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Cell No. : Cell Name
RCB2618 : BCL1-B20  update : 2023/10/16
CommentMouse cell line derived from B cell leukemia. TKG0435 (Deposited from Tohoku Univ.).
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor Fukuda, Hiroshi
Year of deposit 2007
Original cell TKG0435
Animal _mouse < Mammals
Strain name BALB/c
Tissue B cell leukemia
Disease name spontaneous B cell leukemia
Classification cancer
History Cell Resource Center for Biomedical Research, Tohoku University(TKG0435)
Lifespan infinite
Morphology other
Cellosaurus(Expasy) CVCL_B994
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Culture medium RPMI1640 + 10% FBS
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 1-2 times/week, Medium Renewal : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
SSLP(mouse) OK
Isozyme LD, NP
deposit info
lot info
Reference information Reference 8
User's Publication 3

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5198  Tominaga A, Takahashi T, Kikuchi Y, Mita S, Naomi S, Harada N, Yamaguchi N, Takatsu K.  Role of carbohydrate moiety of IL-5. Effect of tunicamycin on the glycosylation of IL-5 and the biologic activity of deglycosylated IL-5.  J Immunol  1990  144(4):1345-52  PubMed ID: 2303708  
5200  Hitoshi Y, Yamaguchi N, Mita S, Sonoda E, Takaki S, Tominaga A, Takatsu K.  Distribution of IL-5 receptor-positive B cells. Expression of IL-5 receptor on Ly-1(CD5)+ B cells.  J Immunol  1990  144(11):4218-25  PubMed ID: 1692859  
5201  Murata Y1, Yamaguchi N, Hitoshi Y, Tominaga A, Takatsu K.  Interleukin 5 and interleukin 3 induce serine and tyrosine phosphorylations of several cellular proteins in an interleukin 5-dependent cell line.  Biochem Biophys Res Commun  1990  173(3):1102-8  PubMed ID: 2125214   DOI: 10.1016/s0006-291x(05)80899-x
5202  Yamaguchi N, Hitoshi Y, Mita S, Hosoya Y, Murata Y, Kikuchi Y, Tominaga A, Takatsu K.  Characterization of the murine interleukin 5 receptor by using a monoclonal antibody.  Int Immunol  1990  2(2):181-7  PubMed ID: 2088484   DOI: 10.1093/intimm/2.2.181
5206  Migita M, Yamaguchi N, Katoh S, Mita S, Matsumoto R, Sonoda E, Tsuchiya H, Matsuda I, Tominaga A, Takatsu K.  Elevated expression of proto-oncogenes during interleukin-5-induced growth and differentiation of murine B lineage cells.  Microbiol Immunol  1990  34(11):937-52  PubMed ID: 2090920   DOI: 10.1111/j.1348-0421.1990.tb01072.x
1985  Mita S, Tominaga A, Hitoshi Y, Sakamoto K, Honjo T, Akagi M, Kikuchi Y, Yamaguchi N, Takatsu K  Characterization of high-affinity receptors for interleukin 5 on interleukin 5-dependent cell lines.  Proc Natl Acad Sci U S A  1989  86:2311-5  PubMed ID: 2784567   DOI: 10.1073/pnas.86.7.2311
4819  Mita S, Harada N, Naomi S, Hitoshi Y, Sakamoto K, Akagi M, Tominaga A, Takatsu K.  Receptors for T cell-replacing factor/interleukin 5. Specificity, quantitation, and its implication.  J Exp Med  1988  168(3):863-78  PubMed ID: 3262707   DOI: 10.1084/jem.168.3.863
5199  Strober S, Gronowicz ES, Knapp MR, Slavin S, Vitetta ES, Warnke RA, Kotzin B, Schröder J.  Immunobiology of a spontaneous murine B cell leukemia (BCL1).  Immunol Rev  1979  48:169-95  PubMed ID: 121100   DOI: 10.1111/j.1600-065x.1979.tb00303.x

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User's Publication
7581  Hiroi M, Sakaeda Y, Yamaguchi H, Ohmori Y.  Anti-inflammatory cytokine interleukin-4 inhibits inducible nitric oxide synthase gene expression in the mouse macrophage cell line RAW264.7 through the repression of octamer-dependent transcription.  Mediators Inflamm.  2013  2013:369693  PubMed ID: 24459328   DOI: 10.1155/2013/369693
7699  Yokoi H, Kasahara M, Mori K, Ogawa Y, Kuwabara T, Imamaki H, Kawanishi T, Koga K, Ishii A, Kato Y, Mori KP, Toda N, Ohno S, Muramatsu H, Muramatsu T, Sugawara A, Mukoyama M, Nakao K.  Pleiotrophin triggers inflammation and increased peritoneal permeability leading to peritoneal fibrosis.  Kidney Int.  2012  81:160-9  PubMed ID: 21881556   DOI: 10.1038/ki.2011.305
4847  Cho Y, Honda S, Yoshizawa Y, Takagaki K, Usui K, Shibuya A.  Requirement of the cytoplasmic portion for dimer formation of Fcalpha/micro receptor expressed on cell surface.  Mol Immunol  2010  47(4):878-82  PubMed ID: 19945166   DOI: 10.1016/j.molimm.2009.10.016

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