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Cell No. : Cell Name
RCB2620 : C-1300N18  update : 2023/10/16
CommentMouse cell line derived from neuroblastoma. TKG0307 (Deposited from Tohoku Univ.). Application consideration
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor Fukuda, Hiroshi
Year of deposit 2007
Original cell TKG0307
Animal _mouse < Mammals
Strain name A/J
Tissue neuroblastoma
Classification cancer
History Cell Resource Center for Biomedical Research, Tohoku University(TKG0307)
Lifespan infinite
Morphology other
Cellosaurus(Expasy) CVCL_4724
deposit info
lot info
Medium Medium List
Culture type Adherent/Suspension cells
Culture medium DMEM (low glucose) + 10% FBS
Antibiotics Free
Passage method pipetting
Culture information Passage ratio 1 : 6 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Animal PCR OK
SSLP(mouse) OK
Isozyme LD, NP
deposit info
lot info
Reference information Reference 5
User's Publication 1

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1659  Etoh T, Ohnuma N, Takahashi H, Taniguchi M  [Analysis of cellular immunity of mouse neuroblastoma (C-1300) and human neuroblastoma]  Nippon Gan Chiryo Gakkai Shi  1982  17:1247-54  PubMed ID: 7153616  
4688  Matsuzawa H, Nirenberg M.  Receptor-mediated shifts in cGMP and cAMP levels in neuroblastoma cells.  Proc Natl Acad Sci U S A  1975  72(9):3472-6  PubMed ID: 171662   DOI: 10.1073/pnas.72.9.3472
4687  Amano T, Richelson E, Nirenberg M.  Neurotransmitter synthesis by neuroblastoma clones (neuroblast differentiation-cell culture-choline acetyltransferase-acetylcholinesterase-tyrosine hydroxylase-axons-dendrites).  Proc Natl Acad Sci U S A  1972  69(1):258-63  PubMed ID: 4400294   DOI: 10.1073/pnas.69.1.258
4685  Blume A, Gilbert F, Wilson S, Farber J, Rosenberg R, Nirenberg M.  Regulation of acetylcholinesterase in neuroblastoma cells.  Proc Natl Acad Sci U S A  1970  67(2):786-92  PubMed ID: 5289021   DOI: 10.1073/pnas.67.2.786
4686  Augusti-Tocco G, Sato G.  Establishment of functional clonal lines of neurons from mouse neuroblastoma.  Proc Natl Acad Sci U S A.  1969  64(1):311-5  PubMed ID: 5263016   DOI: 10.1073/pnas.64.1.311

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User's Publication
18598  Ikeda E, Matsunaga N, Kakimoto K, Hamamura K, Hayashi A, Koyanagi S, Ohdo S.  Molecular mechanism regulating 24-hour rhythm of dopamine D3 receptor expression in mouse ventral striatum  Mol Pharmacol  2013  83(5):959-67  PubMed ID: 23429911   DOI: 10.1124/mol.112.083535

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