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Cell No. : Cell Name
RCB2619 : L929  update : 2023/12/12
CommentMouse fibroblast-like cell line. TKG0217 (Deposited from Tohoku Univ.).
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor Fukuda, Hiroshi
Originator Earle, W. R.
Year of deposit 2007
Original cell TKG0217
Animal _mouse < Mammals
Genus Mus
Species musculus
Tissue connective tissue
Classification other
History Earle, W. R. -Katsuta, H.- Cell Resource Center for Biomedical Research, Tohoku University(TKG0217)
Lifespan infinite
Morphology fibroblast-like
Cellosaurus(Expasy) CVCL_0462
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Culture medium RPMI1640 + 10% FBS
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
SSLP(mouse) OK
Isozyme LD, NP
deposit info
lot info
Reference information Reference 8
User's Publication 19

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537  Stanley ER, Heard PM.  Factors regulating macrophage production and growth. Purification and some properties of the colony stimulating factor from medium conditioned by mouse L cells.  J Biol Chem  1977  252(12):4305-12  PubMed ID: 301140  
4661  WAYMOUTH C.  Rapid proliferation of sublines of NCTC clone 929 (strain L) mouse cells in a simple chemically defined medium (MB 752/1).  J Natl Cancer Inst  1959  22(5):1003-17  PubMed ID: 13655076   DOI: 10.1093/jnci/22.5.1003
1817  SANFORD KK, HOBBS GL, EARLE WR.  The tumor-producing capacity of strain L mouse cells after 10 years in vitro.  Cancer Res  1956  16(2):162-6  PubMed ID: 13293658  
4660  EAGLE H.  The minimum vitamin requirements of the L and HeLa cells in tissue culture, the production of specific vitamin deficiencies, and their cure.  J Exp Med  1955  102(5):595-600  PubMed ID: 13271674   DOI: 10.1084/jem.102.5.595
1818  EARLE WR, SCHILLING EL, BRYANT JC, EVANS VJ.  The growth of pure strain L cells in fluid-suspension cultures.  J Natl Cancer Inst.  1954  14(5):1159-71  PubMed ID: 13233852  
4659  EVANS VJ, SHANNON JE Jr, BRYANT JC, WALTZ HK, EARLE WR, SANFORD KK.  A quantitative study of the effect of certain chemically defined media on the proliferation in vitro of strain L cells from the mouse.  J Natl Cancer Inst  1953  13(4):773-84  PubMed ID: 13035407  
4658  SANFORD KK, EARLE WR, LIKELY GD.  The growth in vitro of single isolated tissue cells.  J Natl Cancer Inst  1948  9(3):229-46  PubMed ID: 18105872  
4657  Earle, W.R  Production of malignancy in vitro.Ⅳ.The mouse fibroblast cultures and changes seen in the living cells.  J. Nat. Cancer Inst.  1943  4:165-212   

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User's Publication
17815  D. Budrevičius, N. Kajimoto, A. Pakalniškis, K. Tsuru, A. Kareiva, R. Skaudžius  Improvement of luminescent properties of GdPO4 doped with optimal europium concentration by Co-doping with lanthanum  Ceramics International  2023  49:2373-2379    DOI: 10.1016/j.ceramint.2022.09.209
19996  Nishiguchi A, Ito S, Nagasaka K, Taguchi T.  Tissue-Adhesive Decellularized Extracellular Matrix Patches Reinforced by a Supramolecular Gelator to Repair Abdominal Wall Defects  Biomacromolecules  2023  24(4):1545-1554  PubMed ID: 36880637   DOI: 10.1021/acs.biomac.2c01210
17661  Naoki Nishimoto, Junko Fujihara  Improvement of the structural properties and environmental stability of flexible InSb thin films by dopant-assisted crystallization  Applied Physics A  2022  128    DOI: 10.1007/s00339-022-05694-8
16785  Nishiguchi A, Ichimaru H, Ito S, Nagasaka K, Taguchi T.  Hotmelt tissue adhesive with supramolecularly-controlled sol-gel transition for preventing postoperative abdominal adhesion  Acta Biomater  2022  146:80-93  PubMed ID: 35500814   DOI: 10.1016/j.actbio.2022.04.037
19995  Nishiguchi A, Taguchi T.  A pH-driven genipin gelator to engineer decellularized extracellular matrix-based tissue adhesives  Acta Biomater  2021  131:211-221  PubMed ID: 34198010   DOI: 10.1016/j.actbio.2021.06.033
20302  Ebihara T, Xu J, Tonooka Y, Nagasato T, Kakino K, Masuda A, Minamihata K, Kamiya N, Nakatake H, Chieda Y, Mon H, Fujii T, Kusakabe T, Lee JM.  Active Human and Murine Tumor Necrosis Factor α Cytokines Produced from Silkworm Baculovirus Expression System.  Insects  2021  12(6)  PubMed ID: 34199525   DOI: 10.3390/insects12060517
16771  Junko Fujihara, Naoki Nishimoto  Cytotoxicity and pro-inflammatory effect of GaSb thin films in L929 cells  Junko Fujihara, Naoki Nishimoto  2021  35    DOI: 10.1142/s0217979221502970
17101  Nishiguchi A, Taguchi T.  A Thixotropic, Cell-Infiltrative Nanocellulose Hydrogel That Promotes in Vivo Tissue Remodeling  ACS Biomater Sci Eng  2020  6(2):946-958  PubMed ID: 33464861   DOI: 10.1021/acsbiomaterials.9b01549
11065  Naoki Nishimoto Junko Fujihara  Characterization of a Flexible InGaSb/PI Thin Film Grown by RF Magnetron Sputtering and Aqueous Stability Improvement via Surface Coating  physica status solidi   2019  216    DOI: 10.1002/pssa.201800860
4407  Shinichiro Shimomura, Hisao Matsuno, Yohei Kinoshita, Satoshi Fujimura & Keiji Tanaka  Cellular behaviors on polymeric scaffolds with 2D-patterned mechanical properties  Polymer Journalvolume  2018  50, 737–743    DOI: 10.1038/s41428-018-0043-9
4519  Kentaro Yamamoto, Tomoyasu Hirai, Yukari Oda, Daisuke Kawaguchi, Hisao Matsuno, Keiji Tanaka  A Polymer Interfacial Modifier Synthesized by Living Anionic Polymerization: Incorporation of Inorganic Blocks to Chain Ends  Macromol. Chem. Phys.   2017  218:1600473    DOI: 10.1002/macp.201600473
4532  Naoki Nishimotoa, Junko Fujihara, Katsumi Yoshino  Biocompatibility of GaSb thin films grown by RF magnetron sputtering  Applied Surface Science   2017  409, 375-380    DOI: 10.1016/j.apsusc.2017.03.099
14085  Shimomura S, Matsuno H, Sanada K, Tanaka K.  Cell adhesion on glassy scaffolds with a different mechanical response  J Mater Chem B  2017  5(4):714-719  PubMed ID: 32263839   DOI: 10.1039/c6tb02617f
6424  Ando Y, Oku T, Tsuji T.  Platelet Supernatant Suppresses LPS-Induced Nitric Oxide Production from Macrophages Accompanied by Inhibition of NF-κB Signaling and Increased Arginase-1 Expression.  PLoS One  2016  11(9):e0162208  PubMed ID: 27588757   DOI: 10.1371/journal.pone.0162208
6470  Ando Y, Oku T1, Tsuji T1.  Platelets attenuate production of cytokines and nitric oxide by macrophages in response to bacterial endotoxin.  Platelets  2016  27(4):344-50  PubMed ID: 26588084   DOI: 10.3109/09537104.2015.1103369
14087  Shinichiro Shimomura, Hisao Matsuno, Toshiyuki Ohta, Seiichi Kawahara, Keiji Tanaka  Initial Adhesion of Fibroblasts on Thin Rubber Scaffolds  Chemistry Letters  2016  45:475-477    DOI: 10.1246/cl.160061
12251  Keiko Yoshizawa, Ryo Mizuta, Tetsushi Taguchi  Enhanced angiogenesis of growth factor-free porous biodegradable adhesive made with hexanoyl group-modified gelatin  Biomaterials  2015  63:14-23  PubMed ID: 26069904   DOI: 10.1016/j.biomaterials.2015.06.003
15115  Hisao Matsuno, Ruriko Matsuyama, Arisa Yamamoto, Keiji Tanaka  Enhanced cellular affinity for poly (lactic acid) surfaces modified with titanium oxid  Polymer Journal  2015  47:505-512    DOI: 10.1038/pj.2015.30

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