chiryo Gakkai Shi 
								1982 
								17:1247-54 
								
									
										PubMed ID: 7153616
									
								 
								
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								4688 
								Matsuzawa H, Nirenberg M. 
								Receptor-mediated shifts in cGMP and cAMP levels in neuroblastoma cells. 
								Proc Natl Acad Sci U S A 
								1975 
								72(9):3472-6 
								
									
										PubMed ID: 171662
									
								 
								
									
										DOI: 10.1073/pnas.72.9.3472
									
								
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								4687 
								Amano T, Richelson E, Nirenberg M. 
								Neurotransmitter synthesis by neuroblastoma clones (neuroblast differentiation-cell culture-choline acetyltransferase-acetylcholinesterase-tyrosine hydroxylase-axons-dendrites). 
								Proc Natl Acad Sci U S A 
								1972 
								69(1):258-63 
								
									
										PubMed ID: 4400294
									
								 
								
									
										DOI: 10.1073/pnas.69.1.258
									
								
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								2238 
								Rosenberg RN, Vandeventer L, De Francesco L, Friedkin ME. 
								Regulation of the synthesis of choline-O-acetyltransferase and thymidylate synthetase in mouse neuroblastoma in cell culture 
								Proc Natl Acad Sci U S A 
								1971 
								68(7):1436-40 
								
									
										PubMed ID: 5283932
									
								 
								
									
										DOI: 10.1073/pnas.68.7.1436
									
								
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								4685 
								Blume A, Gilbert F, Wilson S, Farber J, Rosenberg R, Nirenberg M. 
								Regulation of acetylcholinesterase in neuroblastoma cells. 
								Proc Natl Acad Sci U S A 
								1970 
								67(2):786-92 
								
									
										PubMed ID: 5289021
									
								 
								
									
										DOI: 10.1073/pnas.67.2.786
									
								
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								4686 
								Augusti-Tocco G, Sato G. 
								Establishment of functional clonal lines of neurons from mouse neuroblastoma. 
								Proc Natl Acad Sci U S A. 
								1969 
								64(1):311-5 
								
									
										PubMed ID: 5263016
									
								 
								
									
										DOI: 10.1073/pnas.64.1.311
									
								
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